Generating a transgenic mouse line stably expressing human MHC surface antigen from a HAC carrying multiple genomic BACs

The human artificial chromosome (HAC) vector is a promising tool to improve the problematic suppression and position effects of transgene expression frequently seen in transgenic cells and animals produced by conventional plasmid or viral vectors. We generated transgenic mice maintaining a single HA...

Full description

Saved in:

Bibliographic Details
Published in	Chromosoma Vol. 124; no. 1; pp. 107 - 118
Main Authors	Hasegawa, Yoshinori, Ishikura, Tomoyuki, Hasegawa, Takanori, Watanabe, Takashi, Suzuki, Junpei, Nakayama, Manabu, Okamura, Yoshiaki, Okazaki, Tuneko, Koseki, Haruhiko, Ohara, Osamu, Ikeno, Masashi, Masumoto, Hiroshi
Format	Journal Article
Language	English
Published	Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2015 Springer Nature B.V
Subjects	Animal Genetics and Genomics Animals Biochemistry Biomedical and Life Sciences Cell Biology CHO Cells Chromosomes, Artificial, Bacterial Chromosomes, Artificial, Human Cricetulus Developmental Biology Eukaryotic Microbiology Gene Expression Regulation Genome HLA-DR Antigens - genetics Human Genetics Humans Life Sciences Mice Mice, Transgenic - genetics Organ Specificity Research Article Transgenes Major Histocompatibility Complex Chinese Hamster Ovary Cell Mouse Embryonic Stem Cell Embryonic Stem Cell Embryonic Stem Cell Line
Online Access	Get full text

Cover

Loading…

Abstract	The human artificial chromosome (HAC) vector is a promising tool to improve the problematic suppression and position effects of transgene expression frequently seen in transgenic cells and animals produced by conventional plasmid or viral vectors. We generated transgenic mice maintaining a single HAC vector carrying two genomic bacterial artificial chromosomes (BACs) from human HLA-DR loci (DRA and DRB1). Both transgenes on the HAC in transgenic mice exhibited tissue-specific expression in kidney, liver, lung, spleen, lymph node, bone marrow, and thymus cells in RT-PCR analysis. Stable functional expression of a cell surface HLA-DR marker from both transgenes, DRA and DRB1 on the HAC, was detected by flow cytometric analysis of splenocytes and maintained through at least eight filial generations. These results indicate that the de novo HAC system can allow us to manipulate multiple BAC transgenes with coordinated expression as a surface antigen through the generation of transgenic animals.
AbstractList	The human artificial chromosome (HAC) vector is a promising tool to improve the problematic suppression and position effects of transgene expression frequently seen in transgenic cells and animals produced by conventional plasmid or viral vectors. We generated transgenic mice maintaining a single HAC vector carrying two genomic bacterial artificial chromosomes (BACs) from human HLA-DR loci (DRA and DRB1). Both transgenes on the HAC in transgenic mice exhibited tissue-specific expression in kidney, liver, lung, spleen, lymph node, bone marrow, and thymus cells in RT-PCR analysis. Stable functional expression of a cell surface HLA-DR marker from both transgenes, DRA and DRB1 on the HAC, was detected by flow cytometric analysis of splenocytes and maintained through at least eight filial generations. These results indicate that the de novo HAC system can allow us to manipulate multiple BAC transgenes with coordinated expression as a surface antigen through the generation of transgenic animals.
Author	Koseki, Haruhiko Suzuki, Junpei Hasegawa, Yoshinori Watanabe, Takashi Okazaki, Tuneko Ishikura, Tomoyuki Hasegawa, Takanori Nakayama, Manabu Masumoto, Hiroshi Ohara, Osamu Ikeno, Masashi Okamura, Yoshiaki
