高效液相色谱法同时测定保健食品中泛酸、烟酰胺、维生素B1、维生素B2、维生素B6的含量

目的建立高效液相色谱法同时测定保健食品中泛酸、烟酰胺、维生素B1、维生素B2、维生素B6的含量。方法样品经0.2%磷酸溶液超声提取,经Alltima-C18柱分离,以乙腈-5 mmol/L庚烷磺酸钠溶液为流动相进行梯度洗脱,采用二极管阵列检测器（diode array detector,DAD）同时在200 nm、280 nm波长处进行检测。结果泛酸、烟酰胺、维生素B1、维生素B2、维生素B_6能实现较好的分离,在各自线性范围内线性关系良好,平均回收率为93.9%~103.2%,相对标准偏差（relative standard deviation,RSD）为0.4%~1.8%。结论该方法操作快...

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Bibliographic Details
Published in食品安全质量检测学报 Vol. 8; no. 5; pp. 1866 - 1871
Main Author 陈彩云 魏鲜娥 蔡伟江 黎小兰 张喜金
Format Journal Article
LanguageChinese
Published 汤臣倍健股份有限公司,珠海,519040 2017
Subjects
泛酸
烟酰胺
维生素B1
维生素B2
维生素B6
高效液相色谱法
泛酸
烟酰胺
high performance liquid chromatography
vitamin B2
维生素B2
维生素B1
nicotinamide
vitamin B1
高效液相色谱法
vitamin B6
维生素B6
pantothenate
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Summary:目的建立高效液相色谱法同时测定保健食品中泛酸、烟酰胺、维生素B1、维生素B2、维生素B6的含量。方法样品经0.2%磷酸溶液超声提取,经Alltima-C18柱分离,以乙腈-5 mmol/L庚烷磺酸钠溶液为流动相进行梯度洗脱,采用二极管阵列检测器（diode array detector,DAD）同时在200 nm、280 nm波长处进行检测。结果泛酸、烟酰胺、维生素B1、维生素B2、维生素B_6能实现较好的分离,在各自线性范围内线性关系良好,平均回收率为93.9%~103.2%,相对标准偏差（relative standard deviation,RSD）为0.4%~1.8%。结论该方法操作快速简便、精密度和重复性好,可适用于保健食品中泛酸、烟酰胺、维生素B1、维生素B2、维生素B6含量的同时测定。
Bibliography:pantothenate; nicotinamide; vitamin B1; vitamin B2; vitamin B6; high performance liquid chromatography
11-5956/TS
Objective To establish a method for the simultaneous determination of pantothenate,nicotinamide,vitamin B1,vitamin B2 and vitamin B6 in healthy food by high performance liquid chromatography （HPLC）.Methods Samples were ultrasonically extracted with 0.2% phosphoric acid solution,separated by alltima-C18 column with acetonitrile-5 mmol/L sodium heptanesulfonate solution as the mobile phase for gradient elution,and then detected by diode array detector （DAD） simultaneous at the wavelengths of 200 nm and 280 nm.Results Pantothenate,nicotinamide,vitamin B1,vitamin B2 and vitamin B6 could be completely separated,and they also had good linear relationships in their linear ranges.The average recoveries were 93.9%～103.2%.The relative standard deviations were 0.4%～1.8%.Conclusion This method is rapid,simple,accurate and repeatable,which can be used for the simultaneous determination of pantothenate,nicotinami
ISSN:2095-0381