Effects of pH and Polysaccharides on Peptide Binding to Class II Major Histocompatibility Complex Molecules

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 88; no. 7; pp. 2740 - 2744
• Main Authors: Harding, Clifford V., Roof, Richard W., Allen, Paul M., Unanue, Emil R.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.04.1991; National Acad Sciences
• Subjects: 550201 - Biochemistry- Tracer Techniques; ANIMAL CELLS; Animals; Antigen presentation; Antigen-Presenting Cells - immunology; ANTIGENS; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; Biological and medical sciences; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOHYDRATES; CARBOXYLIC ACIDS; Cell Line; Cell Membrane - immunology; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; Dextrans; ELECTRON CAPTURE RADIOISOTOPES; Endosomes; ENZYMES; Fundamental and applied biological sciences. Psychology; Fundamental immunology; GLOBINS; GLYCOSYL HYDROLASES; HEMOGLOBIN; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; Histocompatibility antigens (hla, h-2 and other systems); Histocompatibility Antigens Class II - metabolism; HISTOCOMPATIBILITY COMPLEX; HYDROGEN COMPOUNDS; Hydrogen-Ion Concentration; HYDROLASES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE 125; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; Kinetics; LEUKOCYTES; LYMPHOCYTES; Lysosomes; LYSOZYME; MACROPHAGES; Macrophages - immunology; MATERIALS; Mice; Mice, Inbred CBA; Molecular immunology; Molecules; Muramidase - immunology; Muramidase - metabolism; NUCLEI; O-GLYCOSYL HYDROLASES; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PEPTIDES; PH VALUE; PHAGOCYTES; PIGMENTS; POLYSACCHARIDES; Polysaccharides - pharmacology; PORPHYRINS; Protein Binding; PROTEINS; RADIOISOTOPES; RADIORECEPTOR ASSAY; SACCHARIDES; SOMATIC CELLS; T lymphocytes; TRACER TECHNIQUES; TRITIUM COMPOUNDS; Binding; Molecular processing; Peptides; Major histocompatibility system; Rodentia; Class II histocompatibility antigen; Molecular interaction; Antigen; Vertebrata; Mammalia; Mouse; PH; Polysaccharide

The binding of immunogenic peptides to class II major histocompatibility molecules was examined at various pH values. We studied binding of peptides containing residues 52-61 from hen egg lysozyme (HEL) to I-Akon fixed peritoneal macrophages or to solubilized affinity-purified I-Ak. Optimum binding...