A novel heteromeric pantothenate kinase complex in apicomplexan parasites

Coenzyme A is synthesised from pantothenate via five enzyme-mediated steps. The first step is catalysed by pantothenate kinase (PanK). All PanKs characterised to date form homodimers. Many organisms express multiple PanKs. In some cases, these PanKs are not functionally redundant, and some appear to...

Full description

Saved in:
Bibliographic Details
Published inPLoS pathogens Vol. 17; no. 7; p. e1009797
Main Authors Tjhin, Erick T, Howieson, Vanessa M, Spry, Christina, van Dooren, Giel G, Saliba, Kevin J
Format Journal Article
LanguageEnglish
Published San Francisco Public Library of Science 29.07.2021
Public Library of Science (PLoS)
Subjects
Analysis
Biology and Life Sciences
Chemical properties
Coenzyme A
Coenzymes
Enzyme kinetics
Experiments
Genomes
Homology
Kinases
Medicine and Health Sciences
Mutation
Organisms
Pantothenate kinase
Parasites
Peptides
Phosphorylation
Plasmodium falciparum
Proteins
Research and Analysis Methods
Toxoplasma
Australia
Online AccessGet full text

Cover

Abstract Coenzyme A is synthesised from pantothenate via five enzyme-mediated steps. The first step is catalysed by pantothenate kinase (PanK). All PanKs characterised to date form homodimers. Many organisms express multiple PanKs. In some cases, these PanKs are not functionally redundant, and some appear to be non-functional. Here, we investigate the PanKs in two pathogenic apicomplexan parasites, Plasmodium falciparum and Toxoplasma gondii. Each of these organisms express two PanK homologues (PanK1 and PanK2). We demonstrate that PfPanK1 and PfPanK2 associate, forming a single, functional PanK complex that includes the multi-functional protein, Pf14-3-3I. Similarly, we demonstrate that TgPanK1 and TgPanK2 form a single complex that possesses PanK activity. Both TgPanK1 and TgPanK2 are essential for T. gondii proliferation, specifically due to their PanK activity. Our study constitutes the first examples of heteromeric PanK complexes in nature and provides an explanation for the presence of multiple PanKs within certain organisms.
AbstractList Coenzyme A is synthesised from pantothenate via five enzyme-mediated steps. The first step is catalysed by pantothenate kinase (PanK). All PanKs characterised to date form homodimers. Many organisms express multiple PanKs. In some cases, these PanKs are not functionally redundant, and some appear to be non-functional. Here, we investigate the PanKs in two pathogenic apicomplexan parasites, Plasmodium falciparum and Toxoplasma gondii. Each of these organisms express two PanK homologues (PanK1 and PanK2). We demonstrate that PfPanK1 and PfPanK2 associate, forming a single, functional PanK complex that includes the multi-functional protein, Pf14-3-3I. Similarly, we demonstrate that TgPanK1 and TgPanK2 form a single complex that possesses PanK activity. Both TgPanK1 and TgPanK2 are essential for T. gondii proliferation, specifically due to their PanK activity. Our study constitutes the first examples of heteromeric PanK complexes in nature and provides an explanation for the presence of multiple PanKs within certain organisms.
Coenzyme A is synthesised from pantothenate via five enzyme-mediated steps. The first step is catalysed by pantothenate kinase (PanK). All PanKs characterised to date form homodimers. Many organisms express multiple PanKs. In some cases, these PanKs are not functionally redundant, and some appear to be non-functional. Here, we investigate the PanKs in two pathogenic apicomplexan parasites, Plasmodium falciparum and Toxoplasma gondii . Each of these organisms express two PanK homologues (PanK1 and PanK2). We demonstrate that Pf PanK1 and Pf PanK2 associate, forming a single, functional PanK complex that includes the multi-functional protein, Pf 14-3-3I. Similarly, we demonstrate that Tg PanK1 and Tg PanK2 form a single complex that possesses PanK activity. Both Tg PanK1 and Tg PanK2 are essential for T . gondii proliferation, specifically due to their PanK activity. Our study constitutes the first examples of heteromeric PanK complexes in nature and provides an explanation for the presence of multiple PanKs within certain organisms.
Coenzyme A is synthesised from pantothenate via five enzyme-mediated steps. The first step is catalysed by pantothenate kinase (PanK). All PanKs characterised to date form homodimers. Many organisms express multiple PanKs. In some cases, these PanKs are not functionally redundant, and some appear to be non-functional. Here, we investigate the PanKs in two pathogenic apicomplexan parasites, Plasmodium falciparum and Toxoplasma gondii . Each of these organisms express two PanK homologues (PanK1 and PanK2). We demonstrate that Pf PanK1 and Pf PanK2 associate, forming a single, functional PanK complex that includes the multi-functional protein, Pf 14-3-3I. Similarly, we demonstrate that Tg PanK1 and Tg PanK2 form a single complex that possesses PanK activity. Both Tg PanK1 and Tg PanK2 are essential for T . gondii proliferation, specifically due to their PanK activity. Our study constitutes the first examples of heteromeric PanK complexes in nature and provides an explanation for the presence of multiple PanKs within certain organisms. Apicomplexans are a phylum of obligate intracellular parasites that cause diseases in humans and other animals, inflicting considerable burdens on human societies. During their intracellular stage, these parasites must scavenge vitamins from their host organisms in order to survive and proliferate. One such vitamin is pantothenate (vitamin B 5 ), which parasites convert in a universal five-step pathway to the essential cofactor coenzyme A (CoA). The first reaction in the CoA biosynthesis pathway is catalysed by the enzyme pantothenate kinase (PanK). The genomes of humans and many other organisms, including apicomplexans, encode multiple PanK homologues, although in all studied examples, the functional PanK enzyme exists as a homodimer. In this study, we demonstrate that the two PanK homologues encoded in the genomes of the apicomplexans Plasmodium falciparum and Toxoplasma gondii , PanK1 and PanK2, exist as functional heteromeric complexes. We provide evidence that both PanK homologues contribute to the PanK activity in these parasites, and that both PanK1 and PanK2 are essential for the proliferation of T . gondii parasites specifically for their PanK activity. Our data describe the first known instances of heteromeric PanK complexes in nature and may explain why some organisms that express multiple PanKs harbour seemingly non-functional isoforms.
