Nonhomologous tails direct heteroduplex rejection and mismatch correction during single-strand annealing in Saccharomyces cerevisiae

• Published in: PLoS genetics Vol. 20; no. 2; p. e1010527
• Main Authors: Sapède, Elena, Sugawara, Neal, Tyers, Randall G, Nakajima, Yuko, Afreen, Mosammat Faria, Romero Escobar, Jesselin, Haber, James E
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05.02.2024; Public Library of Science (PLoS)
• Subjects: Annealing; Biology and life sciences; Brewer's yeast; DNA helicase; DNA repair; DNA sequencing; Double-strand break repair; Genetic aspects; Genetic research; Genomes; MLH1 protein; MSH2 protein; MSH6 protein; Mutation; Nucleotide sequence; Physical Sciences; Physiological aspects; Proteins; Rad1 protein; Rad52 protein; Research and Analysis Methods; Sequence analysis; Standard deviation; Stem cells; Unwinding; Yeast; United States

Single-strand annealing (SSA) is initiated when a double strand break (DSB) occurs between two flanking repeated sequences, resulting in a deletion that leaves a single copy of the repeat. We studied budding yeast strains carrying two 200-bp URA3 sequences separated by 2.6 kb of spacer DNA (phage la...