Mechanism of MiR-145a-3p/Runx2 pathway in dexamethasone impairment of MC3T3-E1 osteogenic capacity in mice

• Published in: PloS one Vol. 19; no. 11; p. e0309951
• Main Authors: Wu, Hang, Liao, Xinghua, Wu, Tingrui, Xie, Bin, Ding, Sicheng, Chen, Yiren, Song, Lijun, Wei, Bo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.11.2024; Public Library of Science (PLoS)
• Subjects: Alizarin; Alkaline phosphatase; Analysis; Animal experimentation; Animals; Apoptosis; Apoptosis - drug effects; Biology and life sciences; Bone diseases; Bone marrow; Cbfa-1 protein; Cell differentiation; Cell Differentiation - drug effects; Cell Line; Cell Survival - drug effects; Cell viability; Chromosome 3; Collagen (type I); Complications and side effects; Core Binding Factor Alpha 1 Subunit - genetics; Core Binding Factor Alpha 1 Subunit - metabolism; Dexamethasone; Dexamethasone - pharmacology; Diagnosis; Differentiation (biology); Evaluation; Flow cytometry; Genes; Genetic aspects; Growth factors; Kinases; Medicine and Health Sciences; Mice; MicroRNA; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Nodules; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteogenesis; Osteogenesis - drug effects; Osteogenesis - genetics; Osteoporosis; Osteoprotegerin; Oxygen; Pathogenesis; Patient outcomes; Physical Sciences; Polymerase chain reaction; Proteins; Reactive oxygen species; Reactive Oxygen Species - metabolism; Regulatory mechanisms (biology); Reporter gene; Roles; Signal Transduction - drug effects; Staining; Stem cells; Target detection; Transcription factors; China

In this experiment, we screened key miRNAs involved in the dexamethasone-induced decrease in osteogenic capacity of mouse precursor osteoblasts MC3T3-E1 over and investigated their specific regulatory mechanisms. In this experiment, cell counting kit assay was utilized to act on MC3T3-E1 cells at 0,...