Ultra-high resolution X-ray structures of two forms of human recombinant insulin at 100 K

• Published in: BMC chemistry Vol. 11; no. 1; p. 73
• Main Authors: Lisgarten, David R., Palmer, Rex A., Lobley, Carina M. C., Naylor, Claire E., Chowdhry, Babur Z., Al-Kurdi, Zakieh I., Badwan, Adnan A., Howlin, Brendan J., Gibbons, Nicholas C. J., Saldanha, José W., Lisgarten, John N., Basak, Ajit K.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.08.2017; Springer Nature B.V; BMC
• Subjects: Biological Chemistry; Chains; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Crystal structure; Diabetes mellitus; Electron density; Electrons; Insulin; Molecular dynamics; Research Article; Residues; Water chemistry; X-rays; Double Conformation; Minor Conformation; 2-propanol Solvent; HR Insulin; Insulin Structure

The crystal structure of a commercially available form of human recombinant (HR) insulin, Insugen (I), used in the treatment of diabetes has been determined to 0.92 Å resolution using low temperature, 100 K, synchrotron X-ray data collected at 16,000 keV (λ = 0.77 Å). Refinement carried out with ani...