Quantitation of lipid phases in phospholipid vesicles by the generalized polarization of Laurdan fluorescence

• Published in: Biophysical journal Vol. 60; no. 1; pp. 179 - 189
• Main Authors: Parasassi, T., De Stasio, G., Ravagnan, G., Rusch, R.M., Gratton, E.
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.07.1991; Biophysical Society
• Subjects: 2-Naphthylamine - analogs & derivatives; Biological and medical sciences; fluorescence; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; Kinetics; Laurates; lipids; Liposomes; Molecular biophysics; Molecular Conformation; Phospholipids - chemistry; Solvents; Spectrometry, Fluorescence - methods; Thermodynamics; Vesicle; Phospholipid; Emission spectrum; Fluorescence spectrometry; Quantitative analysis

The sensitivity of Laurdan (6-dodecanoyl-2-dimethylaminonaphthalene) excitation and emission spectra to the physical state of the membrane arises from dipolar relaxation processes in the membrane region surrounding the Laurdan molecule. Experiments performed using phospholipid vesicles composed of p...