The EpiTect Methyl qPCR Assay as novel age estimation method in forensic biology

• Published in: Forensic science international Vol. 264; pp. 132 - 138
• Main Authors: Mawlood, Shakhawan K., Dennany, Lynn, Watson, Nigel, Pickard, Benjamin S.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.07.2016; Elsevier Limited
• Subjects: Adolescent; Adult; Age; Age determination; Aged; Aged, 80 and over; Aging; Aging - genetics; blood; C-Reactive Protein - genetics; CpG Island; CpG Islands - genetics; Deoxyribonucleic acid; digestion; DNA; DNA Methylation; Enzymes; Epigenetics; Female; females; Forensic engineering; Forensic sciences; Gene promoter; Genes; Genomes; genomic islands; Humans; Islands; KCNQ1 Potassium Channel - genetics; loci; longevity; Methylation; Methylation-sensitive restriction; Middle Aged; Nerve Tissue Proteins - genetics; Pathology; promoter regions; Promoter Regions, Genetic; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction; Receptors, AMPA - genetics; restriction endonucleases; Studies; Tripartite Motif Proteins - genetics; Ubiquitin-Protein Ligases - genetics; Young Adult; DNA methylation; Epigenetics; Aging; Gene promoter; Methylation-sensitive restriction; CpG Island

•We report the first application of EpiTect Methyl qPCR system for age estimation.•Age estimation is essential in resolving a variety of forensic and non forensic question.•Outline of methods to estimate donor age using differential DNA methylation regions of gene. Human aging is associated with epi...