Generating a transgenic mouse line stably expressing human MHC surface antigen from a HAC carrying multiple genomic BACs

• Published in: Chromosoma Vol. 124; no. 1; pp. 107 - 118
• Main Authors: Hasegawa, Yoshinori, Ishikura, Tomoyuki, Hasegawa, Takanori, Watanabe, Takashi, Suzuki, Junpei, Nakayama, Manabu, Okamura, Yoshiaki, Okazaki, Tuneko, Koseki, Haruhiko, Ohara, Osamu, Ikeno, Masashi, Masumoto, Hiroshi
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2015; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Animals; Biochemistry; Biomedical and Life Sciences; Cell Biology; CHO Cells; Chromosomes, Artificial, Bacterial; Chromosomes, Artificial, Human; Cricetulus; Developmental Biology; Eukaryotic Microbiology; Gene Expression Regulation; Genome; HLA-DR Antigens - genetics; Human Genetics; Humans; Life Sciences; Mice; Mice, Transgenic - genetics; Organ Specificity; Research Article; Transgenes; Major Histocompatibility Complex; Chinese Hamster Ovary Cell; Mouse Embryonic Stem Cell; Embryonic Stem Cell; Embryonic Stem Cell Line

The human artificial chromosome (HAC) vector is a promising tool to improve the problematic suppression and position effects of transgene expression frequently seen in transgenic cells and animals produced by conventional plasmid or viral vectors. We generated transgenic mice maintaining a single HA...