Author_xml	– sequence: 1 givenname: Yoshinori surname: Hasegawa fullname: Hasegawa, Yoshinori organization: Laboratory of Cell Engineering, Department of Frontier Research, Kazusa DNA Research Institute, Department of Technology Development, Kazusa DNA Research Institute – sequence: 2 givenname: Tomoyuki surname: Ishikura fullname: Ishikura, Tomoyuki organization: RIKEN Center for Integrative Medical Sciences (IMS-RCAI) – sequence: 3 givenname: Takanori surname: Hasegawa fullname: Hasegawa, Takanori organization: RIKEN Center for Integrative Medical Sciences (IMS-RCAI) – sequence: 4 givenname: Takashi surname: Watanabe fullname: Watanabe, Takashi organization: RIKEN Center for Integrative Medical Sciences (IMS-RCAI) – sequence: 5 givenname: Junpei surname: Suzuki fullname: Suzuki, Junpei organization: Department of Technology Development, Kazusa DNA Research Institute – sequence: 6 givenname: Manabu surname: Nakayama fullname: Nakayama, Manabu organization: Department of Technology Development, Kazusa DNA Research Institute – sequence: 7 givenname: Yoshiaki surname: Okamura fullname: Okamura, Yoshiaki organization: Laboratory of Cell Engineering, Department of Frontier Research, Kazusa DNA Research Institute – sequence: 8 givenname: Tuneko surname: Okazaki fullname: Okazaki, Tuneko organization: Chromo Research Inc – sequence: 9 givenname: Haruhiko surname: Koseki fullname: Koseki, Haruhiko organization: RIKEN Center for Integrative Medical Sciences (IMS-RCAI) – sequence: 10 givenname: Osamu surname: Ohara fullname: Ohara, Osamu organization: Department of Technology Development, Kazusa DNA Research Institute, RIKEN Center for Integrative Medical Sciences (IMS-RCAI) – sequence: 11 givenname: Masashi surname: Ikeno fullname: Ikeno, Masashi organization: Chromo Research Inc – sequence: 12 givenname: Hiroshi surname: Masumoto fullname: Masumoto, Hiroshi email: masumoto@kazusa.or.jp organization: Laboratory of Cell Engineering, Department of Frontier Research, Kazusa DNA Research Institute
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/25308419$$D View this record in MEDLINE/PubMed
BookMark	eNqNkk1v1DAQhi1URLeFH8AFWeLCJTCOHTu-IC0R7SIVcend8jqTbarEXuyk6v57nG6pChISJ8ueZ9758HtGTnzwSMhbBh8ZgPqUAAQrC2CiAFHXBX9BVkzw_FLX8oSsAEAXlWbVKTlL6Xa5lhJekdOy4lALplfk_hI9Rjv1fkctnaL1aYe-d3QMc0I69B5pmux2OFC830dMaSFv5tF6-n3T0DTHzjqk1k99TqRdDGMW2qwb6myMh4Ue52Hq9wPSDIQxa39ZN-k1ednZIeGbx_OcXF98vW42xdWPy2_N-qpwkuup2ErhKiWYdbnjEgQowZUFaF1bV1q3HEql3FZhrRiICiQCsLZTqEtWl46fk89H2f28HbF16POMg9nHfrTxYILtzZ8R39-YXbgzgnMtNc8CHx4FYvg5Y5rM2CeHw2A95hUZJmVepBSa_Qda1QqEZAv6_i_0NszR50U8UFCVUslMsSPlYkgpYvfUNwOzOMAcHWCyA8ziALP0--75wE8Zv788A-URSDnkdxiflf6n6i_jg7zH
CitedBy_id	crossref_primary_10_1007_s00018_020_03653_1 crossref_primary_10_1021_acssynbio_8b00230 crossref_primary_10_1016_j_yexcr_2020_111914 crossref_primary_10_1007_s10577_014_9459_z crossref_primary_10_1093_biomethods_bpy013 crossref_primary_10_18632_oncotarget_28020 crossref_primary_10_1021_acssynbio_6b00074 crossref_primary_10_1038_mtm_2016_43 crossref_primary_10_1016_j_yexcr_2020_111900 crossref_primary_10_1038_s10038_017_0378_7 crossref_primary_10_1021_acssynbio_7b00209 crossref_primary_10_1016_j_yexcr_2020_111882 crossref_primary_10_1016_j_yexcr_2019_111793
Cites_doi	10.1186/1471-2172-6-24 10.1074/jbc.M603053200 10.1007/s00335-007-9056-y 10.2144/05386RR02 10.1038/nbt0598-431 10.1093/intimm/dxs157 10.1093/intimm/dxs045 10.1538/expanim.61.489 10.1007/s11248-013-9772-x 10.1038/emboj.2012.82 10.1038/mt.2011.136 10.1093/dnares/dsq020 10.1007/BF00191886 10.1007/s11248-010-9469-3 10.1093/nar/gkp058 10.1038/nature02033 10.1093/nar/gks874 10.1038/ng0497-345 10.1016/0198-8859(93)90180-9 10.1128/MCB.01318-09 10.1007/s00335-003-4002-0 10.1093/nar/gkq1280 10.1093/hmg/3.8.1245 10.1093/nar/gkq860 10.1002/j.1460-2075.1985.tb03918.x
ContentType	Journal Article
Copyright	The Author(s) 2014 Springer-Verlag Berlin Heidelberg 2015
Copyright_xml	– notice: The Author(s) 2014 – notice: Springer-Verlag Berlin Heidelberg 2015
DBID	C6C CGR CUY CVF ECM EIF NPM AAYXX CITATION 3V. 7QP 7QR 7TK 7TM 7X7 7XB 88A 88E 8AO 8FD 8FE 8FH 8FI 8FJ 8FK ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ HCIFZ K9. LK8 M0S M1P M7N M7P P64 PQEST PQQKQ PQUKI PRINS RC3 7X8 5PM
DOI	10.1007/s00412-014-0488-3
DatabaseName	Springer Nature OA/Free Journals* Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef ProQuest Central (Corporate) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) ProQuest Pharma Collection Technology Research Database ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Biological Sciences Health & Medical Collection (Alumni Edition) Medical Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Biotechnology and BioEngineering Abstracts ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles)
