Curcumae Ameliorates Diethylnitrosamine-Induced Hepatocellular Carcinoma via Alteration of Oxidative Stress, Inflammation and Gut Microbiota

• Published in: Journal of inflammation research Vol. 14; pp. 5551 - 5566
• Main Authors: Zhang, Yunyan, Li, Xuelian, Li, Xinghua
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2021; Taylor & Francis Ltd; Dove; Dove Medical Press
• Subjects: Animal experimentation; antioxidant; Antioxidants; Bioengineering; Carcinogens; Chemotherapy; curcumae; Cyclooxygenase-2; Cytochrome; Cytokines; Diethylnitrosamine; Disease; Enzymes; Fatty liver; gut microbiota; hepatic cellular carcinoma; Hepatocellular carcinoma; Hepatoma; High fat diet; IL-1β; Inflammation; Interleukin 10; Interleukin 2; Interleukin 6; Interleukin 7; Interleukins; Intestinal microflora; Liver cancer; Liver cirrhosis; Liver diseases; Manufacturers; Medical prognosis; Microbiota; Microbiota (Symbiotic organisms); Mortality; NF-κB protein; Original Research; Oxidative stress; Phosphatase; Prostaglandin E2; Prostaglandins E; Risk factors; Tumor necrosis factor-α; Variance analysis; China; St Louis Missouri; United States > US; curcumae; antioxidant; inflammation; gut microbiota; hepatic cellular carcinoma
• ISSN: 1178-7031; 1178-7031
• DOI: 10.2147/jir.s330499

Nonalcoholic fatty liver disease (NAFLD) increased the risk factor of hepatocellular carcinoma (HCC). NAFLD induces the hepatic-related cancer deaths mostly in middle-aged men. NAFLD enhanced the inflammatory reaction and oxidative stress in the hepatic tissue. Curcumae exhibited the anti-inflammatory and antioxidant effects. In this study, we made an attempt to scrutinize the protective effect of curcumae on obesity-induced HCC via alteration of inflammation, oxidative stress and gut microbiota. The rats used in this experiment were Wistar rats, 100 mg/kg intraperitoneal injection of diethylnitrosamine (hepatic carcinogen) was used at 2 weeks. After 6 weeks of the experimental study, the rats were randomly divided into high-fat diet (HFD) with or without curcumae-treated group rats and received the treatment for 22 weeks. Hepatic, non-hepatic, cardiac, antioxidant, pro-inflammatory and inflammatory were estimated at the end of the study. The stools of the experimental rats were collected for estimating the gut microbiota. Curcumae-treated group rats exposed reduction of the hepatic nodules in hepatic tissue. Curcumae significantly (P<0.001) diminished the level of hepatic parameters and antioxidant parameters in the serum. Curcumae significantly (P<0.001) suppressed the pro-inflammatory cytokines level, viz. interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), interleukin-7 (IL-7) and augmented the level of interleukin-10 (IL-10) in the serum and hepatic tissue. Curcumae significantly (P<0.001) suppressed inflammatory mediators including cyclooxygenase (COX-2), prostaglandin E2 (PGE2) and nuclear factor kappa B (NF-κB) in the serum and hepatic tissue. Furthermore, curcumae increased the gut microbial diversity and richness and decreased the relative abundance of genus and , respectively. Curcumae prevents HFD-induced inflammation during the hepatic carcinoma by modulating the oxidative stress, inflammatory reaction and gut microbiota.