同位素稀释-超高效液相色谱-串联质谱技术分析人血浆中利奈唑胺的浓度
目的：建立超高效液相色谱-串联质谱法和同位素稀释内标法分析人血浆中利奈唑胺浓度的方法.方法：血浆样本经乙腈稀释沉淀蛋白进行前处理,以同位素利奈唑胺-d3为内标,采用Acquity UPLC（c）BEH-C18色谱柱（2.1 mm×50mm,1.7 μm）分离,流动相包括水（含0.1％甲酸,V/V）和乙腈（含0.1％甲酸,V/V）,梯度洗脱,流速为0.3 ml/min.正离子检测模式下,采用多离子反应监测模式进行分析.结果：利奈唑胺浓度在0.078～10.000 μg/ml范围内线性关系良好,定量限为0.078 μg/ml;日内、日间RSD均＜6％;提取回收率〉90％.结论：本方法操作简便、快速...
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| Published in | 中国医院用药评价与分析 Vol. 17; no. 5; pp. 581 - 583 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
卫生部中日友好医院药学部,北京,100029%延边大学附属医院药学部,吉林 延吉,133000
2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-2124 |
| DOI | 10.14009/j.issn.1672-2124.2017.05.002 |
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| Abstract | 目的：建立超高效液相色谱-串联质谱法和同位素稀释内标法分析人血浆中利奈唑胺浓度的方法.方法：血浆样本经乙腈稀释沉淀蛋白进行前处理,以同位素利奈唑胺-d3为内标,采用Acquity UPLC（c）BEH-C18色谱柱（2.1 mm×50mm,1.7 μm）分离,流动相包括水（含0.1％甲酸,V/V）和乙腈（含0.1％甲酸,V/V）,梯度洗脱,流速为0.3 ml/min.正离子检测模式下,采用多离子反应监测模式进行分析.结果：利奈唑胺浓度在0.078～10.000 μg/ml范围内线性关系良好,定量限为0.078 μg/ml;日内、日间RSD均＜6％;提取回收率〉90％.结论：本方法操作简便、快速、准确、专属性强且稳定性良好,适用于人血浆中利奈唑胺浓度的分析,可用于临床治疗药物监测. |
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| AbstractList | R917; 目的:建立超高效液相色谱-串联质谱法和同位素稀释内标法分析人血浆中利奈唑胺浓度的方法.方法:血浆样本经乙腈稀释沉淀蛋白进行前处理,以同位素利奈唑胺-d3为内标,采用Acquity UPLC(c)BEH-C18色谱柱(2.1 mm×50 mm,1.7 μm)分离,流动相包括水(含0.1％甲酸,V/V)和乙腈(含0.1％甲酸,V/V),梯度洗脱,流速为0.3 ml/min.正离子检测模式下,采用多离子反应监测模式进行分析.结果:利奈唑胺浓度在0.078～10.000 μg/ml范围内线性关系良好,定量限为0.078 μg/ml;日内、日间RSD均<6％;提取回收率>90％.结论:本方法操作简便、快速、准确、专属性强且稳定性良好,适用于人血浆中利奈唑胺浓度的分析,可用于临床治疗药物监测. 目的：建立超高效液相色谱-串联质谱法和同位素稀释内标法分析人血浆中利奈唑胺浓度的方法.方法：血浆样本经乙腈稀释沉淀蛋白进行前处理,以同位素利奈唑胺-d3为内标,采用Acquity UPLC（c）BEH-C18色谱柱（2.1 mm×50mm,1.7 μm）分离,流动相包括水（含0.1％甲酸,V/V）和乙腈（含0.1％甲酸,V/V）,梯度洗脱,流速为0.3 ml/min.正离子检测模式下,采用多离子反应监测模式进行分析.结果：利奈唑胺浓度在0.078～10.000 μg/ml范围内线性关系良好,定量限为0.078 μg/ml;日内、日间RSD均＜6％;提取回收率〉90％.结论：本方法操作简便、快速、准确、专属性强且稳定性良好,适用于人血浆中利奈唑胺浓度的分析,可用于临床治疗药物监测. |
| Abstract_FL | Objective:To establish a method for analysis of content of linezolid in human plasma by ultra performance liquid chromatography-tandem mass spectrometry with isotopes dilution.METHODS: Plasma samples were disposed by acetonitrile dilution protein deposition,isotope rina thiazole amine-d3 was set as internal control,and Acquity UPLC(c)BEH-C18 column(2.1 mm×50 mm,1.7 μm)was adopted,the mobile phase consisted of water(0.1％formic acid,V/V)and acetonitrile(0.1％formic acid,V/V)in gradient elution,at flow rate of 0.3 ml/min.Under positive ion detection mode,multiple ion reaction monitoring mode was adopted for analysis.RESULTS: The content of linezolid showed good linear relationship within 0.078 μg/ml-10.000 μg/ml,the limit of quantitation was 0.078 μg/ml.And RSD of intra-and inter-day precision <6％,the recovery >90％.CONCLUSIONS: This method is simple,rapid and accurate with strong specificity and good stability,which can be used for analysis of content of linezolid in human plasma and clinical therapeutic drug monitoring. |
