A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection

• Published in: Nature communications Vol. 12; no. 1; pp. 4635 - 13
• Main Authors: Li, Tingting, Cai, Hongmin, Yao, Hebang, Zhou, Bingjie, Zhang, Ning, van Vlissingen, Martje Fentener, Kuiken, Thijs, Han, Wenyu, GeurtsvanKessel, Corine H., Gong, Yuhuan, Zhao, Yapei, Shen, Quan, Qin, Wenming, Tian, Xiao-Xu, Peng, Chao, Lai, Yanling, Wang, Yanxing, Hutter, Cedric A. J., Kuo, Shu-Ming, Bao, Juan, Liu, Caixuan, Wang, Yifan, Richard, Audrey S., Raoul, Hervé, Lan, Jiaming, Seeger, Markus A., Cong, Yao, Rockx, Barry, Wong, Gary, Bi, Yuhai, Lavillette, Dimitri, Li, Dianfan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.07.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/326/596/4130; 631/45/535/1266; 82/1; 82/80; 82/83; ACE2; Angiotensin-converting enzyme 2; Angiotensin-Converting Enzyme 2 - metabolism; Animal models; Animals; Antibodies; Antibodies, Neutralizing - immunology; Antibodies, Neutralizing - pharmacology; Antibodies, Neutralizing - ultrastructure; Antibodies, Viral - immunology; Antibodies, Viral - pharmacology; Antibodies, Viral - ultrastructure; Binding; Binding Sites - immunology; Bioavailability; COVID-19; COVID-19 - immunology; COVID-19 - prevention & control; COVID-19 - virology; Cryoelectron Microscopy; Crystallography, X-Ray; Emerging diseases; Female; Hamsters; Human health and pathology; Humanities and Social Sciences; Humans; Immunology; Immunotherapy; Infectious diseases; Life Sciences; Mass Spectrometry - methods; Mesocricetus; Mice; Mice, Inbred C57BL; Microbiology and Parasitology; multidisciplinary; Nanobodies; Neutralization; Neutralization Tests; Neutralizing; Protein Binding - drug effects; Receptors; Receptors, Virus - metabolism; SARS-CoV-2 - immunology; SARS-CoV-2 - metabolism; SARS-CoV-2 - physiology; Science; Science (multidisciplinary); Severe acute respiratory syndrome coronavirus 2; Single-Domain Antibodies - chemistry; Single-Domain Antibodies - immunology; Single-Domain Antibodies - metabolism; Structural analysis; Viral diseases; Virology; Viruses

SARS-CoV-2, the causative agent of COVID-19 1 , features a receptor-binding domain (RBD) for binding to the host cell ACE2 protein 1 – 6 . Neutralizing antibodies that block RBD-ACE2 interaction are candidates for the development of targeted therapeutics 7 – 17 . Llama-derived single-domain antibodi...