Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea

• Published in: Biotechnology for biofuels Vol. 8; no. 1; p. 94
• Main Authors: Kasai, Yuki, Oshima, Kohei, Ikeda, Fukiko, Abe, Jun, Yoshimitsu, Yuya, Harayama, Shigeaki
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 30.06.2015; BioMed Central
• Subjects: agar; auxotrophs; biodiesel; Biomass energy; breeding; Chlorophyta; Cloning; complementary DNA; fuel production; genes; genetic engineering; genetic transformation; Genetically modified organisms; Laws, regulations and rules; Lipids; microalgae; mutagenesis; plasmids; Ponds; Protozoa; Pyrimidines; quantitative polymerase chain reaction; ribulose-bisphosphate carboxylase; transcription (genetics); tubulin; Tubulins; ultraviolet radiation; uracil; uridine monophosphate; Genetic transformation; Uracil auxotroph; Self-cloning; Pseudochoricystis ellipsoidea; Molecular breeding; Green algae; Microalgae

Microalgae have received considerable interest as a source of biofuel production. The unicellular green alga Pseudochoricystis ellipsoidea (non-validated scientific name) strain Obi appears to be suitable for large-scale cultivation in outdoor open ponds for biodiesel production because it accumulat...