Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B

• Published in: Cell research Vol. 25; no. 2; pp. 157 - 168
• Main Authors: Yuan, Pengfei, Zhang, Hongmin, Cai, Changzu, Zhu, Shiyou, Zhou, Yuexin, Yang, Xiaozhou, He, Ruina, Li, Chan, Guo, Shengjie, Li, Shan, Huang, Tuxiong, Perez-Cordon, Gregorio, Feng, Hanping, Wei, Wensheng
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.02.2015; Nature Publishing Group
• Subjects: 631/80/313/1461; 631/80/86; 692/699/255/1911; 692/700/565/1436/2185; Animals; Antibiotic resistance; Antigens - chemistry; Antigens - metabolism; Bacillus; Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; Bacterial Toxins - chemistry; Bacterial Toxins - metabolism; Base Sequence; Biomedical and Life Sciences; Cell Biology; Clostridium difficile; Clostridium difficile - metabolism; Clostridium Infections - metabolism; Clostridium Infections - microbiology; Clostridium Infections - pathology; Cytoskeleton - metabolism; Endocytosis; Gene Knockout Techniques; HEK293 Cells; HeLa Cells; HT29 Cells; Humans; Interleukin-8 - blood; Life Sciences; Mice; Mice, Inbred C57BL; Mice, Knockout; Mortality; Original; original-article; Protein Binding; Protein Structure, Tertiary; Proteoglycans - antagonists & inhibitors; Proteoglycans - chemistry; Proteoglycans - metabolism; RNA, Small Interfering - metabolism; Sulfates; Toxins; 抗生素耐药性; 梭状芽孢杆菌; 毒素; 白细胞介素-8; 硫酸软骨素; 细胞受体; 艰难梭菌; 蛋白聚糖; CSPG4; TcdB; receptor; toxin; CROPs

As a gram-positive, spore-forming anaerobic bacillus, Clostridium difficile （C. difficile） is responsible for severe and fatal pseudomembranous colitis, and poses the most urgent antibiotic resistance threat worldwide. Epidemic C. difficile is the leading cause of antibiotic-associated diarrhoea glo...