BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis

• Published in: Nature (London) Vol. 527; no. 7577; pp. 192 - 197
• Main Authors: Canver, Matthew C., Smith, Elenoe C., Sher, Falak, Pinello, Luca, Sanjana, Neville E., Shalem, Ophir, Chen, Diane D., Schupp, Patrick G., Vinjamur, Divya S., Garcia, Sara P., Luc, Sidinh, Kurita, Ryo, Nakamura, Yukio, Fujiwara, Yuko, Maeda, Takahiro, Yuan, Guo-Cheng, Zhang, Feng, Orkin, Stuart H., Bauer, Daniel E.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.11.2015; Nature Publishing Group
• Subjects: 13/100; 13/109; 13/31; 38/22; 38/70; 38/77; 38/88; 45/23; 49/47; 631/1647/1511; 631/208/200; 631/337/572/2102; 64/60; 692/308/2056; Animals; Base Sequence; Carrier Proteins - genetics; Cells, Cultured; Clustered Regularly Interspaced Short Palindromic Repeats - genetics; CRISPR-Associated Proteins - metabolism; CRISPR-Cas Systems - genetics; DNA methylation; DNA-Binding Proteins; Enhancer Elements, Genetic - genetics; Erythroblasts - metabolism; Fetal Hemoglobin - genetics; Gene expression; Genetic diversity; Genetic Engineering; Genome - genetics; Genomes; Genomics; Humanities and Social Sciences; Humans; Methods; Mice; Molecular Sequence Data; multidisciplinary; Mutagenesis; Mutagenesis - genetics; Nuclear Proteins - genetics; Organ Specificity; Physiological aspects; Repressor Proteins; Reproducibility of Results; RNA, Guide, CRISPR-Cas Systems - genetics; Science; Species Specificity; Studies

Enhancers, critical determinants of cellular identity, are commonly recognized by correlative chromatin marks and gain-of-function potential, although only loss-of-function studies can demonstrate their requirement in the native genomic context. Previously, we identified an erythroid enhancer of hum...