Audience Academic
Author van Dooren, Giel G
Howieson, Vanessa M
Saliba, Kevin J
Tjhin, Erick T
Spry, Christina
AuthorAffiliation 1 Research School of Biology, The Australian National University, Canberra, Australia
2 Medical School, The Australian National University, Canberra, Australia
Johns Hopkins Bloomberg School of Public Health, UNITED STATES
AuthorAffiliation_xml – name: 1 Research School of Biology, The Australian National University, Canberra, Australia
– name: Johns Hopkins Bloomberg School of Public Health, UNITED STATES
– name: 2 Medical School, The Australian National University, Canberra, Australia
Author_xml – sequence: 1
  fullname: Tjhin, Erick T
– sequence: 2
  fullname: Howieson, Vanessa M
– sequence: 3
  fullname: Spry, Christina
– sequence: 4
  fullname: van Dooren, Giel G
– sequence: 5
  fullname: Saliba, Kevin J
BookMark eNqVkkuP0zAQxyO0iH3AN0AiEhc4tPgVO74gVSselVYg8Thbk2TSuiR2sN3V8u1xaUAU7QX5YHv8m_88PJfFmfMOi-IpJUvKFX218_vgYFhOE6QlJUQrrR4UF7Sq-EJxJc7-Op8XlzHuCBGUU_moOOeCMyEJvSjWq9L5WxzKLSYMfsRg23ICl3zaooOE5TfrIGLZ-nEa8K60roTJzjdwmQ0QbcL4uHjYwxDxybxfFV_fvvly_X5x8_Hd-np1s2glr9OCcqFQggKqKyoq2tTQ1axqeM26XrNaauh1p1jV91zQhrG6Jh1FrphoGeWcXxXPjrrT4KOZuxANqyTVgigmM7E-Ep2HnZmCHSH8MB6s-WXwYWMgJNsOaBpaqQ5ILxXXAqnWTDVKNB1BLXK6Imu9nqPtmxG7Fl0KMJyInr44uzUbf2tqLqVWJAu8mAWC_77HmMxoY4vDAA79_pB3pXKRSrKMPv8Hvb-6mdpALsC63ue47UHUrKQiuuZUH6jlPVReHY759xz2NttPHF6eOGQm4V3awD5Gs_786T_YD6esOLJt8DEG7P_0jhJzGOTfRZrDIJt5kPlPTYrkxQ
CitedBy_id crossref_primary_10_1016_j_molbiopara_2024_111633
crossref_primary_10_1098_rsob_230463
crossref_primary_10_1038_s41467_022_27996_4
crossref_primary_10_1128_aac_00540_22
crossref_primary_10_3389_fcimb_2023_1175409
crossref_primary_10_1016_j_ijpddr_2023_03_003
crossref_primary_10_1021_acsinfecdis_4c00289
crossref_primary_10_1080_07391102_2023_2260483
Cites_doi 10.1007/s11103-006-0037-4
10.1371/journal.ppat.1006918
10.1042/BST20140158
10.1007/978-1-4939-7737-6_5
10.2174/187152610791163390
10.1371/journal.pone.0011107
10.1038/s41598-017-14074-9
10.1074/jbc.M110.194613
10.1126/stke.2962005re10
10.1021/acsinfecdis.7b00024
10.1128/AAC.49.11.4649-4657.2005
10.1194/jlr.M300279-JLR200
10.1074/jbc.275.2.1377
10.1016/S0898-6568(00)00131-5
10.1016/S0378-1119(02)00564-4
10.1128/JB.00469-06
10.1074/jbc.M506275200
10.1007/978-1-62703-646-7_17
10.1007/s002390010107
10.1093/bioinformatics/17.8.700
10.1016/j.ejmech.2017.08.050
10.1016/S1357-2725(01)00114-5
10.1016/j.molbiopara.2009.09.005
10.1126/scitranslmed.aas9917
10.1074/jbc.273.17.10190
10.1016/j.molbiopara.2006.03.011
10.1021/acs.jmedchem.0c01755
10.1074/jbc.M113.484329
10.1093/hmg/ddi005
10.1099/mic.0.040717-0
10.1002/pmic.200700763
10.1111/j.1574-6976.2007.00093.x
10.1016/j.str.2006.06.008
10.1038/ncomms14455
10.1371/journal.pone.0053179
10.1016/j.molbiopara.2005.02.003
10.1128/AAC.04970-14
10.1002/prot.24841
10.1093/hmg/ddg026
10.1016/j.cell.2016.08.019
10.1107/S0907444906012728
10.1002/pro.3611
10.1038/ng572
10.1016/j.chembiol.2007.01.013
10.1002/prot.24290
10.1021/acsinfecdis.0c00075
10.1074/jbc.M116.748061
10.1021/bi062299p
10.1016/j.febslet.2013.07.052
10.1186/s12936-017-1839-3
10.1002/cmdc.201800327
10.1074/jbc.M003190200
10.1016/j.ab.2013.12.010
10.1002/bies.1134
10.1128/AAC.49.2.632-637.2005
10.1016/B978-0-12-396481-6.00017-9
10.1128/AAC.01436-16
10.1074/jbc.M306321200
10.3389/fcimb.2021.639065
10.1186/s13071-016-1860-3
10.1016/S0166-6851(99)00090-0
10.1074/jbc.C500044200
10.1016/j.chembiol.2010.06.006
10.1371/journal.pone.0054974
10.1128/mBio.01114-14
10.1186/1475-2875-13-343
10.1016/j.plipres.2005.04.001
10.1186/s12936-015-0673-8
10.1073/pnas.0803862105
10.1016/S0021-9258(18)43098-0
10.1016/0962-8924(96)10029-5
10.1074/jbc.M010942200
ContentType Journal Article
Copyright COPYRIGHT 2021 Public Library of Science
2021 Tjhin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2021 Tjhin et al 2021 Tjhin et al
Copyright_xml – notice: COPYRIGHT 2021 Public Library of Science
– notice: 2021 Tjhin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2021 Tjhin et al 2021 Tjhin et al
DBID AAYXX
CITATION
ISN
ISR
3V.
7QL
7U9
7X7
7XB
88E
8FE
8FH
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
C1K
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
H94
HCIFZ
K9.
LK8
M0S
M1P
M7P
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
DOA
DOI 10.1371/journal.ppat.1009797
DatabaseName CrossRef
Gale In Context: Canada
Science in Context
ProQuest Central (Corporate)
Bacteriology Abstracts (Microbiology B)
Virology and AIDS Abstracts
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
ProQuest SciTech Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central
ProQuest Central Essentials
Biological Science Collection
AUTh Library subscriptions: ProQuest Central
Natural Science Collection
Environmental Sciences and Pollution Management
ProQuest One Community College
ProQuest Central Korea
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
AIDS and Cancer Research Abstracts
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
Biological Sciences
Health & Medical Collection (Alumni Edition)
PML(ProQuest Medical Library)
Biological Science Database
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
PubMed Central (Full Participant titles)
Directory of Open Access Journals
DatabaseTitle CrossRef
Publicly Available Content Database
ProQuest Central Student
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central China
Environmental Sciences and Pollution Management
ProQuest Central
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Bacteriology Abstracts (Microbiology B)
Biological Science Collection
AIDS and Cancer Research Abstracts
ProQuest Medical Library (Alumni)
Virology and AIDS Abstracts
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList Publicly Available Content Database

CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
DocumentTitleAlternate The pantothenate kinase of apicomplexan parasites
EISSN 1553-7374
Editor Prigge, Sean T.
Editor_xml – sequence: 1
  givenname: Sean T.
  surname: Prigge
  fullname: Prigge, Sean T.
EndPage e1009797
ExternalDocumentID 2561940726
oai_doaj_org_article_b157da0f67394e19927b74bd0e946384
A670983196
10_1371_journal_ppat_1009797
GeographicLocations Australia
GeographicLocations_xml – name: Australia
GrantInformation_xml – fundername: ;
– fundername: ;
  grantid: APP1129843
– fundername: ;
  grantid: 1016357
– fundername: ;
  grantid: DP150102883
GroupedDBID ---
123
29O
2WC
3V.
53G
5VS
7X7
88E
8FE
8FH
8FI
8FJ
AAFWJ
AAYXX
ABDBF
ABUWG
ACGFO
ACIHN
ACPRK
ADBBV
AEAQA
AENEX
AFKRA
AFPKN
AFRAH
AHMBA
ALMA_UNASSIGNED_HOLDINGS
AOIJS
B0M
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BVXVI
BWKFM
CCPQU
CITATION
CS3
DIK
DU5
E3Z
EAP
EAS
EBD
EMK
EMOBN
ESX
F5P
FPL
FYUFA
GROUPED_DOAJ
GX1
HCIFZ
HMCUK
HYE
IAO
IHR
INH
INR
ISN
ISR
ITC
KQ8
LK8
M1P
M48
M7P
MM.
