Negative regulation of autophagy by UBA6-BIRC6–mediated ubiquitination of LC3

• Published in: eLife Vol. 8
• Main Authors: Jia, Rui, Bonifacino, Juan S
• Format: Journal Article
• Language: English
• Published: England eLife Science Publications, Ltd 06.11.2019; eLife Sciences Publications Ltd; eLife Sciences Publications, Ltd
• Subjects: Autophagy; BIRC6; Cell Biology; Cell Line; CRISPR; Enzymes; Gene expression; Gene Expression Regulation, Enzymologic; Gene Knockout Techniques; Genetic Testing; Genomes; Genomics; Hippocampus; Humans; Inhibitor of Apoptosis Proteins - metabolism; Kinases; LC3; Ligases; Microtubule-Associated Proteins - metabolism; Nervous system diseases; Neurodegenerative diseases; Neurons; Novels; Phagocytosis; Phosphorylation; Polymerization; Proteasome Endopeptidase Complex - metabolism; Proteasomes; Protein biosynthesis; Protein Interaction Maps; Protein synthesis; Proteins; Proteolysis; Scientific equipment industry; Sedimentation & deposition; Synuclein; UBA6; Ubiquitin; Ubiquitin-Activating Enzymes - metabolism; Ubiquitin-conjugating enzyme; Ubiquitin-protein ligase; Ubiquitination; Variance analysis; United States; United States > US; autophagy; cell biology; rat; UBA6; ubiquitination; BIRC6; LC3; human

Although the process of autophagy has been extensively studied, the mechanisms that regulate it remain insufficiently understood. To identify novel autophagy regulators, we performed a whole-genome CRISPR/Cas9 knockout screen in H4 human neuroglioma cells expressing endogenous LC3B tagged with a tan...