Evaluating sources of technical variability in the mechano-node-pore sensing pipeline and their effect on the reproducibility of single-cell mechanical phenotyping

• Published in: PloS one Vol. 16; no. 10; p. e0258982
• Main Authors: Li, Brian, Cotner, Kristen L., Liu, Nathaniel K., Hinz, Stefan, LaBarge, Mark A., Sohn, Lydia L.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.10.2021; Public Library of Science (PLoS)
• Subjects: Analysis; Bioengineering; Biology and Life Sciences; Biosensors; Cell physiology; Computer and Information Sciences; Data processing; Deformation; Design; Engineering and Technology; Flow Cytometry; Laboratory tests; Mechanical engineering; Mechanical measurement; Mechanical properties; Mechanical tests; Medicine and Health Sciences; Microfluidics; Microfluidics - methods; Nodes; Performance evaluation; Phenotype; Phenotyping; Platforms; Reproducibility; Reproducibility of Results; Research and Analysis Methods; Single-Cell Analysis; Social Sciences; Trends; Variability; Viscoelasticity; United States; United States > US; San Francisco California; California
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0258982

Cellular mechanical properties can reveal physiologically relevant characteristics in many cell types, and several groups have developed microfluidics-based platforms to perform high-throughput single-cell mechanical testing. However, prior work has performed only limited characterization of these p...