M~E
O5R
O5S
OK1
P2P
PGMZT
PIMPY
PQQKQ
PROAC
PSQYO
PV9
QF4
QN7
RNS
RPM
RZL
SV3
TR2
TUS
UKHRP
WOW
~8M
7QL
7U9
7XB
8FK
AZQEC
C1K
DWQXO
GNUQQ
H94
K9.
PQEST
PQUKI
PRINS
7X8
5PM
AAPBV
ABPTK
ID FETCH-LOGICAL-c638t-1347e6a7a1951451b8ad825b382df92869af9d725ff341b22880d1e3724c21333
IEDL.DBID RPM
ISSN 1553-7374
1553-7366
IngestDate Sun Nov 05 00:10:31 EDT 2023
Tue Oct 22 15:15:59 EDT 2024
Tue Sep 17 21:26:16 EDT 2024
Fri Aug 16 05:14:34 EDT 2024
Thu Oct 10 16:17:12 EDT 2024
Tue Nov 19 21:13:10 EST 2024
Tue Nov 12 22:39:43 EST 2024
Thu Aug 01 19:18:05 EDT 2024
Thu Aug 01 19:55:39 EDT 2024
Thu Nov 21 21:21:05 EST 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 7
Language English
License This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Creative Commons Attribution License
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c638t-1347e6a7a1951451b8ad825b382df92869af9d725ff341b22880d1e3724c21333
Notes new_version
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
The authors have declared that no competing interests exist.
ORCID 0000-0003-1711-0815
0000-0001-9052-2037
0000-0003-3345-8440
0000-0003-2455-9821
0000-0002-8156-7070
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366970/
PMID 34324601
PQID 2561940726
PQPubID 1436335
ParticipantIDs plos_journals_2561940726
doaj_primary_oai_doaj_org_article_b157da0f67394e19927b74bd0e946384
pubmedcentral_primary_oai_pubmedcentral_nih_gov_8366970
proquest_miscellaneous_2557228762
proquest_journals_2561940726
gale_infotracmisc_A670983196
gale_infotracacademiconefile_A670983196
gale_incontextgauss_ISR_A670983196
gale_incontextgauss_ISN_A670983196
crossref_primary_10_1371_journal_ppat_1009797
PublicationCentury 2000
PublicationDate 20210729
PublicationDateYYYYMMDD 2021-07-29
PublicationDate_xml – month: 7
  year: 2021
  text: 20210729
  day: 29
PublicationDecade 2020
PublicationPlace San Francisco
PublicationPlace_xml – name: San Francisco
– name: San Francisco, CA USA
PublicationTitle PLoS pathogens
PublicationYear 2021
Publisher Public Library of Science
Public Library of Science (PLoS)
Publisher_xml – name: Public Library of Science
– name: Public Library of Science (PLoS)
References C Spry (ppat.1009797.ref064) 2018; 143
ET Tjhin (ppat.1009797.ref071) 2013; 587
J Guan (ppat.1009797.ref067) 2021; 64
S Das (ppat.1009797.ref004) 2006; 62
S Warrenfeltz (ppat.1009797.ref068) 2018; 1757
KJ Saliba (ppat.1009797.ref052) 2005; 49
R Leonardi (ppat.1009797.ref034) 2010; 17
KJ Saliba (ppat.1009797.ref042) 2001; 276
GG van Dooren (ppat.1009797.ref073) 2008; 105
B Li (ppat.1009797.ref009) 2013; 81
K Hörtnagel (ppat.1009797.ref019) 2003; 12
BS Hong (ppat.1009797.ref006) 2006; 14
D Shen (ppat.1009797.ref038) 2008; 48
J Guan (ppat.1009797.ref065) 2018; 13
M Somogyi (ppat.1009797.ref075) 1945; 160
PG Crichton (ppat.1009797.ref044) 2013; 288
R Leonardi (ppat.1009797.ref001) 2005; 44
J Yao (ppat.1009797.ref026) 2019; 28
M de Villiers (ppat.1009797.ref062) 2017; 3
SM Sidik (ppat.1009797.ref029) 2016; 166
E Rajendran (ppat.1009797.ref036) 2017; 8
C Mehlin (ppat.1009797.ref037) 2006; 148
F Di Girolamo (ppat.1009797.ref046) 2008; 8
K Yang (ppat.1009797.ref005) 2006; 188
A Aitken (ppat.1009797.ref031) 1996; 6
CJ Macuamule (ppat.1009797.ref058) 2015; 59
LA Brand (ppat.1009797.ref025) 2005; 280
J Schalkwijk (ppat.1009797.ref040) 2019; 11
HE Pett (ppat.1009797.ref059) 2015; 14
MC Franklin (ppat.1009797.ref010) 2015; 83
M Yun (ppat.1009797.ref003) 2000; 275
D Awasthy (ppat.1009797.ref023) 2010; 156
MJ van Hemert (ppat.1009797.ref049) 2001; 23
J Pei (ppat.1009797.ref077) 2001; 17
AJ Muslin (ppat.1009797.ref050) 2000; 12
M Zhang (ppat.1009797.ref030) 2018
KJ Saliba (ppat.1009797.ref053) 2005; 141
T Weidner (ppat.1009797.ref063) 2017; 16
Y-M Zhang (ppat.1009797.ref016) 2005; 280
C Subramanian (ppat.1009797.ref035) 2016; 291
KJ Saliba (ppat.1009797.ref057) 2014; 42
T Kupke (ppat.1009797.ref011) 2003; 278
RJW Allen (ppat.1009797.ref069) 2010; 169
X Ni (ppat.1009797.ref018) 2002; 34
M Rosenquist (ppat.1009797.ref048) 2000; 51
C Spry (ppat.1009797.ref054) 2005; 49
G Ramaswamy (ppat.1009797.ref020) 2004; 45
D Bridges (ppat.1009797.ref051) 2005; 2005
D Jacot (ppat.1009797.ref070) 2014
VM Howieson (ppat.1009797.ref060) 2016; 60
C Spry (ppat.1009797.ref002) 2008; 32
RR Yocum (ppat.1009797.ref024) 2004
KJ Saliba (ppat.1009797.ref074) 1998; 273
J Pei (ppat.1009797.ref076) 2014; 1079
EG Dastidar (ppat.1009797.ref047) 2013; 8
KM Brown (ppat.1009797.ref033) 2017
GB Tilton (ppat.1009797.ref012) 2006; 61
S Fletcher (ppat.1009797.ref061) 2016; 9
A Nurkanto (ppat.1009797.ref041) 2021; 11
C Spry (ppat.1009797.ref066) 2020; 6
Y-M Zhang (ppat.1009797.ref021) 2007; 14
C Spry (ppat.1009797.ref028) 2010; 10
BS Hong (ppat.1009797.ref008) 2007; 282
NI Nicely (ppat.1009797.ref007) 2007; 46
B Zhou (ppat.1009797.ref017) 2001; 28
CO Rock (ppat.1009797.ref013) 2000; 275
M Lalle (ppat.1009797.ref032) 2011; 286
JE Chiu (ppat.1009797.ref039) 2017; 7
C Spry (ppat.1009797.ref043) 2014; 451
B Al-Khedery (ppat.1009797.ref045) 1999; 102
R Leonardi (ppat.1009797.ref022) 2010; 5
C Spry (ppat.1009797.ref055) 2013; 8
B Shen (ppat.1009797.ref072) 2014; 5
S Fletcher (ppat.1009797.ref056) 2014; 13
CO Rock (ppat.1009797.ref014) 2002; 291
YM Kuo (ppat.1009797.ref015) 2005; 14
ET Tjhin (ppat.1009797.ref027) 2018; 14
References_xml – volume: 61
  start-page: 629
  year: 2006
  ident: ppat.1009797.ref012
  article-title: Plant coenzyme A biosynthesis: characterization of two pantothenate kinases from Arabidopsis
  publication-title: Plant Mol Biol
  doi: 10.1007/s11103-006-0037-4
  contributor:
    fullname: GB Tilton
– volume: 14
  start-page: e1006918
  year: 2018
  ident: ppat.1009797.ref027
  article-title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
  publication-title: PLoS Pathog
  doi: 10.1371/journal.ppat.1006918
  contributor:
    fullname: ET Tjhin
– volume: 42
  start-page: 1087
  year: 2014
  ident: ppat.1009797.ref057
  article-title: Exploiting the coenzyme A biosynthesis pathway for the identification of new antimalarial agents: the case for pantothenamides
  publication-title: Biochem Soc Trans
  doi: 10.1042/BST20140158
  contributor:
    fullname: KJ Saliba
– volume: 1757
  start-page: 69
  year: 2018
  ident: ppat.1009797.ref068
  article-title: EuPathDB: the Eukaryotic Pathogen Genomics Database Resource
  publication-title: Methods Mol Biol
  doi: 10.1007/978-1-4939-7737-6_5
  contributor:
    fullname: S Warrenfeltz
– volume: 10
  start-page: 200
  year: 2010
  ident: ppat.1009797.ref028
  article-title: Pantothenate utilization by Plasmodium as a target for antimalarial chemotherapy
  publication-title: Infect Disord Drug Targets
  doi: 10.2174/187152610791163390
  contributor:
    fullname: C Spry
– volume: 5
  start-page: e11107
  year: 2010
  ident: ppat.1009797.ref022
  article-title: Pantothenate kinase 1 is required to support the metabolic transition from the fed to the fasted state
  publication-title: PLoS One.
