Transcriptomic and morphophysiological evidence for a specialized human cortical GABAergic cell type

• Published in: Nature neuroscience Vol. 21; no. 9; pp. 1185 - 1195
• Main Authors: Boldog, Eszter, Bakken, Trygve E., Hodge, Rebecca D., Novotny, Mark, Aevermann, Brian D., Baka, Judith, Bordé, Sándor, Close, Jennie L., Diez-Fuertes, Francisco, Ding, Song-Lin, Faragó, Nóra, Kocsis, Ágnes K., Kovács, Balázs, Maltzer, Zoe, McCorrison, Jamison M., Miller, Jeremy A., Molnár, Gábor, Oláh, Gáspár, Ozsvár, Attila, Rózsa, Márton, Shehata, Soraya I., Smith, Kimberly A., Sunkin, Susan M., Tran, Danny N., Venepally, Pratap, Wall, Abby, Puskás, László G., Barzó, Pál, Steemers, Frank J., Schork, Nicholas J., Scheuermann, Richard H., Lasken, Roger S., Lein, Ed S., Tamás, Gábor
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.09.2018; Nature Publishing Group
• Subjects: 631/378/3920; 631/378/87; 9/74; Adult; Aged; Animal Genetics and Genomics; Axons - ultrastructure; Behavioral Sciences; Biological Techniques; Biomedical and Life Sciences; Biomedicine; Brain; Brain research; Cells (Biology); Cerebral Cortex - metabolism; Cerebral Cortex - ultrastructure; Cholecystokinin; Combinatorial analysis; Cortex; Cytology; Dendritic Spines - metabolism; Dendritic Spines - ultrastructure; Dendritic structure; Excavation; GABA; GABAergic Neurons - metabolism; GABAergic Neurons - ultrastructure; Gap junctions; Gap Junctions - metabolism; Gap Junctions - ultrastructure; Gene expression; Gene Library; Gene sequencing; Genes; Genomics; Glutamate decarboxylase; Humans; Information processing; Interneurons; Male; Morphology; Neural circuitry; Neurobiology; Neurons; Neurosciences; Observations; Physiology; Polymerase Chain Reaction; Presynapse; Presynaptic Terminals - metabolism; Presynaptic Terminals - ultrastructure; Properties; Pyramidal cells; Pyramidal Cells - metabolism; Pyramidal Cells - ultrastructure; Quality control; Ribonucleic acid; RNA; RNA - analysis; RNA - genetics; RNA sequencing; Rodents; Sequence Analysis, RNA; Transcriptome; γ-Aminobutyric acid

We describe convergent evidence from transcriptomics, morphology, and physiology for a specialized GABAergic neuron subtype in human cortex. Using unbiased single-nucleus RNA sequencing, we identify ten GABAergic interneuron subtypes with combinatorial gene signatures in human cortical layer 1 and c...