DatabaseTitle	MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef ProQuest Central Student Technology Research Database ProQuest Central Essentials Nucleic Acids Abstracts ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central China ProQuest Biology Journals (Alumni Edition) ProQuest Central Genetics Abstracts Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Collection Chemoreception Abstracts ProQuest Medical Library (Alumni) ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection Neurosciences Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition Engineering Research Database ProQuest One Academic Calcium & Calcified Tissue Abstracts ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	MEDLINE ProQuest Central Student Genetics Abstracts
Database_xml	– sequence: 1 dbid: C6C name: SpringerOpen url: http://www.springeropen.com/ sourceTypes: Publisher – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Biology
EISSN	1432-0886
EndPage	118
ExternalDocumentID	3603690621 10_1007_s00412_014_0488_3 25308419
Genre	Research Support, Non-U.S. Gov't Journal Article Feature
GroupedDBID	--- -4W -56 -5G -BR -DZ -EM -Y2 -~C -~X .55 .86 .GJ 06C 06D 0R~ 0VY 199 1SB 2.D 203 28- 29B 29~ 2J2 2JN 2JY 2KG 2KM 2LR 2P1 2VQ 2~H 30V 36B 3SX 3V. 4.4 406 408 409 40D 40E 53G 5GY 5QI 5RE 5VS 67N 67Z 6NX 78A 7X7 88A 88E 8AO 8FE 8FH 8FI 8FJ 8TC 8UJ 95- 95. 95~ 96X AAAVM AABHQ AABYN AAFGU AAGAY AAHNG AAIAL AAJKR AANXM AANZL AAPBV AARHV AARTL AATNV AATVU AAUYE AAWCG AAYFA AAYIU AAYQN AAYTO ABBBX ABBXA ABDZT ABECU ABELW ABFGW ABFTV ABHLI ABHQN ABJNI ABJOX ABKAS ABKCH ABKTR ABMNI ABMQK ABNWP ABPLI ABPTK ABQBU ABSXP ABTEG ABTHY ABTKH ABTMW ABULA ABUWG ABWNU ABXPI ACBMV ACBRV ACBXY ACBYP ACGFS ACHSB ACHXU ACIGE ACIPQ ACIWK ACKNC ACMDZ ACMLO ACNCT ACOKC ACOMO ACPRK ACTTH ACVWB ACWMK ADBBV ADHHG ADHIR ADIMF ADINQ ADKNI ADKPE ADMDM ADOAH ADOXG ADRFC ADTPH ADURQ ADYFF ADYPR ADZKW AEBTG AEEQQ AEFIE AEFTE AEGAL AEGNC AEJHL AEJRE AEKMD AENEX AEOHA AEPYU AESKC AESTI AETLH AEVLU AEVTX AEXYK AFEXP AFGCZ AFKRA AFLOW AFMIJ AFNRJ AFQWF AFWTZ AFZKB AGAYW AGDGC AGGBP AGGDS AGJBK AGMZJ AGQMX AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHMBA AHSBF AHYZX AIAKS AIIXL AILAN AIMYW AITGF AJBLW AJDOV AJRNO AJZVZ AKMHD AKQUC ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMXSW AMYLF AMYQR AOCGG AOSHJ ARMRJ ASPBG AVWKF AXYYD AZFZN B-. BA0 BBNVY BBWZM BDATZ BENPR BGNMA BHPHI BPHCQ BVXVI C6C CAG CCPQU COF CS3 CSCUP DDRTE DL5 DNIVK DPUIP DU5 EBD EBLON EBS EIOEI EJD EMB EMOBN EN4 EPAXT ESBYG F5P FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FWDCC FYUFA G-Y G-Z GGCAI GGRSB GJIRD GNWQR GQ6 GQ7 GQ8 GXS HCIFZ HF~ HG5 HG6 HMCUK HMJXF HQYDN HRMNR HVGLF HZ~ I09 IHE IJ- IKXTQ ITM IWAJR IXC IZIGR IZQ I~X I~Z J-C J0Z JBSCW JCJTX JZLTJ KDC KOV KOW KPH LAS LK8 LLZTM M0L M1P M4Y M7P MA- MQGED MVM N2Q N9A NB0 NDZJH NPVJJ NQJWS NU0 O9- O93 O9G O9I O9J OAM OVD P19 PF0 PQQKQ PROAC PSQYO PT4 PT5 Q2X QOK QOR QOS R4E R89 R9I RHV RIG RNI ROL RPX RRX RSV RZK S16 S1Z S26 S27 S28 S3A S3B SAP SBL SBY SCLPG SDH SDM SHX SISQX SJYHP SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW SSXJD STPWE SV3 SZN T13 T16 TEORI TN5 TSG TSK TSV TUC U2A U9L UG4 UKHRP UNUBA UOJIU UTJUX UZXMN VC2 VFIZW W23 W48 WH7 WJK WK6 WK8 X7M XOL Y6R YLTOR Z45 Z7U Z82 Z8O Z8V ZCG ZGI ZMTXR ZOVNA ZXP ~EX AACDK AAEOY AAHBH AAJBT AAQLM AASML AAYZH ABAKF ACAOD ACDTI ACZOJ AEFQL AEMSY AFBBN AGQEE AGRTI AIGIU ALIPV CGR CUY CVF ECM EIF H13 NPM AAYXX ABQSL CITATION 7QP 7QR 7TK 7TM 7XB 8FD 8FK AZQEC DWQXO FR3 GNUQQ K9. M7N P64 PQEST PQUKI PRINS RC3 7X8 5PM
ID	FETCH-LOGICAL-c639t-b64c5741ac30820407437a00dcd8599d30277cb7e87104506e001df7e92182c3
IEDL.DBID	7X7
ISSN	0009-5915
IngestDate	Tue Sep 17 21:06:52 EDT 2024 Wed Dec 04 08:11:27 EST 2024 Wed Dec 04 00:04:09 EST 2024 Thu Oct 10 16:47:15 EDT 2024 Fri Dec 06 01:39:07 EST 2024 Tue Oct 15 23:49:58 EDT 2024 Sat Dec 16 12:20:56 EST 2023
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	1
Keywords	Major Histocompatibility Complex Chinese Hamster Ovary Cell Mouse Embryonic Stem Cell Embryonic Stem Cell Embryonic Stem Cell Line
