Hexokinase II dissociation alone cannot account for changes in heart mitochondrial function, morphology and sensitivity to permeability transition pore opening following ischemia

• Published in: PloS one Vol. 15; no. 6; p. e0234653
• Main Authors: Pereira, Gonçalo C., Lee, Laura, Rawlings, Nadiia, Ouwendijk, Joke, Parker, Joanne E., Andrienko, Tatyana N., Henley, Jeremy M., Halestrap, Andrew P.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 24.06.2020; Public Library of Science (PLoS)
• Subjects: Animals; Biology and Life Sciences; Cell Respiration - drug effects; Development and progression; Dynamins - metabolism; Glucose-6-Phosphate - pharmacology; Health aspects; Hemodynamics - drug effects; Hexokinase; Hexokinase - metabolism; Hydrogen-Ion Concentration; Ischemic Preconditioning; Ligands; Male; Medicine and Health Sciences; Mitochondria; Mitochondria, Heart - drug effects; Mitochondria, Heart - enzymology; Mitochondria, Heart - ultrastructure; Mitochondrial Dynamics - drug effects; Mitochondrial Membrane Transport Proteins - metabolism; Mitochondrial Membranes - drug effects; Mitochondrial Membranes - metabolism; Mitochondrial Permeability Transition Pore; Myocardial ischemia; Myocardial Ischemia - enzymology; Myocardial Ischemia - pathology; Physical Sciences; Protein Binding - drug effects; Rats, Wistar; Research and Analysis Methods; United Kingdom

We previously demonstrated that hexokinase II (HK2) dissociation from mitochondria during cardiac ischemia correlates with cytochrome c (cyt-c) loss, oxidative stress and subsequent reperfusion injury. However, whether HK2 release is the primary signal mediating this ischemia-induced mitochondrial d...