  doi: 10.1371/journal.pone.0011107
  contributor:
    fullname: R Leonardi
– volume: 7
  start-page: 14234
  year: 2017
  ident: ppat.1009797.ref039
  article-title: The antimalarial activity of the pantothenamide α-PanAm is via inhibition of pantothenate phosphorylation
  publication-title: Sci Rep
  doi: 10.1038/s41598-017-14074-9
  contributor:
    fullname: JE Chiu
– volume: 286
  start-page: 1227
  year: 2011
  ident: ppat.1009797.ref032
  article-title: Dematin, a component of the erythrocyte membrane skeleton, is internalized by the malaria parasite and associates with Plasmodium 14-3-3
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M110.194613
  contributor:
    fullname: M Lalle
– volume: 2005
  start-page: re10
  year: 2005
  ident: ppat.1009797.ref051
  article-title: 14-3-3 proteins: a number of functions for a numbered protein
  publication-title: Sci STKE
  doi: 10.1126/stke.2962005re10
  contributor:
    fullname: D Bridges
– volume: 3
  start-page: 527
  year: 2017
  ident: ppat.1009797.ref062
  article-title: Antiplasmodial mode of action of pantothenamides: pantothenate kinase serves as a metabolic activator not as a target
  publication-title: ACS Infect Dis.
  doi: 10.1021/acsinfecdis.7b00024
  contributor:
    fullname: M de Villiers
– volume: 49
  start-page: 4649
  year: 2005
  ident: ppat.1009797.ref054
  article-title: A class of pantothenic acid analogs inhibits Plasmodium falciparum pantothenate kinase and represses the proliferation of malaria parasites
  publication-title: Antimicrob Agents Chemother
  doi: 10.1128/AAC.49.11.4649-4657.2005
  contributor:
    fullname: C Spry
– volume: 45
  start-page: 17
  year: 2004
  ident: ppat.1009797.ref020
  article-title: PPARα controls the intracellular coenzyme A concentration via regulation of PANK1α gene expression
  publication-title: J Lipid Res
  doi: 10.1194/jlr.M300279-JLR200
  contributor:
    fullname: G Ramaswamy
– volume: 275
  start-page: 1377
  year: 2000
  ident: ppat.1009797.ref013
  article-title: Pantothenate kinase regulation of the intracellular concentration of coenzyme A
  publication-title: J Biol Chem
  doi: 10.1074/jbc.275.2.1377
  contributor:
    fullname: CO Rock
– volume: 12
  start-page: 703
  year: 2000
  ident: ppat.1009797.ref050
  article-title: 14-3-3 proteins: regulation of subcellular localization by molecular interference
  publication-title: Cell Signal
  doi: 10.1016/S0898-6568(00)00131-5
  contributor:
    fullname: AJ Muslin
– start-page: 8
  year: 2017
  ident: ppat.1009797.ref033
  article-title: Plasma membrane association by N-acylation governs PKG function in Toxoplasma gondii
  publication-title: MBio
  contributor:
    fullname: KM Brown
– volume: 291
  start-page: 35
  year: 2002
  ident: ppat.1009797.ref014
  article-title: The murine pantothenate kinase (Pank1) gene encodes two differentially regulated pantothenate kinase isozymes
  publication-title: Gene
  doi: 10.1016/S0378-1119(02)00564-4
  contributor:
    fullname: CO Rock
– volume: 188
  start-page: 5532
  year: 2006
  ident: ppat.1009797.ref005
  article-title: Crystal structure of a type III pantothenate kinase: insight into the mechanism of an essential coenzyme A biosynthetic enzyme universally distributed in bacteria
  publication-title: J Bacteriol
  doi: 10.1128/JB.00469-06
  contributor:
    fullname: K Yang
– volume: 280
  start-page: 32594
  year: 2005
  ident: ppat.1009797.ref016
  article-title: Feedback regulation of murine pantothenate kinase 3 by coenzyme A and coenzyme A thioesters
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M506275200
  contributor:
    fullname: Y-M Zhang
– volume: 1079
  start-page: 263
  year: 2014
  ident: ppat.1009797.ref076
  article-title: PROMALS3D: multiple protein sequence alignment enhanced with evolutionary and three-dimensional structural information
  publication-title: Methods Mol Biol
  doi: 10.1007/978-1-62703-646-7_17
  contributor:
    fullname: J Pei
– volume: 51
  start-page: 446
  year: 2000
  ident: ppat.1009797.ref048
  article-title: Evolution of the 14-3-3 protein family: does the large number of isoforms in multicellular organisms reflect functional specificity?
