PANDORA-seq expands the repertoire of regulatory small RNAs by overcoming RNA modifications

• Published in: Nature cell biology Vol. 23; no. 4; pp. 424 - 436
• Main Authors: Shi, Junchao, Zhang, Yunfang, Tan, Dongmei, Zhang, Xudong, Yan, Menghong, Zhang, Ying, Franklin, Reuben, Shahbazi, Marta, Mackinlay, Kirsty, Liu, Shichao, Kuhle, Bernhard, James, Emma R., Zhang, Liwen, Qu, Yongcun, Zhai, Qiwei, Zhao, Wenxin, Zhao, Linlin, Zhou, Changcheng, Gu, Weifeng, Murn, Jernej, Guo, Jingtao, Carrell, Douglas T., Wang, Yinsheng, Chen, Xuemei, Cairns, Bradley R., Yang, Xiang-lei, Schimmel, Paul, Zernicka-Goetz, Magdalena, Cheloufi, Sihem, Zhou, Tong, Chen, Qi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.04.2021; Nature Publishing Group
• Subjects: 38/71; 42/109; 45/100; 45/29; 45/90; 45/91; 631/136/2435; 631/1647/514/1949; 631/337/384; 631/337/384/521; 64/60; 82/58; Adapters; Biomedical and Life Sciences; Cancer Research; Cell Biology; Cellular control mechanisms; Combinatorial analysis; Complementary DNA; Deoxyribonucleic acid; Developmental Biology; DNA; DNA sequencing; DNA, Complementary - genetics; Embryo cells; Genetic aspects; HeLa Cells; Humans; Identification and classification; Life Sciences; Methods; MicroRNAs - genetics; miRNA; Non-coding RNA; Physiological aspects; Pluripotency; Resource; Reverse transcription; Ribonucleic acid; RNA; RNA modification; RNA Processing, Post-Transcriptional - genetics; RNA sequencing; RNA, Ribosomal - genetics; RNA, Small Untranslated - genetics; RNA-Seq; rRNA; Spleen; Stem cell transplantation; Stem Cells; Transcriptome - genetics; Transfer RNA; United States

Although high-throughput RNA sequencing (RNA-seq) has greatly advanced small non-coding RNA (sncRNA) discovery, the currently widely used complementary DNA library construction protocol generates biased sequencing results. This is partially due to RNA modifications that interfere with adapter ligati...