Matching of oligoclonal immunoglobulin transcriptomes and proteomes of cerebrospinal fluid in multiple sclerosis

• Published in: Nature medicine Vol. 14; no. 6; pp. 688 - 693
• Main Authors: Obermeier, Birgit, Mentele, Reinhard, Malotka, Joachim, Kellermann, Josef, Kümpfel, Tania, Wekerle, Hartmut, Lottspeich, Friedrich, Hohlfeld, Reinhard, Dornmair, Klaus
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.06.2008; Nature Publishing Group
• Subjects: Amino Acid Sequence; B-Lymphocytes - immunology; Biomedical and Life Sciences; Biomedicine; Body fluids; Cancer Research; Care and treatment; Cellular biology; Cerebrospinal fluid; Cerebrospinal Fluid Proteins - genetics; Cerebrospinal Fluid Proteins - immunology; Databases, Genetic; Diagnosis; Gels; Health aspects; Humans; Immunoglobulin Heavy Chains - genetics; Immunoglobulin Light Chains - genetics; Immunoglobulin Variable Region - genetics; Immunoglobulins; Infectious Diseases; Isoelectric Focusing; Mass Spectrometry; Medical research; Metabolic Diseases; Molecular Medicine; Molecular Sequence Data; Multiple sclerosis; Multiple Sclerosis - cerebrospinal fluid; Multiple Sclerosis - immunology; Mutation; Neurosciences; Oligoclonal Bands - genetics; Oligoclonal Bands - immunology; Peptides; Proteome - analysis; Proteomics; Research methodology; Risk factors; Sequence Homology, Amino Acid; Spine; technical-report; Transcription, Genetic; Germany

We describe a method for correlating the immunoglobulin (Ig) proteomes with the B cell transcriptomes in human fluid and tissue samples, using multiple sclerosis as a paradigm. Oligoclonal Ig bands and elevated numbers of clonally expanded B cells in the cerebrospinal fluid (CSF) are diagnostic hall...