Allelic exchange at the endogenous genomic locus in Plasmodium falciparum proves the role of dihydropteroate synthase in sulfadoxine-resistant malaria

• Published in: The EMBO journal Vol. 17; no. 14; pp. 3807 - 3815
• Main Authors: Triglia, Tony, Wang, Ping, Sims, Paul F.G., Hyde, John E., Cowman, Alan F.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 15.07.1998; Nature Publishing Group UK
• Subjects: Alleles; Animals; Antimalarials - pharmacology; Dihydropteroate Synthase - genetics; Dihydropteroate Synthase - physiology; DNA, Protozoan - analysis; drug resistance; Drug Resistance - genetics; Fansidar; Genes, Protozoan - genetics; malaria; Mutagenesis; Mutation - genetics; Plasmodium falciparum - drug effects; Plasmodium falciparum - enzymology; Plasmodium falciparum - genetics; Polyploidy; Recombination, Genetic; RNA, Messenger - analysis; RNA, Protozoan - analysis; sulfadoxine; Sulfadoxine - pharmacology; transfection; sulfadoxine; drug resistance; malaria; Fansidar; transfection

We have exploited the recently developed ability to trans‐ fect the malaria parasite Plasmodium falciparum to investigate the role of polymorphisms in the enzyme dihydropteroate synthase (DHPS), identified in sulfadoxine‐resistant field isolates. By using a truncated form of the dhps gene, specific...