Phage combination therapies for bacterial wilt disease in tomato

• Published in: Nature biotechnology Vol. 37; no. 12; pp. 1513 - 1520
• Main Authors: Wang, Xiaofang, Wei, Zhong, Yang, Keming, Wang, Jianing, Jousset, Alexandre, Xu, Yangchun, Shen, Qirong, Friman, Ville-Petri
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.12.2019; Nature Publishing Group
• Subjects: 631/158/1745; 631/158/2456; 631/158/855; 631/326/2565/2134; 631/61/168; Agriculture; Bacteria; Bacterial diseases of plants; Bacteriophages; Bacteriophages - genetics; Bioinformatics; Biological control; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Care and treatment; Diseases and pests; Environmental aspects; Field tests; Incidence; Life Sciences; Lycopersicon esculentum - microbiology; Microbiomes; Microbiota; Pathogens; Pest Control, Biological - methods; Pesticides; Phages; Plant Diseases - microbiology; Ralstonia solanacearum - genetics; Ralstonia solanacearum - pathogenicity; Ralstonia solanacearum - virology; Rhizosphere; Tomatoes; Wilt; China
• ISSN: 1087-0156; 1546-1696; 1546-1696
• DOI: 10.1038/s41587-019-0328-3

Bacteriophages have been proposed as an alternative to pesticides to kill bacterial pathogens of crops. However, the efficacy of phage biocontrol is variable and poorly understood in natural rhizosphere microbiomes. We studied biocontrol efficacy of different phage combinations on Ralstonia solanacearum infection in tomato. Increasing the number of phages in combinations decreased the incidence of disease by up to 80% in greenhouse and field experiments during a single crop season. The decreased incidence of disease was explained by a reduction in pathogen density and the selection for phage-resistant but slow-growing pathogen strains, together with enrichment for bacterial species that were antagonistic toward R. solanacearum . Phage treatment did not affect the existing rhizosphere microbiota. Specific phage combinations have potential as precision tools to control plant pathogenic bacteria. Phage combination therapies reduce bacterial wilt disease incidence in tomato by up to 80%.