Language	English
License	Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c639t-b64c5741ac30820407437a00dcd8599d30277cb7e87104506e001df7e92182c3
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
OpenAccessLink	https://doi.org/10.1007/s00412-014-0488-3
PMID	25308419
PQID	1658052676
PQPubID	54059
PageCount	12
ParticipantIDs	pubmedcentral_primary_oai_pubmedcentral_nih_gov_4339693 proquest_miscellaneous_1664196491 proquest_miscellaneous_1658704611 proquest_journals_1658052676 crossref_primary_10_1007_s00412_014_0488_3 pubmed_primary_25308419 springer_journals_10_1007_s00412_014_0488_3
PublicationCentury	2000
PublicationDate	2015-03-01
PublicationDateYYYYMMDD	2015-03-01
PublicationDate_xml	– month: 03 year: 2015 text: 2015-03-01 day: 01
PublicationDecade	2010
PublicationPlace	Berlin/Heidelberg
PublicationPlace_xml	– name: Berlin/Heidelberg – name: Austria – name: Heidelberg
PublicationSubtitle	Biology of the Nucleus
PublicationTitle	Chromosoma
PublicationTitleAbbrev	Chromosoma
PublicationTitleAlternate	Chromosoma
PublicationYear	2015
Publisher	Springer Berlin Heidelberg Springer Nature B.V
Publisher_xml	– name: Springer Berlin Heidelberg – name: Springer Nature B.V
References	Ikeno, Grimes, Okazaki, Nakano, Saitoh, Hoshino, McGill, Cooke, Masumoto (CR10) 1998; 16 Suzuki, Nakayama (CR23) 2011; 39 Chandler, Chandler, Broeckelmann, Hou, Southard-Smith, Mortlock (CR3) 2007; 18 Nakayama, Ohara (CR18) 2005; 38 Ohtsuka, Ogiwara, Miura, Mizutani, Warita, Sato, Imai, Hozumi, Sato, Tanaka, Kimura, Inoko (CR19) 2010; 38 Moro, Cecconi, Martinoli, Dallegno, Giabbai, Degano, Glaichenhaus, Protti, Dellabona, Casorati (CR17) 2005; 6 Ikeno, Masumoto, Okazaki (CR9) 1994; 3 Ikeno, Suzuki, Kamiya, Takahashi, Kudoh, Okazaki (CR12) 2012; 40 Ikeno, Suzuki, Hasegawa, Okazaki (CR11) 2009; 37 Iida, Kim, Kazuki, Hoshiya, Takiguchi, Hayashi, Erliandri, Lee, Samoshkin, Masumoto, Earnshaw, Kouprina, Larionov, Oshimura (CR8) 2010; 17 Ohtsuka, Miura, Sato, Kimura, Inoko, Gurumurthy (CR20) 2012; 61 Harrington, Van Bokkelen, Mays, Gustashaw, Willard (CR7) 1997; 15 Maehara, Takahashi, Saitoh (CR15) 2010; 30 Gong, Zheng, Doughty, Losos, Didkovsky, Schambra, Nowak, Joyner, Leblanc, Hatten, Heintz (CR6) 2003; 425 Asami, Inoue, Terakawa, Egusa, Inoue (CR2) 2011; 20 Miyamoto, Suzuki, Sakai, Asakawa, Okazaki, Kudoh, Ikeno, Shimizu (CR16) 2014; 23 Suzuki, Nishii, Okazaki, Ikeno (CR24) 2006; 281 Suzuki, Takahashi, Katano, Ito, Ito, Harigae, Ishii, Sugamura (CR25) 2012; 24 Kazuki, Oshimura (CR14) 2011; 19 Pinkert, Widera, Cowing, Heber-Katz, Palmiter, Flavell, Brinster (CR22) 1985; 4 Fukui, Esaki, Kimura, Hirokawa, Nishimura, Sasazuki (CR5) 1993; 37 Ohzeki, Bergmann, Kouprina, Noskov, Nakano, Kimura, Earnshaw, Larionov, Masumoto (CR21) 2012; 31 Vintersten, Testa, Naumann, Anastassiadis, Stewart (CR26) 2008; 415 Jux, Staratschek-Jox, Penninger, Schultze, Kolanus (CR13) 2013; 25 Altmann, Takács, Trowsdale, Elliott (CR1) 1993; 37 Doherty, Fisher (CR4) 2003; 14 9090378 - Nat Genet. 1997 Apr;15(4):345-55 21750534 - Mol Ther. 2011 Sep;19(9):1591-601 20880997 - Nucleic Acids Res. 2010 Dec;38(22):e198 23095812 - Exp Anim. 2012;61(5):489-502 21132362 - Transgenic Res. 2011 Aug;20(4):913-24 23002136 - Nucleic Acids Res. 2012 Nov;40(21):10742-52 19223328 - Nucleic Acids Res. 2009 Apr;37(6):e44 16018553 - Biotechniques. 2005 Jun;38(6):917-24 17882484 - Mamm Genome. 2007 Oct;18(10):693-708 14629108 - Mamm Genome. 2003 Sep;14(9):583-92 3935430 - EMBO J. 1985 Sep;4(9):2225-30 18370149 - Methods Mol Biol. 2008;415:83-100 14586460 - Nature. 2003 Oct 30;425(6961):917-25 22473132 - EMBO J. 2012 May 16;31(10):2391-402 9592390 - Nat Biotechnol. 1998 May;16(5):431-9 16329759 - BMC Immunol. 2005;6:24 16837455 - J Biol Chem. 2006 Sep 8;281(36):26615-23 22402880 - Int Immunol. 2012 Apr;24(4):243-52 23391492 - Int Immunol. 2013 May;25(5):307-17 24293126 - Transgenic Res. 2014 Apr;23(2):317-29 20160010 - Mol Cell Biol. 2010 May;30(9):2090-104 21288882 - Nucleic Acids Res. 2011 Apr;39(8):e49 8244778 - Hum Immunol. 1993 Jul;37(3):149-56 7987298 - Hum Mol Genet. 