  publication-title: J Mol Evol
  doi: 10.1007/s002390010107
  contributor:
    fullname: M Rosenquist
– volume: 17
  start-page: 700
  year: 2001
  ident: ppat.1009797.ref077
  article-title: AL2CO: calculation of positional conservation in a protein sequence alignment
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/17.8.700
  contributor:
    fullname: J Pei
– volume: 48
  start-page: 403
  year: 2008
  ident: ppat.1009797.ref038
  article-title: Cloning and confirmation of several potential pantothenate kinases and their interaction with pantothenate analogues
  publication-title: J Tsinghua Univ
  contributor:
    fullname: D Shen
– volume: 143
  start-page: 1139
  year: 2018
  ident: ppat.1009797.ref064
  article-title: Structure-activity analysis of CJ-15,801 analogues that interact with Plasmodium falciparum pantothenate kinase and inhibit parasite proliferation
  publication-title: Eur J Med Chem
  doi: 10.1016/j.ejmech.2017.08.050
  contributor:
    fullname: C Spry
– volume: 34
  start-page: 109
  year: 2002
  ident: ppat.1009797.ref018
  article-title: Cloning and characterization of a novel human pantothenate kinase gene
  publication-title: Int J Biochem Cell Biol
  doi: 10.1016/S1357-2725(01)00114-5
  contributor:
    fullname: X Ni
– volume: 169
  start-page: 63
  year: 2010
  ident: ppat.1009797.ref069
  article-title: Plasmodium falciparum culture: the benefits of shaking
  publication-title: Mol Biochem Parasitol
  doi: 10.1016/j.molbiopara.2009.09.005
  contributor:
    fullname: RJW Allen
– volume: 11
  start-page: eaas9917
  year: 2019
  ident: ppat.1009797.ref040
  article-title: Antimalarial pantothenamide metabolites target acetyl-coenzyme A biosynthesis in Plasmodium falciparum
  publication-title: Sci Transl Med
  doi: 10.1126/scitranslmed.aas9917
  contributor:
    fullname: J Schalkwijk
– volume: 273
  start-page: 10190
  year: 1998
  ident: ppat.1009797.ref074
  article-title: Transport and metabolism of the essential vitamin pantothenic acid in human erythrocytes infected with the malaria parasite Plasmodium falciparum
  publication-title: J Biol Chem
  doi: 10.1074/jbc.273.17.10190
  contributor:
    fullname: KJ Saliba
– volume: 148
  start-page: 144
  year: 2006
  ident: ppat.1009797.ref037
  article-title: Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes
  publication-title: Mol Biochem Parasitol
  doi: 10.1016/j.molbiopara.2006.03.011
  contributor:
    fullname: C Mehlin
– volume: 64
  start-page: 4478
  year: 2021
  ident: ppat.1009797.ref067
  article-title: Exploring heteroaromatic rings as a replacement for the labile amide of antiplasmodial pantothenamides
  publication-title: J Med Chem
  doi: 10.1021/acs.jmedchem.0c01755
  contributor:
    fullname: J Guan
– volume: 288
  start-page: 22163
  year: 2013
  ident: ppat.1009797.ref044
  article-title: Lipid, detergent, and Coomassie Blue G-250 affect the migration of small membrane proteins in blue native gels: mitochondrial carriers migrate as monomers not dimers
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M113.484329
  contributor:
    fullname: PG Crichton
– volume: 14
  start-page: 49
  year: 2005
  ident: ppat.1009797.ref015
  article-title: Deficiency of pantothenate kinase 2 (Pank2) in mice leads to retinal degeneration and azoospermia
  publication-title: Hum Mol Genet
  doi: 10.1093/hmg/ddi005
  contributor:
    fullname: YM Kuo
– volume: 156
  start-page: 2691
  year: 2010
  ident: ppat.1009797.ref023
  article-title: Essentiality and functional analysis of type I and type III pantothenate kinases of Mycobacterium tuberculosis
  publication-title: Microbiology
  doi: 10.1099/mic.0.040717-0
  contributor:
    fullname: D Awasthy
– volume: 8
  start-page: 2500
  year: 2008
  ident: ppat.1009797.ref046
  article-title: Plasmodium lipid rafts contain proteins implicated in vesicular trafficking and signalling as well as members of the PIR superfamily, potentially implicated in host immune system interactions
  publication-title: Proteomics
  doi: 10.1002/pmic.200700763
  contributor:
    fullname: F Di Girolamo
– volume: 32
  start-page: 56
  year: 2008
  ident: ppat.1009797.ref002
  article-title: Coenzyme A biosynthesis: an antimicrobial drug target
  publication-title: FEMS Microbiol Rev
  doi: 10.1111/j.1574-6976.2007.00093.x
  contributor:
    fullname: C Spry
– volume: 14
  start-page: 1251
  year: 2006
  ident: ppat.1009797.ref006
  article-title: Prokaryotic type II and type III pantothenate kinases: The same monomer fold creates dimers with distinct catalytic properties
  publication-title: Structure
  doi: 10.1016/j.str.2006.06.008
  contributor:
    fullname: BS Hong
– volume: 8
  start-page: 14455
  year: 2017
  ident: ppat.1009797.ref036
  article-title: Cationic amino acid transporters play key roles in the survival and transmission of apicomplexan parasites
  publication-title: Nat Commun
  doi: 10.1038/ncomms14455
  contributor:
    fullname: E Rajendran
– volume: 8
  start-page: e53179
  year: 2013
  ident: ppat.1009797.ref047
  article-title: Comprehensive histone phosphorylation analysis and identification of Pf14-3-3 protein as a histone H3 phosphorylation reader in malaria parasites
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0053179
  contributor:
    fullname: EG Dastidar
– volume: 141
  start-page: 129
  year: 2005
  ident: ppat.1009797.ref053
  article-title: CJ-15,801, a fungal natural product, inhibits the intraerythrocytic stage of Plasmodium falciparum in vitro via an effect on pantothenic acid utilisation
  publication-title: Mol Biochem Parasitol
  doi: 10.1016/j.molbiopara.2005.02.003
  contributor:
    fullname: KJ Saliba
– volume: 59
  start-page: 3666
  year: 2015
  ident: ppat.1009797.ref058
  article-title: A pantetheinase-resistant pantothenamide with potent, on-target, and selective antiplasmodial activity
  publication-title: Antimicrob Agents Chemother
  doi: 10.1128/AAC.04970-14
  contributor:
    fullname: CJ Macuamule
– volume: 83
  start-page: 2124
  year: 2015
  ident: ppat.1009797.ref010
  article-title: Structural genomics for drug design against the pathogen Coxiella burnetii
  publication-title: Proteins
  doi: 10.1002/prot.24841
  contributor:
    fullname: MC Franklin
– volume: 12
  start-page: 321
  year: 2003
  ident: ppat.1009797.ref019
  article-title: An isoform of hPANK2, deficient in pantothenate kinase-associated neurodegeneration, localizes to mitochondria
  publication-title: Hum Mol Genet
  doi: 10.1093/hmg/ddg026
  contributor:
    fullname: K Hörtnagel
– volume: 166
  start-page: 1423
  year: 2016
  ident: ppat.1009797.ref029
  article-title: A genome-wide CRISPR screen in Toxoplasma identifies essential apicomplexan genes
  publication-title: Cell
  doi: 10.1016/j.cell.2016.08.019
  contributor:
    fullname: SM Sidik
– volume: 62
  start-page: 628
  year: 2006
  ident: ppat.1009797.ref004
  article-title: Invariance and variability in bacterial PanK: a study based on the crystal structure of Mycobacterium tuberculosis PanK
  publication-title: Acta Crystallogr D Biol Crystallogr
  doi: 10.1107/S0907444906012728
  contributor:
    fullname: S Das
– volume: 28
  start-page: 1031
  year: 2019
  ident: ppat.1009797.ref026
  article-title: Human pantothenate kinase 4 is a pseudo-pantothenate kinase
  publication-title: Protein Sci
  doi: 10.1002/pro.3611
  contributor:
    fullname: J Yao
– volume: 28
  start-page: 345
  year: 2001
  ident: ppat.1009797.ref017
  article-title: A novel pantothenate kinase gene (PANK2) is defective in Hallervorden-Spatz syndrome
  publication-title: Nat Genet
  doi: 10.1038/ng572
  contributor:
    fullname: B Zhou
– volume: 14
  start-page: 291
  year: 2007
  ident: ppat.1009797.ref021
  article-title: Chemical knockout of pantothenate kinase reveals the metabolic and genetic program responsible for hepatic coenzyme A homeostasis
  publication-title: Chem Biol
  doi: 10.1016/j.chembiol.2007.01.013
  contributor:
    fullname: Y-M Zhang
– volume: 282
  start-page: 27984
  year: 2007
  ident: ppat.1009797.ref008
  article-title: Crystal structures of human pantothenate kinases. Insights into allosteric regulation and mutations linked to a neurodegeneration disorder
  publication-title: J Biol Chem
  contributor:
    fullname: BS Hong
– volume: 81
  start-page: 1466
  year: 2013
  ident: ppat.1009797.ref009
  article-title: Crystal structures of Klebsiella pneumoniae pantothenate kinase in complex with N-substituted pantothenamides
  publication-title: Proteins
  doi: 10.1002/prot.24290
  contributor:
    fullname: B Li
– volume: 6
  start-page: 1844
  year: 2020
  ident: ppat.1009797.ref066
  article-title: Toward a stable and potent coenzyme A-targeting antiplasmodial agent: structure-activity relationship studies of N-phenethyl-α-methyl-pantothenamide.
