Isolation and culture of primary adult skin fibroblasts from the Asian elephant ( Elephas maximus )

• Published in: PeerJ (San Francisco, CA) Vol. 6; p. e4302
• Main Authors: Siengdee, Puntita, Klinhom, Sarisa, Thitaram, Chatchote, Nganvongpanit, Korakot
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 24.01.2018; PeerJ, Inc; PeerJ Inc
• Subjects: Asian elephant; Asiatic elephant; Biological research; Biology; Biology, Experimental; Carbon dioxide; Carcasses; Cell Biology; Cell culture; Cloning; Collagen; Collagen (type II); Collagenase; Culture; Domestication; Ear; Elephants; Elephas maximus; Environmental protection; Fetal calf serum; Fibroblasts; Freezing; Laboratories; Mammals; Medical research; Mitochondrial DNA; Physiological aspects; Public health; Research methodology; Skin; Stem cells; Studies; Tourism; Trypsin; Veterinary Medicine; Borneo; Thailand; Asia; Sri Lanka; Sumatra; Fibroblasts; Skin; Asian elephant; Culture

Primary cultures from Asian elephants ( ) allow scientists to obtain representative cells that have conserved most of their original characteristics, function, physiology and biochemistry. This technique has thus gained significant importance as a foundation for further cellular, cell biology and mo...