Comparison of qPCR protocols for quantification of “Candidatus Saccharibacteria”, belonging to the Candidate Phyla Radiation, suggests that 23S rRNA is a better target than 16S rRNA - Papaleo, Stella; Nodari, Riccardo; Sterzi, Lodovico; D’Auria, Enza; Cattaneo, Camilla; Bettoni, Giorgia; Bonaiti, Clara; Pagliarini, Ella; Zuccotti, Gianvincenzo; Panelli, Simona; Comandatore, Francesco (PloS one, 2024)
Keywords: Bacteria; Biology and life sciences; Comparative analysis; Computer and Information Sciences; Datasets; Deoxyribonucleic acid; DNA; DNA sequencing; DNA, Bacterial - genetics; Experiments; Gene sequencing; Genes; Genetic analysis; Genetic testing; Genetic variability; Genomes; Humans; Identification and classification; Immunomodulation; Medicine and Health Sciences; Microbiota; Microbiota - genetics; Microorganisms; Phylogenetics; Phylogeny; Polymerase chain reaction; Primers; Radiation; Real-Time Polymerase Chain Reaction - methods; Research and Analysis Methods; Ribosomal RNA; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 23S - genetics; rRNA 16S; rRNA 23S; Saccharibacteria; Saliva; Saliva - microbiology; Taxonomy; Tumor necrosis factor-TNF; Varieties; Italy