  publication-title: ACS Infect Dis.
  doi: 10.1021/acsinfecdis.0c00075
  contributor:
    fullname: C Spry
– volume: 291
  start-page: 22302
  year: 2016
  ident: ppat.1009797.ref035
  article-title: Allosteric regulation of mammalian pantothenate kinase
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M116.748061
  contributor:
    fullname: C Subramanian
– volume: 46
  start-page: 3234
  year: 2007
  ident: ppat.1009797.ref007
  article-title: Structure of the type III pantothenate kinase from Bacillus anthracis at 2.0 Å resolution: implications for coenzyme A-dependent redox biology
  publication-title: Biochemistry
  doi: 10.1021/bi062299p
  contributor:
    fullname: NI Nicely
– volume: 587
  start-page: 3182
  year: 2013
  ident: ppat.1009797.ref071
  article-title: Studies with the Plasmodium falciparum hexokinase reveal that PfHT limits the rate of glucose entry into glycolysis
  publication-title: FEBS Lett
  doi: 10.1016/j.febslet.2013.07.052
  contributor:
    fullname: ET Tjhin
– volume: 16
  start-page: 192
  year: 2017
  ident: ppat.1009797.ref063
  article-title: Antiplasmodial dihetarylthioethers target the coenzyme A synthesis pathway in Plasmodium falciparum erythrocytic stages
  publication-title: Malar J
  doi: 10.1186/s12936-017-1839-3
  contributor:
    fullname: T Weidner
– volume: 13
  start-page: 2677
  year: 2018
  ident: ppat.1009797.ref065
  article-title: Structure-activity relationships of antiplasmodial pantothenamide analogues reveal a new way by which triazoles mimic amide bonds
  publication-title: ChemMedChem
  doi: 10.1002/cmdc.201800327
  contributor:
    fullname: J Guan
– volume: 275
  start-page: 28093
  year: 2000
  ident: ppat.1009797.ref003
  article-title: Structural basis for the feedback regulation of Escherichia coli pantothenate kinase by coenzyme A
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M003190200
  contributor:
    fullname: M Yun
– year: 2004
  ident: ppat.1009797.ref024
  publication-title: Microorganisms and assays for the identification of antibiotics
  contributor:
    fullname: RR Yocum
– volume: 451
  start-page: 76
  year: 2014
  ident: ppat.1009797.ref043
  article-title: A miniaturized assay for measuring small molecule phosphorylation in the presence of complex matrices
  publication-title: Anal Biochem
  doi: 10.1016/j.ab.2013.12.010
  contributor:
    fullname: C Spry
– volume: 23
  start-page: 936
  year: 2001
  ident: ppat.1009797.ref049
  article-title: 14-3-3 proteins: key regulators of cell division, signalling and apoptosis
  publication-title: Bioessays
  doi: 10.1002/bies.1134
  contributor:
    fullname: MJ van Hemert
– volume: 49
  start-page: 632
  year: 2005
  ident: ppat.1009797.ref052
  article-title: Provitamin B5 (pantothenol) inhibits growth of the intraerythrocytic malaria parasite
  publication-title: Antimicrob Agents Chemother
  doi: 10.1128/AAC.49.2.632-637.2005
  contributor:
    fullname: KJ Saliba
– start-page: 577
  year: 2014
  ident: ppat.1009797.ref070
  article-title: Genetic manipulation of Toxoplasma gondii.
  publication-title: Toxoplasma gondii. Elsevier
  doi: 10.1016/B978-0-12-396481-6.00017-9
  contributor:
    fullname: D Jacot
– volume: 60
  start-page: 7146
  year: 2016
  ident: ppat.1009797.ref060
  article-title: Triazole substitution of a labile amide bond stabilizes pantothenamides and improves their antiplasmodial potency
  publication-title: Antimicrob Agents Chemother
  doi: 10.1128/AAC.01436-16
  contributor:
    fullname: VM Howieson
– volume: 278
  start-page: 38229
  year: 2003
  ident: ppat.1009797.ref011
  article-title: 4’-Phosphopantetheine and coenzyme A biosynthesis in plants
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M306321200
  contributor:
    fullname: T Kupke
– volume: 11
  start-page: 639065
  year: 2021
  ident: ppat.1009797.ref041
  article-title: Characterization of Plasmodium falciparum pantothenate kinase and identification of its inhibitors from natural products
  publication-title: Front Cell Infect Microbiol
  doi: 10.3389/fcimb.2021.639065
  contributor:
    fullname: A Nurkanto
– volume: 9
  start-page: 589
  year: 2016
  ident: ppat.1009797.ref061
  article-title: Biological characterization of chemically diverse compounds targeting the Plasmodium falciparum coenzyme A synthesis pathway
  publication-title: Parasit Vectors
  doi: 10.1186/s13071-016-1860-3
  contributor:
    fullname: S Fletcher
– volume: 102
  start-page: 117
  year: 1999
  ident: ppat.1009797.ref045
  article-title: Stage-specific expression of 14-3-3 in asexual blood-stage Plasmodium
  publication-title: Mol Biochem Parasitol
  doi: 10.1016/S0166-6851(99)00090-0
  contributor:
    fullname: B Al-Khedery
– volume: 280
  start-page: 20185
  year: 2005
  ident: ppat.1009797.ref025
  article-title: Characterization of a new pantothenate kinase isoform from Helicobacter pylori
  publication-title: J Biol Chem
  doi: 10.1074/jbc.C500044200
  contributor:
    fullname: LA Brand
– volume: 17
  start-page: 892
  year: 2010
  ident: ppat.1009797.ref034
  article-title: Modulation of pantothenate kinase 3 activity by small molecules that interact with the substrate/allosteric regulatory domain
  publication-title: Chem Biol
  doi: 10.1016/j.chembiol.2010.06.006
  contributor:
    fullname: R Leonardi
– volume: 8
  start-page: e54974
  year: 2013
  ident: ppat.1009797.ref055
  article-title: Pantothenamides are potent, on-target inhibitors of Plasmodium falciparum growth when serum pantetheinase is inactivated
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0054974
  contributor:
    fullname: C Spry
– volume: 5
  start-page: e01114
  year: 2014
  ident: ppat.1009797.ref072
  article-title: Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR/CAS9
  publication-title: MBio
  doi: 10.1128/mBio.01114-14
  contributor:
    fullname: B Shen
– volume: 13
  start-page: 343
  year: 2014
  ident: ppat.1009797.ref056
  article-title: A novel approach for the discovery of chemically diverse anti-malarial compounds targeting the Plasmodium falciparum Coenzyme A synthesis pathway.