| Author | 王晓雪 车仙花 崔刚 张相林 |
| AuthorAffiliation | 卫生部中日友好医院药学部,北京100029 延边大学附属医院药学部,吉林延吉133000 |
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| Author_FL | WANG Xiaoxue CUI Gang CHE Xianhua ZHANG Xianglin |
| Author_FL_xml | – sequence: 1 fullname: WANG Xiaoxue – sequence: 2 fullname: CHE Xianhua – sequence: 3 fullname: CUI Gang – sequence: 4 fullname: ZHANG Xianglin |
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| DocumentTitleAlternate | Analysis on Content of Linezolid in Human Plasma by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry with Isotopes Dilution |
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| Keywords | Linezolid Ultra performance liquid chromatography-tandem mass spectrometry Isotopes dilution 超高效液相色谱-串联质谱法 同位素稀释内标法 利奈唑胺 |
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| Notes | Objective：To establish a method for analysis of content of linezolid in human plasma by ultra performance liquid chromatography-tandem mass spectrometry with isotopes dilution.METHODS： Plasma samples were disposed by acetonitrile dilution protein deposition,isotope rina thiazole amine-d3 was set as internal control,and Acquity UPLC（c）BEH-C18 column（2.1 mm×50 mm,1.7 μm）was adopted,the mobile phase consisted of water（0.1%formic acid,V/V）and acetonitrile（0.1%formic acid,V/V）in gradient elution,at flow rate of 0.3 ml/min.Under positive ion detection mode,multiple ion reaction monitoring mode was adopted for analysis.RESULTS： The content of linezolid showed good linear relationship within 0.078 μg/ml-10.000 μg/ml,the limit of quantitation was 0.078 μg/ml.And RSD of intra-and inter-day precision 〈6%,the recovery 〉90%.CONCLUSIONS： This method is simple,rapid and accurate with strong specificity and good stability,which can be used for analysis of content of linezolid in human plasma and clinical therapeutic drug mon |
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| PublicationTitle | 中国医院用药评价与分析 |
| PublicationTitleAlternate | Evaluation and Analysis of Drug-use in Hospitals of China |
| PublicationTitle_FL | Evaluation and Analysis of Drug-Use in Hospitals of China |
| PublicationYear | 2017 |
| Publisher | 卫生部中日友好医院药学部,北京,100029%延边大学附属医院药学部,吉林 延吉,133000 |
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| Snippet | 目的：建立超高效液相色谱-串联质谱法和同位素稀释内标法分析人血浆中利奈唑胺浓度的方法.方法：血浆样本经乙腈稀释沉淀蛋白进行前处理,以同位素利奈唑胺-d3为内标,采用Acquity UPLC（c）BEH-C18色谱柱（2.1 mm×50mm,1.7... R917; 目的:建立超高效液相色谱-串联质谱法和同位素稀释内标法分析人血浆中利奈唑胺浓度的方法.方法:血浆样本经乙腈稀释沉淀蛋白进行前处理,以同位素利奈唑胺-d3为内标,采用Acquity UPLC(c)BEH-C18色谱柱(2.1 mm×50 mm,1.7... |
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| SubjectTerms | 利奈唑胺 同位素稀释内标法 超高效液相色谱-串联质谱法 |
| Title | 同位素稀释-超高效液相色谱-串联质谱技术分析人血浆中利奈唑胺的浓度 |
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