1994 Aug;3(8):1245-57 20798231 - DNA Res. 2010 Oct;17(5):293-301 8420827 - Immunogenetics. 1993;37(3):204-11 K Vintersten (488_CR26) 2008; 415 M Ohtsuka (488_CR20) 2012; 61 J Ohzeki (488_CR21) 2012; 31 JJ Harrington (488_CR7) 1997; 15 B Jux (488_CR13) 2013; 25 M Nakayama (488_CR18) 2005; 38 K Maehara (488_CR15) 2010; 30 K Miyamoto (488_CR16) 2014; 23 M Ikeno (488_CR10) 1998; 16 J Asami (488_CR2) 2011; 20 M Moro (488_CR17) 2005; 6 AM Doherty (488_CR4) 2003; 14 CA Pinkert (488_CR22) 1985; 4 S Gong (488_CR6) 2003; 425 M Ikeno (488_CR9) 1994; 3 N Suzuki (488_CR24) 2006; 281 KJ Chandler (488_CR3) 2007; 18 Y Fukui (488_CR5) 1993; 37 DM Altmann (488_CR1) 1993; 37 M Ikeno (488_CR12) 2012; 40 M Ohtsuka (488_CR19) 2010; 38 M Ikeno (488_CR11) 2009; 37 E Suzuki (488_CR23) 2011; 39 Y Kazuki (488_CR14) 2011; 19 Y Iida (488_CR8) 2010; 17 M Suzuki (488_CR25) 2012; 24
References_xml	– volume: 6 start-page: 24 year: 2005 ident: CR17 article-title: Generation of functional HLA-DR*1101 tetramers receptive for loading with pathogen or tumour derived synthetic peptides publication-title: BMC Immunol doi: 10.1186/1471-2172-6-24 contributor: fullname: Casorati – volume: 281 start-page: 26615 year: 2006 end-page: 26623 ident: CR24 article-title: Human artificial chromosomes constructed using the bottom-up strategy are stably maintained in mitosis and efficiently transmissible to progeny mice publication-title: J Biol Chem doi: 10.1074/jbc.M603053200 contributor: fullname: Ikeno – volume: 18 start-page: 693 year: 2007 end-page: 708 ident: CR3 article-title: Relevance of BAC transgene copy number in mice: transgene copy number variation across multiple transgenic lines and correlations with transgene integrity and expression publication-title: Mamm Genome doi: 10.1007/s00335-007-9056-y contributor: fullname: Mortlock – volume: 38 start-page: 917 year: 2005 end-page: 924 ident: CR18 article-title: Improvement of recombination efficiency by mutation of Red proteins publication-title: Biotechniques doi: 10.2144/05386RR02 contributor: fullname: Ohara – volume: 4 start-page: 2225 year: 1985 end-page: 2230 ident: CR22 article-title: Tissue-specific, inducible and functional expression of the E alpha d MHC class II gene in transgenic mice publication-title: EMBO J contributor: fullname: Brinster – volume: 16 start-page: 431 year: 1998 end-page: 439 ident: CR10 article-title: Construction of YAC-based mammalian artificial chromosomes publication-title: Nat Biotechnol doi: 10.1038/nbt0598-431 contributor: fullname: Masumoto – volume: 25 start-page: 307 year: 2013 end-page: 317 ident: CR13 article-title: Vav1 regulates MHCII expression in murine resting and activated B cells publication-title: Int Immunol doi: 10.1093/intimm/dxs157 contributor: fullname: Kolanus – volume: 24 start-page: 243 year: 2012 end-page: 252 ident: CR25 article-title: Induction of human humoral immune responses in a novel HLA-DR-expressing transgenic NOD/Shi-scid/γcnull mouse publication-title: Int Immunol doi: 10.1093/intimm/dxs045 contributor: fullname: Sugamura – volume: 61 start-page: 489 year: 2012 end-page: 502 ident: CR20 article-title: PITT: pronuclear injection-based targeted transgenesis, a reliable transgene expression method in mice publication-title: Exp Anim doi: 10.1538/expanim.61.489 contributor: fullname: Gurumurthy – volume: 23 start-page: 317 year: 2014 end-page: 329 ident: CR16 article-title: A novel mouse model for Down syndrome that harbor a single copy of human artificial chromosome (HAC) carrying a limited number of genes from human chromosome 21 publication-title: Transgenic Res doi: 10.1007/s11248-013-9772-x contributor: fullname: Shimizu – volume: 31 start-page: 2391 year: 2012 end-page: 2402 ident: CR21 article-title: Breaking the HAC barrier: histone H3K9 acetyl/methyl balance regulates CENP-A assembly publication-title: EMBO J doi: 10.1038/emboj.2012.82 contributor: fullname: Masumoto – volume: 19 start-page: 1591 year: 2011 end-page: 1601 ident: CR14 article-title: Human artificial chromosomes for gene delivery and the development of animal models publication-title: Mol Ther doi: 10.1038/mt.2011.136 contributor: fullname: Oshimura – volume: 17 start-page: 