Optimizing microbiome sequencing for small intestinal aspirates: validation of novel techniques through the REIMAGINE study - Leite, Gabriela Guimaraes Sousa; Morales, Walter; Weitsman, Stacy; Celly, Shreya; Parodi, Gonzalo; Mathur, Ruchi; Sedighi, Rashin; Barlow, Gillian M.; Rezaie, Ali; Pimentel, Mark (BMC microbiology, 2019)
Keywords: 16S rRNA gene sequencing; Agar; Anaerobes; Anaerobic microorganisms; Analysis; Bacteria; Biological Microscopy; Biomedical and Life Sciences; Chemical bonds; Colon; Consent; Deoxyribonucleic acid; Digital television; Dithiothreitol; DNA; Duodenum; Gene sequencing; Genes; Genetic research; genomics and proteomics; Gram-negative bacteria; Health; Instrument industry (Equipment); Intestinal microflora; Intestine; Klebsiella; Life Sciences; Medical instruments; Medical research; Methodology; Methodology Article; Methodology optimization; Methods; Microbial culture; Microbial genetics; Microbiology; Microbiome; Microbiomes; Microorganisms; Mucus; Mucus layer; Mycology; Novels; Optimization; Parasitology; Quantitation; RNA; rRNA 16S; Small intestine; Statistical analysis; Statistical methods; Virology; Viscosity; United States; Mucus layer; 16S rRNA gene sequencing; Microbial culture; Methodology optimization; Small intestine; Microbiome