Sequence-Specific Binding of Human Ets-1 to the T Cell Receptor α Gene Enhancer

• Published in: Science (American Association for the Advancement of Science) Vol. 250; no. 4982; pp. 814 - 818
• Main Authors: I-Cheng Ho, Bhat, Narayan K., Gottschalk, Lisa R., Lindsten, Tullia, Thompson, Craig B., Papas, Takis S., Leiden, Jeffrey M.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 09.11.1990; American Association for the Advancement of Science
• Subjects: Antigen receptors, T cell; Base Sequence; Binding sites; Binding, Competitive; Biological and medical sciences; Cloning, Molecular; Complementary DNA; DNA; DNA - genetics; DNA Mutational Analysis; DNA probes; Enhancer Elements, Genetic; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Gene Rearrangement, T-Lymphocyte; Genes; Genes. Genome; Genetic mutation; Humans; Immunoblotting; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Nuclear proteins; Oligonucleotide probes; Protein binding; Proto-Oncogene Protein c-ets-1; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; Proto-Oncogene Proteins c-ets; Proto-Oncogenes; Receptors; Receptors, Antigen, T-Cell - genetics; Receptors, Antigen, T-Cell - metabolism; T cell antigen receptors; T cells; T lymphocytes; Transcription Factors; Transcription, Genetic; Human; Binding; Sequence specificity; Immunogenetics; Enhancer sequence; T cell receptor; Gene; C-Onc gene; DNA binding protein; Molecular interaction; Gel retardation technique; Property structure relationship

Expression of the human T cell receptor (TCR) α gene is regulated by a T cell-specific transcriptional enhancer that is located 4.5 kilobases (kb) 3′ to the Cα gene segment. The core enhancer contains two nuclear protein binding sites, Tα1 and Tα2, which are essential for full enhancer activity. Tα1...