293 year: 2010 end-page: 301 ident: CR8 article-title: Human artificial chromosome with a conditional centromere for gene delivery and gene expression publication-title: DNA Res doi: 10.1093/dnares/dsq020 contributor: fullname: Oshimura – volume: 37 start-page: 204 year: 1993 end-page: 211 ident: CR5 article-title: T-cell repertoire in a strain of transgenic C57BL/6 mice with the HLA-DRA gene on the X-chromosome publication-title: Immunogenetics doi: 10.1007/BF00191886 contributor: fullname: Sasazuki – volume: 20 start-page: 913 year: 2011 end-page: 924 ident: CR2 article-title: Bacterial artificial chromosomes as analytical basis for gene transcriptional machineries publication-title: Transgenic Res doi: 10.1007/s11248-010-9469-3 contributor: fullname: Inoue – volume: 37 start-page: e44 year: 2009 ident: CR11 article-title: Manipulating transgenes using a chromosome vector publication-title: Nucleic Acids Res doi: 10.1093/nar/gkp058 contributor: fullname: Okazaki – volume: 425 start-page: 917 year: 2003 end-page: 925 ident: CR6 article-title: A gene expression atlas of the central nervous system based on bacterial artificial chromosomes publication-title: Nature doi: 10.1038/nature02033 contributor: fullname: Heintz – volume: 40 start-page: 10742 year: 2012 end-page: 10752 ident: CR12 article-title: LINE1 family member is negative regulator of HLA-G expression publication-title: Nucleic Acids Res doi: 10.1093/nar/gks874 contributor: fullname: Okazaki – volume: 15 start-page: 345 year: 1997 end-page: 355 ident: CR7 article-title: Formation of de novo centromeres and construction of first-generation human artificial microchromosomes publication-title: Nat Genet doi: 10.1038/ng0497-345 contributor: fullname: Willard – volume: 37 start-page: 149 year: 1993 end-page: 156 ident: CR1 article-title: Mouse mammary tumor virus-mediated T-cell receptor negative selection in HLA-DRA transgenic mice publication-title: Hum Immunol doi: 10.1016/0198-8859(93)90180-9 contributor: fullname: Elliott – volume: 30 start-page: 2090 year: 2010 end-page: 2104 ident: CR15 article-title: CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses publication-title: Mol Cell Biol doi: 10.1128/MCB.01318-09 contributor: fullname: Saitoh – volume: 14 start-page: 583 year: 2003 end-page: 592 ident: CR4 article-title: Microcell-mediated chromosome transfer (MMCT): small cells with huge potential publication-title: Mamm Genome doi: 10.1007/s00335-003-4002-0 contributor: fullname: Fisher – volume: 39 start-page: e49 year: 2011 ident: CR23 article-title: VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering publication-title: Nucleic Acids Res doi: 10.1093/nar/gkq1280 contributor: fullname: Nakayama – volume: 3 start-page: 1245 year: 1994 end-page: 1257 ident: CR9 article-title: Distribution of CENP-B boxes reflected in CREST centromere antigenic sites on long-range alpha-satellite DNA arrays of human chromosome 21 publication-title: Hum Mol Genet doi: 10.1093/hmg/3.8.1245 contributor: fullname: Okazaki – volume: 415 start-page: 83 year: 2008 end-page: 100 ident: CR26 article-title: Bacterial artificial chromosome transgenesis through pronuclear injection of fertilized mouse oocytes publication-title: Methods Mol Biol contributor: fullname: Stewart – volume: 38 start-page: e198 year: 2010 ident: CR19 article-title: Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression publication-title: Nucleic Acids Res doi: 10.1093/nar/gkq860 contributor: fullname: Inoko – volume: 281 start-page: 26615 year: 2006 ident: 488_CR24 publication-title: J Biol Chem doi: 10.1074/jbc.M603053200 contributor: fullname: N Suzuki – volume: 6 start-page: 24 year: 2005 ident: 488_CR17 publication-title: BMC Immunol doi: 10.1186/1471-2172-6-24 contributor: fullname: M Moro – volume: 425 start-page: 917 year: 2003 ident: 488_CR6 publication-title: Nature doi: 10.1038/nature02033 contributor: fullname: S Gong – volume: 16 start-page: 431 year: 1998 ident: 488_CR10 publication-title: Nat Biotechnol doi: 10.1038/nbt0598-431 contributor: fullname: M Ikeno – volume: 20 start-page: 913 year: 2011 ident: 488_CR2 publication-title: Transgenic Res doi: 