A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells

• Published in: Biotechnology Letters Vol. 43; no. 6; pp. 1241 - 1251
• Main Authors: Namitha, Bhuvaneshwari, Chitra, Munusamy Rajendran, Bhavya, Mathevan, Parikumar, Periasamy, Katoh, Shojiro, Yoshioka, Hiroshi, Iwasaki, Masaru, Senthilkumar, Rajappa, Rajmohan, Mathaiyan, Karthick, Ramalingam, Preethy, Senthilkumar, Abraham, Samuel J. K.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Science and Business Media LLC 01.06.2021; Springer Netherlands; Springer Nature B.V
• Subjects: Adult; Aged; Aged, 80 and over; Applied Microbiology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cadaver; Cell culture; Cell density; cell growth; Chondroitin Sulfates; Complex Mixtures; Cornea; Culture Media; Density; Dextrans; Endothelial cells; Endothelium, Corneal; Epithelial cells; Epithelium; Female; Gelation; Gentamicins; Humans; Life Sciences; Maintenance; Male; Microbiology; microscopy; Middle Aged; Organic Chemicals; Original Research Paper; phenotype; Phenotypes; Polymerase chain reaction; Polymers; Preservation; Scaffolds; Slit Lamp Microscopy; Stem cells; Tissue Preservation; Transportation; Optisol—GS; Corneal storage; MK medium; TGP; Corneal limbal cell culture

Purpose McCarey-Kaufman’s (MK) medium and Optisol-GS medium are the most commonly employed media for human donor corneal preservation. In this study, we evaluated the preservation efficacy of discarded human donor corneas using a Thermo-reversible gelation polymer (TGP) added to these two media. Met...