Detection of Babesia bigemina-infected carriers by polymerase chain reaction amplification

• Published in: Journal of Clinical Microbiology Vol. 30; no. 10; pp. 2576 - 2582
• Main Authors: Figueroa, J.V. (Centro Nacional de Investigacion Disciplinaria-Parasitologia, Morelos, Mexico), Chieves, L.P, Johnson, G.S, Buening, G.M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.10.1992
• Subjects: ADN; Animals; Babesia - genetics; Babesia - isolation & purification; BABESIA BIGEMINA; Babesiosis - diagnosis; Base Sequence; BIOCHIMIE; Biological and medical sciences; BIOQUIMICA; BOVIN; Carrier State - diagnosis; Carrier State - veterinary; Cattle; Cattle Diseases - diagnosis; DIAGNOSTIC; DIAGNOSTICO; DNA, Protozoan - blood; EPIDEMIOLOGIA; EPIDEMIOLOGIE; ERITROCITOS; ERYTHROCYTE; Erythrocytes - parasitology; Fundamental and applied biological sciences. Psychology; GANADO BOVINO; Molecular Sequence Data; Polymerase Chain Reaction - methods; Protozoa; Sensitivity and Specificity; Infection; Polymerase chain reaction; Protozoa; Babesia bigemina; Protozoal disease; Detection; Sporozoa

A SpeI-AvaI fragment (0.3 kbp) from pBbi16 (a pBR322 derivative containing a 6.3-kbp Babesia bigemina DNA insert) was subcloned into the pBluescript phagemid vector and was sequenced by the dideoxy-mediated chain termination method. Two sets of primers were designed for the polymerase chain reaction...