10.1007/s11248-010-9469-3 contributor: fullname: J Asami – volume: 37 start-page: e44 year: 2009 ident: 488_CR11 publication-title: Nucleic Acids Res doi: 10.1093/nar/gkp058 contributor: fullname: M Ikeno – volume: 24 start-page: 243 year: 2012 ident: 488_CR25 publication-title: Int Immunol doi: 10.1093/intimm/dxs045 contributor: fullname: M Suzuki – volume: 61 start-page: 489 year: 2012 ident: 488_CR20 publication-title: Exp Anim doi: 10.1538/expanim.61.489 contributor: fullname: M Ohtsuka – volume: 40 start-page: 10742 year: 2012 ident: 488_CR12 publication-title: Nucleic Acids Res doi: 10.1093/nar/gks874 contributor: fullname: M Ikeno – volume: 415 start-page: 83 year: 2008 ident: 488_CR26 publication-title: Methods Mol Biol contributor: fullname: K Vintersten – volume: 38 start-page: 917 year: 2005 ident: 488_CR18 publication-title: Biotechniques doi: 10.2144/05386RR02 contributor: fullname: M Nakayama – volume: 18 start-page: 693 year: 2007 ident: 488_CR3 publication-title: Mamm Genome doi: 10.1007/s00335-007-9056-y contributor: fullname: KJ Chandler – volume: 37 start-page: 204 year: 1993 ident: 488_CR5 publication-title: Immunogenetics doi: 10.1007/BF00191886 contributor: fullname: Y Fukui – volume: 30 start-page: 2090 year: 2010 ident: 488_CR15 publication-title: Mol Cell Biol doi: 10.1128/MCB.01318-09 contributor: fullname: K Maehara – volume: 4 start-page: 2225 year: 1985 ident: 488_CR22 publication-title: EMBO J doi: 10.1002/j.1460-2075.1985.tb03918.x contributor: fullname: CA Pinkert – volume: 15 start-page: 345 year: 1997 ident: 488_CR7 publication-title: Nat Genet doi: 10.1038/ng0497-345 contributor: fullname: JJ Harrington – volume: 23 start-page: 317 year: 2014 ident: 488_CR16 publication-title: Transgenic Res doi: 10.1007/s11248-013-9772-x contributor: fullname: K Miyamoto – volume: 19 start-page: 1591 year: 2011 ident: 488_CR14 publication-title: Mol Ther doi: 10.1038/mt.2011.136 contributor: fullname: Y Kazuki – volume: 14 start-page: 583 year: 2003 ident: 488_CR4 publication-title: Mamm Genome doi: 10.1007/s00335-003-4002-0 contributor: fullname: AM Doherty – volume: 39 start-page: e49 year: 2011 ident: 488_CR23 publication-title: Nucleic Acids Res doi: 10.1093/nar/gkq1280 contributor: fullname: E Suzuki – volume: 31 start-page: 2391 year: 2012 ident: 488_CR21 publication-title: EMBO J doi: 10.1038/emboj.2012.82 contributor: fullname: J Ohzeki – volume: 17 start-page: 293 year: 2010 ident: 488_CR8 publication-title: DNA Res doi: 10.1093/dnares/dsq020 contributor: fullname: Y Iida – volume: 37 start-page: 149 year: 1993 ident: 488_CR1 publication-title: Hum Immunol doi: 10.1016/0198-8859(93)90180-9 contributor: fullname: DM Altmann – volume: 25 start-page: 307 year: 2013 ident: 488_CR13 publication-title: Int Immunol doi: 10.1093/intimm/dxs157 contributor: fullname: B Jux – volume: 38 start-page: e198 year: 2010 ident: 488_CR19 publication-title: Nucleic Acids Res doi: 10.1093/nar/gkq860 contributor: fullname: M Ohtsuka – volume: 3 start-page: 1245 year: 1994 ident: 488_CR9 publication-title: Hum Mol Genet doi: 10.1093/hmg/3.8.1245 contributor: fullname: M Ikeno
SSID	ssj0009260
Score	2.2179444
Snippet	The human artificial chromosome (HAC) vector is a promising tool to improve the problematic suppression and position effects of transgene expression frequently...
SourceID	pubmedcentral proquest crossref pubmed springer
SourceType	Open Access Repository Aggregation Database Index Database Publisher
StartPage	107
SubjectTerms	Animal Genetics and Genomics Animals Biochemistry Biomedical and Life Sciences Cell Biology CHO Cells Chromosomes, Artificial, Bacterial Chromosomes, Artificial, Human Cricetulus Developmental Biology Eukaryotic Microbiology Gene Expression Regulation Genome HLA-DR Antigens - genetics Human Genetics Humans Life Sciences Mice Mice, Transgenic - genetics Organ Specificity Research Article Transgenes
