Comparison of qPCR protocols for quantification of “Candidatus Saccharibacteria”, belonging to the Candidate Phyla Radiation, suggests that 23S rRNA is a better target than 16S rRNA

• Published in: PloS one Vol. 19; no. 12; p. e0310675
• Main Authors: Papaleo, Stella, Nodari, Riccardo, Sterzi, Lodovico, D’Auria, Enza, Cattaneo, Camilla, Bettoni, Giorgia, Bonaiti, Clara, Pagliarini, Ella, Zuccotti, Gianvincenzo, Panelli, Simona, Comandatore, Francesco
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.12.2024; Public Library of Science (PLoS)
• Subjects: Bacteria; Biology and life sciences; Comparative analysis; Computer and Information Sciences; Datasets; Deoxyribonucleic acid; DNA; DNA sequencing; DNA, Bacterial - genetics; Experiments; Gene sequencing; Genes; Genetic analysis; Genetic testing; Genetic variability; Genomes; Humans; Identification and classification; Immunomodulation; Medicine and Health Sciences; Microbiota; Microbiota - genetics; Microorganisms; Phylogenetics; Phylogeny; Polymerase chain reaction; Primers; Radiation; Real-Time Polymerase Chain Reaction - methods; Research and Analysis Methods; Ribosomal RNA; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 23S - genetics; rRNA 16S; rRNA 23S; Saccharibacteria; Saliva; Saliva - microbiology; Taxonomy; Tumor necrosis factor-TNF; Varieties; Italy

Candidate Phyla Radiation (CPR) is a large monophyletic group encompassing about 25% of bacterial diversity. Among CPR, "Candidatus Saccharibacteria" is one of the most clinically relevant phyla. Indeed, it is enriched in the oral microbiota of subjects suffering from immune-mediated disor...