  publication-title: Malar J
  doi: 10.1186/1475-2875-13-343
  contributor:
    fullname: S Fletcher
– volume: 44
  start-page: 125
  year: 2005
  ident: ppat.1009797.ref001
  article-title: Coenzyme A: back in action
  publication-title: Prog Lipid Res
  doi: 10.1016/j.plipres.2005.04.001
  contributor:
    fullname: R Leonardi
– volume: 14
  start-page: 169
  year: 2015
  ident: ppat.1009797.ref059
  article-title: Novel pantothenate derivatives for anti-malarial chemotherapy.
  publication-title: Malar J
  doi: 10.1186/s12936-015-0673-8
  contributor:
    fullname: HE Pett
– start-page: 360
  year: 2018
  ident: ppat.1009797.ref030
  article-title: Uncovering the essential genes of the human malaria parasite Plasmodium falciparum by saturation mutagenesis
  publication-title: Science
  contributor:
    fullname: M Zhang
– volume: 105
  start-page: 13574
  year: 2008
  ident: ppat.1009797.ref073
  article-title: Toxoplasma gondii Tic20 is essential for apicoplast protein import
  publication-title: Proc Natl Acad Sci USA
  doi: 10.1073/pnas.0803862105
  contributor:
    fullname: GG van Dooren
– volume: 160
  start-page: 69
  year: 1945
  ident: ppat.1009797.ref075
  article-title: Determination of blood sugar
  publication-title: J Biol Chem
  doi: 10.1016/S0021-9258(18)43098-0
  contributor:
    fullname: M Somogyi
– volume: 6
  start-page: 341
  year: 1996
  ident: ppat.1009797.ref031
  article-title: 14-3-3 and its possible role in co-ordinating multiple signalling pathways
  publication-title: Trends Cell Biol
  doi: 10.1016/0962-8924(96)10029-5
  contributor:
    fullname: A Aitken
– volume: 276
  start-page: 18115
  year: 2001
  ident: ppat.1009797.ref042
  article-title: H+-coupled pantothenate transport in the intracellular malaria parasite
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M010942200
  contributor:
    fullname: KJ Saliba
SSID ssj0041316
Score 2.4564788
Snippet Coenzyme A is synthesised from pantothenate via five enzyme-mediated steps. The first step is catalysed by pantothenate kinase (PanK). All PanKs characterised...
SourceID plos
doaj
pubmedcentral
proquest
gale
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
StartPage e1009797
SubjectTerms Analysis
Biology and Life Sciences
Chemical properties
Coenzyme A
Coenzymes
Enzyme kinetics
Experiments
Genomes
Homology
Kinases
Medicine and Health Sciences
Mutation
Organisms
Pantothenate kinase
Parasites
Peptides
Phosphorylation
Plasmodium falciparum
Proteins
Research and Analysis Methods
Toxoplasma
SummonAdditionalLinks – databaseName: Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3fi9QwEA6yIPgi_uRWT4ki-FSvTdJO87iKx53gPagH9xaSNPUWj7TYW7n7751Ju8sWFF98bDMtzTeTzKSZfMPYm7Js8jbUPiu0C5nSUmVW2gIvc-98sEIm2sXPZ9XJufp0UV7slfqinLCRHngE7sgVJTQ2byuQWgVKlgQHyjV50AptZ2QCzcV2MTXOwTgzp6KnVBQnA1lV06E5CcXRpKN3fW-JPjrXQIRPe04pcffvZuhFf9UNs_Bznjy5542OH7D7UxjJV-PnP2R3QnzE7o6FJW8fs9MVj92vcMUvKdulS7syvKd6wUR2gOEl_7GO6L94yigPN3wdue3X05WNnBjBaV95eMLOjz9--3CSTUUTMo9wUGl5BaGyYAuMnVRZuNo2uAp0shZNq0VdadvqBkTZtujAnBA4gJsiSBDKC1ywyqdsEbsYDhivvQKH8ZklljwotWt9Q38MA1jvRC6WLNuiZvqRG8OkDTLANcWIgyGUzYTykr0naHeyxGydbqC-zaRv8y99L9lrUowh7opIyTHf7WYYzOnXM7MiLrqa5pS_Cn2ZCb2dhNoONentdCABO0-cWDPJw5kkjkA_az4gI9n2eTAYRhaamOfoya3h_Ln51a6ZXkoJbzF0G5IpAXWDrmrJYGZwM_jmLXF9mQjCa7R4Dfmz_4H3c3ZPUBpPDpnQh2xx_XMTXmAcdu1epiH3GwIeLV0
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: Health & Medical Collection
  dbid: 7X7
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3db9MwELegCIkXxKdWGMggJJ7CEsfJxU-oIKYNiT0Ak_pm2Y6zVUxOWFYE_z13rlsWCXhMfW3l-z778jvGXlVVm3e-cVmhrM-kKmVmSlPgY-6s80aUEXbx00l9dCo_LqtlOnAbU1vl1idGR932js7IDzA0Y72dg6jfDt8zmhpFt6tphMZNdqsQGMpRn2G5K7jQP8fRpzQaJ4OyrtOrcyUUB0lSb4bBEIh0roBgn66Fpojgv_PTs-GiHydJ6LSF8lpMOrzH7qZkki820r_PbvjwgN3ejJf89ZAdL3jof_gLfk49L328m-EDTQ0myANMMvm3VcAoxmNfuf_JV4GbYZWeTOCEC077Hx-x08MPX98fZWl0QubQoGjAvARfGzAFZlCyKmxjWqwFbdmItlOiqZXpVAui6joMY1YINOO28CUI6QSWreVjNgt98HuMN06CxSzNEFYeVMp2rqVzQw_GWZGLOcu2XNPDBiFDx2sywMpiwwdNXNaJy3P2jli7oyV86_hBf3mmk7loW1TQmryroVTSU4ssWJC2zb2SuEE5Zy9JMJoQLAK1yJyZ9Tjq4y8nekGIdA15ln8SfZ4QvU5EXY-SdCa9loCbJ2SsCeX-hBLt0E2W90hJtnse9R-NxW9uFefvyy92y_Sj1PYWfL8mmgpQNhiw5gwmCjdh33QlrM4jTHiDGq8gf_L_P3_K7ghq08khE2qfza4u1_4Z5llX9nk0pt8dgCXF
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Open Access Journals
