Phylogenetic analysis of the human gut microbiota using 16S rDNA clone libraries and strictly anaerobic culture-based methods

• Published in: Microbiology and immunology Vol. 46; no. 8; p. 535
• Main Authors: Hayashi, Hidenori, Sakamoto, Mitsuo, Benno, Yoshimi
• Format: Journal Article
• Language: English
• Published: Australia 01.01.2002
• Subjects: Adult; Anaerobiosis; Bacteria - classification; Bacteria - genetics; Bacteria - growth & development; Bacteria - isolation & purification; Bacteroides - classification; Bacteroides - genetics; Bacteroides - isolation & purification; Bifidobacterium - classification; Bifidobacterium - genetics; Bifidobacterium - isolation & purification; Clostridium - classification; Clostridium - genetics; Clostridium - isolation & purification; Colony Count, Microbial; Culture Media - chemistry; Cytophaga - classification; Cytophaga - genetics; Cytophaga - isolation & purification; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Feces - microbiology; Gene Library; Humans; Intestines - microbiology; Male; Middle Aged; Phylogeny; Polymerase Chain Reaction; Proteobacteria - classification; Proteobacteria - genetics; Proteobacteria - isolation & purification; RNA, Ribosomal, 16S - classification; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Streptococcus - classification; Streptococcus - genetics; Streptococcus - isolation & purification

The human gut microbiota from three healthy subjects were compared by the use of a sequence analysis of 16S rDNA libraries and a culture-based method. Direct counts ranged from 1.9 X 10" to 4.0 X 10" cells/g (wet weight), and plate counts totaled 6.6 X 10(10) to 1.2 X 10(11) CFU/g (wet wei...