SummonAdditionalLinks	– databaseName: Springer Nature OA/Free Journals* dbid: C6C link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1LS8NAEF60IngR30arrOBJCeSx2STHGpQg1FOF3sLuZoMFjZKm0P57Z_LStip43mHZzDdhvmVmviXkWgtXCivjJk9TDy4onJvSUdLMINtxO9CWkDjgPHzi8TN7HHvjRiwaZ2FW6veV2GfVPIDtEgCqu0m2IAX7GMwRj770dR1utY-meaHttQXMn7ZYTkFrvHK9PXKlRlqlnoc9sttwRjqoQd4nGzo_INv1K5KLQzKvpaOxf5kKWmLygaiYKIq3ek2RR1LggPJ1QfW87nsFy-pxPjqMIzqdFZlQmoKPUZqT4sQJbBQPIqpEUeAcFG37Dilqur7B3neDaHpERg_3oyg2mwcVTAVEpDQlZ8oDCiEUitTA7wv0wReWlao08MIwxRqmr6Sv4RYFVM_iGpJYmvk6RJ135R6TXv6e61NCuZ0GKVOZdlnGmAgCwNuxJdyNmAa6bhnkpnVx8lHLZiSdQHKFRwJ4JIhH4hqk34KQNH_QNLEhWFCLxucGueqWIfaxoCFyDR6sbHxUjLf_suEMRcdCsDmpce1O5HjgB1g1iL-EeGeA2tvLK_nkpdLgZq4b8hCOftvGxrej__ahZ_-yPic7wM68uuGtT3plMdMXwIBKeVnF_icauvyX priority: 102 providerName: Springer Nature
Title	Generating a transgenic mouse line stably expressing human MHC surface antigen from a HAC carrying multiple genomic BACs
URI	https://link.springer.com/article/10.1007/s00412-014-0488-3 https://www.ncbi.nlm.nih.gov/pubmed/25308419 https://www.proquest.com/docview/1658052676 https://search.proquest.com/docview/1658704611 https://search.proquest.com/docview/1664196491 https://pubmed.ncbi.nlm.nih.gov/PMC4339693
Volume	124
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1bS9xAFB6sUuhL0V5XrUyhTy2huUxmkqcSw9rFopSisH0KcwsV2mh3V9B_73dyWV1FXxLInITJnDM53-Sc-Q5jn7xOjA5rGUjnUixQpAxMbE1Qw9vJKPOhNrTB-ehYTk7F4TSd9j_c5n1a5fBNbD_U7tzSP_KvEW4mbhIlv138D6hqFEVX-xIaz9hGFMOVw57VVN2S7sYyHCqppXmUDlHNsCMRbZMSKA0DxpKs-qUHYPNhzuS9wGnrjw422cseSPKi0_wWW_PNK_a8Ky15_ZpddXzSlNTMNV-QR4KpnFlOS33PCVxyAEPz95r7qy4ZFpJtxT5-NCn5_HJWa-s5Bp74OjltQ8GDJkXJrZ7NaHMUH5IRORG9_sOz94ty_oadHIxPyknQV1kILNDJIjBS2BS4QltirsGcBqZQOgyddVma544Cm8oa5bG0Av4LpYdnc7XyOZG_2-QtW2_OG_-ecRm5zAlb-0TUQugsgxHEkcGCSXhg-HDEPg9DXF10XBrVkjW51UcFfVSkjyoZsd1BCVU_rebVrRGM2MdlMyYERTl04zGCrYwiGvnoKRkpiIksh8y7Tq_LHsUpxgGtI6ZWNL4UIELu1Zbm7E9LzC2SJJc5uv5lsI07XX_sRbefftEd9gIYLe3S3nbZ-mJ26T8ABy3MXmvse2yj-P77xxjn_fHxz1-4WsoSx9O4uAFTXglC
link.rule.ids	230,314,780,784,885,12056,21388,27924,27925,31719,31720,33744,33745,41081,41120,41523,42150,42189,42592,43310,43805,51576,52111,52234,73745,74302
linkProvider	ProQuest
linkToHtml	http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3dT9swED-NIsReJr62dStgJJ6GoiWN7SRPqFSgDGiFUCfxFvkrotIWWFsk-t_vLh-FguDZF8v2nX0_586_Azh0KtTKz6UnrRV4QZHS012jvRy9nQxi5ytND5wHQ5n-5uc34qb-4Tat0yqbM7E8qO2doX_kPwP8mLhJInl8_8-jqlEUXa1LaKzAKjGnixasnpwOr66faHe70m9qqYkkEE1c069oRMu0BErEQHMJlz3TK7j5OmvyRei09EhnG_CphpKsV-l-Ez64YgvWquKS8214rBilKa2ZKTYjn4TGMjaMLvuOEbxkCA31nzlzj1U6LEqWNfvYIO2z6cMkV8YxXHpi7GT0EAU7Snt9ZtRkQs-jWJOOyIjq9S_2fdLrT3dgdHY66qdeXWfBM4hPZp6W3AhEFsoQdw3uakQVkfJ9a2wsksRSaDMyOnJ4uUIE6EuHvs3mkUuI_t2En6FV3BXuKzAZ2Nhyk7uQ55yrOEYz6AYar0zcIYr32_CjWeLsvmLTyBa8yaU-MtRHRvrIwjZ0GiVk9caaZk9m0IaDRTNuCYpzqMLhCpYyERHJB-_JSE5cZAnKfKn0uhhRV-A6YGsboiWNLwSIknu5pRjfltTcPAwTmeDQjxrbeDb0tyb67f2J7sN6OhpcZpe_hhff4SMiNlElwXWgNZs8uF1ERTO9V5v-f2yOCKQ
linkToPdf	http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LT9tAEB7RoFZcENAWwnORemplsbbXa_uEQiBKC0SoohI3a18WSGAgDyn8e2b8CKQIzjux1juznm8z334D8MOpUCueS09aG-EBRUpPB0Z7OWY76SeOK00XnM8Hsv9P_LmKrmr-06imVTbfxPJDbe8N_Ud-4OOPSZsklgd5TYu4OO4dPjx61EGKKq11O41PsIhZkQctWDw6GVz8fZHgDSRv-qpFqR81NU5eSYqWFAUiZWDohPNZ6g30fMug_K-MWman3gos17CSdao4WIUFV6zB56rR5NNXmFbq0kRxZoqNKT9h4NwYRgd_xwhqMoSJ-vaJuWlFjUXLsn8fO-932WgyzJVxDN1A6p2MLqXgg_qdLjNqOKSrUqyhJjKSfb3DZx91uqNvcNk7uez2vbrngmcQq4w9LYWJEGUoQzo2uMNxLWPFuTU2idLUUpkzNjp2eNBCNMilwzxn89ilJAVvwu_QKu4LtwFM-jaxwuQuFLkQKkkwJAJf4_FJOET0vA0_myXOHipljWymoVz6I0N_ZOSPLGzDduOErN5ko-wlJNqwPxvG7UE1D1U4XMHSJiZRef8jGylIlyxFm_XKr7MZBRGuA462IZ7z-MyA5LnnR4qb61KmW4RhKlOc-q8mNl5N_b0X3fz4RffgC0Z9dvZ7cLoFSwjeoooPtw2t8XDidhAgjfVuHfnP7X8M0Q
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Generating+a+transgenic+mouse+line+stably+expressing+human+MHC+surface+antigen+from+a+HAC+carrying+multiple+genomic+BACs&rft.jtitle=Chromosoma&rft.au=Hasegawa%2C+Yoshinori&rft.au=Ishikura%2C+Tomoyuki&rft.au=Hasegawa%2C+Takanori&rft.au=Watanabe%2C+Takashi&rft.date=2015-03-01&rft.issn=0009-5915&rft.eissn=1432-0886&rft.volume=124&rft.issue=1&rft.spage=107&rft.epage=118&rft_id=info:doi/10.1007%2Fs00412-014-0488-3&rft.externalDBID=n%2Fa&rft.externalDocID=10_1007_s00412_014_0488_3
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0009-5915&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0009-5915&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0009-5915&client=summon