Multiple microRNAs modulate p21Cip1 Waf1 expression by directly targeting its 3′ untranslated region

• Published in: Oncogene Vol. 29; no. 15; pp. 2302 - 2308
• Main Authors: Wu, S, Huang, S, Ding, J, Zhao, Y, Liang, L, Liu, T, Zhan, R, He, X
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.04.2010; Nature Publishing Group
• Subjects: 3' Untranslated regions; 3' Untranslated Regions - genetics; 631/208/200; 631/337/384/331; 631/67; Apoptosis; Base Sequence; Biological and medical sciences; Cancer; Cell Biology; Cell Cycle - genetics; Cell Line; Cell physiology; Cell Proliferation; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Choriocarcinoma; Chromosome 19; Chromosomes, Human, Pair 19 - genetics; Cyclin-dependent kinase; Cyclin-dependent kinase inhibitor p21; Cyclin-Dependent Kinase Inhibitor p21 - genetics; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Cyclin-dependent kinases; DNA Mutational Analysis; Enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Genes, Reporter - genetics; Genetic aspects; Genetic regulation; Genetics; High-Throughput Screening Assays; Human Genetics; Humans; Internal Medicine; Kinases; Luciferases - genetics; Medicine; Medicine & Public Health; MicroRNA; MicroRNAs; MicroRNAs - genetics; miRNA; Molecular and cellular biology; mRNA; Multigene Family; Mutation; Oncology; Physiological aspects; Ribonucleic acid; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; short-communication; Tumor suppressor genes; Tumorigenesis; Tumors; China; miRNA; p21Cip1/Waf1; 3′ untranslated region; 3' untranslated region; Gene silencing; RNA interference; Targeting; CDKN1A Gene; Micro RNA; Carcinogenesis; Cyclin dependent kinase inhibitor

Cyclin-dependent kinase inhibitor 1A (CDKN1A), also known as p21Cip1/Waf1, is a master downstream effector of tumor suppressors. In this study, we experimentally demonstrate through a high-throughput luciferase reporter screen that p21Cip1/Waf1 can be directly targeted by nearly 28 microRNAs (miRNAs...