Organization of the Influenza Virus Replication Machinery

• Published in: Science (American Association for the Advancement of Science) Vol. 338; no. 6114; pp. 1631 - 1634
• Main Authors: Moeller, Arne, Kirchdoerfer, Robert N., Potter, Clinton S., Carragher, Bridget, Wilson, Ian A.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 21.12.2012; The American Association for the Advancement of Science
• Subjects: Active sites; Assembly; Biological and medical sciences; cryo-electron microscopy; Cryoelectron Microscopy; Crystallography, X-Ray; Electron microscopy; genome; Genome, Viral; Genomes; Genomics; Image Processing, Computer-Assisted; Influenza; Influenza A virus; Influenza A Virus, H1N1 Subtype - chemistry; Influenza A Virus, H1N1 Subtype - genetics; Influenza A Virus, H1N1 Subtype - physiology; Influenza A Virus, H1N1 Subtype - ultrastructure; Influenza virus; Medical sciences; Microscopy; Microscopy, Electron; Models, Molecular; morbidity; Mortality; Nucleic Acid Conformation; Nucleoproteins; Online; Orthomyxoviridae; Pharmacology. Drug treatments; Protein Conformation; Protein Subunits - chemistry; Protein Subunits - metabolism; Replication; Ribonucleic acids; ribonucleoproteins; Ribonucleoproteins - chemistry; Ribonucleoproteins - genetics; Ribonucleoproteins - metabolism; Ribonucleoproteins - ultrastructure; RNA; RNA polymerase; RNA Replicase - chemistry; RNA Replicase - metabolism; RNA Replicase - ultrastructure; RNA, Viral - chemistry; RNA, Viral - metabolism; RNA, Viral - ultrastructure; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - metabolism; RNA-Binding Proteins - ultrastructure; RNA-protein interactions; transcription (genetics); Transcription, Genetic; Viral Core Proteins - chemistry; Viral Core Proteins - metabolism; Viral Core Proteins - ultrastructure; Viral genomes; Viral morphology; Viral Proteins - chemistry; Viral Proteins - metabolism; Viral Proteins - ultrastructure; Virions; Virology; Virus Replication; Viruses; Virus; Anatomic pathology; Ribonucleoprotein; Orthomyxoviridae; Virus replication cycle; Exploration; Replication; Electron microscopy; Influenzavirus

Influenza virus ribonucleoprotein complexes (RNPs) are central to the viral life cycle and in adaptation to new host species. RNPs are composed of the viral genome, viral polymerase, and many copies of the viral nucleoprotein. In vitro cell expression of all RNP protein components with four of the e...