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3fi9QwEA7niuCL-JNbPSWK4FOPNk07zYPIKh53wt2DunBvJUnTu8Ulrdtbufvvncl2FwN34GObSSCTmcykmX4fY--LoklbV9kkU8YlUuUy0bnO8DG1xjot8gC7eHpWHs_lt_PifI9tOVtHBQ63Hu2IT2q-Wh5e_775hA7_MbA2QLbtdNj3mgChUwUK7rH7AmMjFXmdyt29Au7YgQyVyHISyMty_JnurlGiYBUw_Xc796RfdkOUlsZFlf9EqaPH7NGYXvLZxh6esD3nn7IHG8LJm2fsZMZ998ct-SVVwXThtob3xCNMIAiYdvJfC49xjYdKc3fNF57rfjE-ac8JKZzum4fnbH709eeX42QkU0gsuhhRzktwpQadYU4li8xUusHTockr0bRKVKXSrWpAFG2Lgc0IgY7dZC4HIa3Ag2z-gk18590-45WVYDBv04SeB4UyrW3oS6IDbY1IxZQlW63V_QYzow4XZ4BnjY0eatJyPWp5yj6TaneyhHgdXnSri3p0oNpkBTQ6bUvIlXRUNAsGpGlSpyROUE7ZO1qYmjAtPBXNXOj1MNQnP87qGWHUVbTX3Cn0PRL6MAq1Ha6k1eOPCjh5wsqKJA8iSfRMGzXvk5Fs5zzUmF5mihDpqOfWcG5vfrtrpkGpEM67bk0yBeDaYAibMogMLlJf3OIXlwE4vEKLV5C-_B8tvGIPBZXvpJAIdcAmV6u1e43515V5E1zqLzUkLb8
  priority: 102
  providerName: Scholars Portal
Title A novel heteromeric pantothenate kinase complex in apicomplexan parasites
URI https://www.proquest.com/docview/2561940726
https://search.proquest.com/docview/2557228762
https://pubmed.ncbi.nlm.nih.gov/PMC8366970
https://doaj.org/article/b157da0f67394e19927b74bd0e946384
http://dx.doi.org/10.1371/journal.ppat.1009797
Volume 17
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3fa9swEBZtxmAvYz9pti54Y7AnJ7YsW9ZjWlraQULpVsibkWS5NU1lUzej_e93p8hhhu1lLwZb5xDd6XQn69N3hHxN0zKqTK7DWCgTMpGwUCYyhttIK20kTRzt4mKZnV2x76t0tUfS_iyMA-1rVU_t-m5q6xuHrWzv9KzHic0uFsd5kmWCR7N9sg_ht1-ib6dfmJRdvVOshxNyEPTn5RIez7x5pm0rkTk6ElxgET48W8kyXxamD02OwX83T4_addMNktAhhPKPmHT6irz0yWQw3_7p12TP2Dfk-ba85NNbcj4PbPPLrIMbxLw0bm8maLFqMFIeQJIZ3NYWoljgcOXmMahtINva30kbIC847i5378jV6cnP47PQl04INTgUFphn3GSSyxgyKJbGKpclrAVVktOyEjTPhKxEyWlaVRDGFKXgxmVsEk6ZprBsTd6TkW2sOSBBrhlXkKVJ5MrjqVCVLvG7oeFSKxrRMQl7rRXtliGjcNtkHFYWWz0UqPDCK3xMjlC1O1nkt3YPmvvrwlu5UHHKSxlVGU8EMwiR5YozVUZGMOggG5MvaJgCGSwsQmSu5abrivMfy2KOjHQ5ziz_FLocCH3zQlUDltTSH0uAziMz1kDycCAJfqgHzQc4SPo-dwUkk7FA_jl8sx84f2_-vGvGH0XYmzXNBmVSDraBgDUmfDDgBuobtoDXOJpw7yUf_vvNj-QFRQRPxEMqDsno4X5jPkEK9qAm4HgrPiHPjk6WF5cT9yEDrguWT5wz_gYW2DMY
link.rule.ids 230,314,727,780,784,864,885,2102,2221,12056,21388,24318,27924,27925,31719,31720,33744,33745,43310,43805,53791,53793,73745,74302
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3db9MwELegE4IXxKdWGGAQEk9hiePk4ifUoU0tbBUam7Q3y3acrWJKwrIi-O-5S92ySMBj4ksi37fty-8Ye5tlZVz5wkWJsj6SKpWRSU2Cl7GzzhuR9rCLR_N8eio_nWVnYcOtC2WVa5_YO-qycbRHvouhGdfbMYj8Q_s9oq5RdLoaWmjcZluEnJ6N2Nbe_vzL8doXo4fum59Sc5wI0jwPP8-lkOwGWb1vW0Mw0rECAn66EZx6DP-Npx61l003SEOHRZQ3otLBA3Y_pJN8spL_Q3bL14_YnVWDyV-P2WzC6-aHv-QXVPXS9KczvKW-wQR6gGkm_7aoMY7xvrLc_-SLmpt2Ea5MzQkZnDjQPWGnB_snH6dRaJ4QOTQpajEvwecGTII5lMwSW5gSV4M2LURZKVHkylSqBJFVFQYyKwQacpn4FIR0Aheu6VM2qpvabzNeOAkW8zRDaHmQKVu5knYOPRhnRSzGLFpzTbcrjAzdH5QBri1WfNDEZR24PGZ7xNoNLSFc9zeaq3MdDEbbJIPSxFUOqZKeimTBgrRl7JXECcoxe0OC0YRhUVORzLlZdp2efZ3rCWHSFeRb_kl0PCB6F4iqBiXpTPgxASdP2FgDyp0BJVqiGwxvk5Ks59zpPzqLT64V5-_DrzfD9FIqfKt9sySaDFA2GLLGDAYKN2DfcKReXPRA4QVqvIL42f8__ordnZ4cHerD2fzzc3ZPUNFODJFQO2x0fbX0LzDrurYvg2n9Bg8iKhs
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1bb9MwFLagE2gviKtWNsAgJJ5CHceJ4yfUwaqVSzUNJu3Nsh1nq5iSbFkR_HvOSZ2ySMBj6tNEPj43x1--Q8jrNC1Y6XMXxcr6SKhERCYxMVwyZ503POloF78sssMT8fE0PQ34pzbAKvuY2AXqonb4jnwCqRn220zybFIGWMTRh9m75jLCDlJ40hraadwmW5AVGR-Rrf2DxdFxH5chWneNULFRTiSTLAsf0iUynoR1e9s0BimlmZJIAnUjUXV8_puoPWou6nZQkg4BlTcy1Ow-uRdKSzpd28IDcstXD8mddbPJX4_IfEqr-oe_oOeIgKm7kxraYA9hJECAkpN-X1aQ02iHMvc_6bKiplmGK1NRZAlHDbSPycns4Nv7wyg0UogcuBe2mxfSZ0aaGOopkcY2NwXsDG2S86JUPM-UKVUheVqWkNQs5-DURewTyYXjsIlNnpBRVVd-h9DcCWmhZjPInCdTZUtX4FtEL42znPExiXqt6WbNl6G7QzMJ-4y1HjRqWQctj8k-qnYji2zX3Q_11ZkOzqNtnMrCsDKTiRIeAbPSSmEL5pWACYoxeYULo5HPokLLODOrttXzrws9RX66HOPMP4WOB0JvglBZw0o6Ez5SgMkjT9ZAcm8gCV7pBsM7aCT9nFv9x37hn73h_H345WYYb4oguMrXK5RJJawNpK8xkQODG6hvOFItzzvS8BwsXkn29P8Pf0Huglfpz_PFp12yzRG_w2TE1R4ZXV-t_DMowK7t8-BZvwEdRS5I
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+novel+heteromeric+pantothenate+kinase+complex+in+apicomplexan+parasites&rft.jtitle=PLoS+pathogens&rft.au=Tjhin%2C+Erick+T&rft.au=Howieson%2C+Vanessa+M&rft.au=Spry%2C+Christina&rft.au=van+Dooren%2C+Giel+G&rft.date=2021-07-29&rft.pub=Public+Library+of+Science&rft.issn=1553-7366&rft.eissn=1553-7374&rft.volume=17&rft.issue=7&rft_id=info:doi/10.1371%2Fjournal.ppat.1009797&rft.externalDBID=ISR&rft.externalDocID=A670983196
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1553-7374&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1553-7374&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1553-7374&client=summon