KRAS and Guanine Nucleotide-Binding Protein Mutations in Pancreatic Juice Collected From the Duodenum of Patients at High Risk for Neoplasia Undergoing Endoscopic Ultrasound

• Published in: Clinical gastroenterology and hepatology Vol. 13; no. 5; pp. 963 - 969.e4
• Main Authors: Eshleman, James R., Norris, Alexis L., Sadakari, Yoshihiko, Debeljak, Marija, Borges, Michael, Harrington, Colleen, Lin, Elaine, Brant, Aaron, Barkley, Thomas, Almario, J. Alejandro, Topazian, Mark, Farrell, James, Syngal, Sapna, Lee, Jeffrey H., Yu, Jun, Hruban, Ralph H., Kanda, Mitsuro, Canto, Marcia Irene, Goggins, Michael
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2015
• Subjects: Adult; Aged; Aged, 80 and over; Carrier Proteins - genetics; DNA - genetics; DNA - isolation & purification; Early Detection; EUS; Female; Gastroenterology and Hepatology; Humans; IPMN; Male; Middle Aged; Mutation; Pancreatic Juice - chemistry; Pancreatic Neoplasms - diagnosis; PDAC; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins p21(ras); ras Proteins - genetics; Screening; Sequence Analysis, DNA; Transition Temperature; Early Detection; Screening; EUS; HRM; PDAC; PanIN; GNAS; MCN; IPMN; CAPS; PCR; Cancer of the Pancreas Screening; intraductal papillary mucinous neoplasm; mucinous cystic neoplasm; endoscopic ultrasonography; guanine nucleotide-binding protein, α-stimulating; pancreatic intraepithelial neoplasia; polymerase chain reaction; high-resolution melt-curve analysis

Pancreatic imaging can identify neoplastic cysts but not microscopic neoplasms. Mutation analysis of pancreatic fluid after secretin stimulation might identify microscopic neoplasias in the pancreatic duct system. We determined the prevalence of mutations in KRAS and guanine nucleotide-binding prote...