超高压处理对养殖大黄鱼肉蛋白质特性的影响

目的研究超高压处理对养殖大黄鱼肉蛋白质特性的影响。方法超高压处理养殖大黄鱼肉后,通过双缩脲法、十二烷基硫酸钠-聚丙烯酰氨凝胶电泳（sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE）、差示扫描量热法（differential scanning calorimetry,DSC）及蛋白质巯基含量及持水性分析,研究超高压对养殖大黄鱼肉品质的影响。结果压强对不同类型溶解性的蛋白含量有较大影响。350 MPa 10 min时水溶性蛋白含量最高,达到74.12 mg/g;300 MPa 25 min时酸溶性蛋白含量最高,达到...

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Published in食品安全质量检测学报 Vol. 8; no. 2; pp. 579 - 586
Main Author 陈世达 朱艳杰 张登科 杨华 吴林洁
Format Journal Article
LanguageChinese
Published 浙江万里学院生物与环境学院,宁波,315100%宁波大学海洋学院,宁波,315211%浙江万里学院生物与环境学院,宁波 315100 2017
宁波大学海洋学院,宁波 315211
Subjects
养殖大黄鱼
影响因素
蛋白质
超高压
influencing factors
影响因素
protein
cultured Pseudosciaena crocea
养殖大黄鱼
ultrahigh pressure
超高压
蛋白质
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ISSN2095-0381

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Summary:目的研究超高压处理对养殖大黄鱼肉蛋白质特性的影响。方法超高压处理养殖大黄鱼肉后,通过双缩脲法、十二烷基硫酸钠-聚丙烯酰氨凝胶电泳（sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE）、差示扫描量热法（differential scanning calorimetry,DSC）及蛋白质巯基含量及持水性分析,研究超高压对养殖大黄鱼肉品质的影响。结果压强对不同类型溶解性的蛋白含量有较大影响。350 MPa 10 min时水溶性蛋白含量最高,达到74.12 mg/g;300 MPa 25 min时酸溶性蛋白含量最高,达到14.53 mg/g。可见超高压处理显著改变了养殖大黄鱼不同溶解性、不同结构蛋白的含量（P〈0.05）;提高了养殖大黄鱼蛋白质的热稳定性;增加了蛋白质的巯基含量;降低了蛋白质的持水能力。结论超高压处理使养殖大黄鱼肉蛋白质的持水性降低,溶解性、巯基含量及热稳定性增加,并使蛋白质结构发生改变。
Bibliography:CHEN Shi-Da1, ZHU Yan-Jiel, ZHANG Deng-Ke2, YANG Hua1,2, WU Lin-Jie1 （1. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China; 2. School of Marine Sciences, Ningbo University, Ningbo 315211, China）
ultrahigh pressure;cultured Pseudosciaena crocea;protein;influencing factors
11-5956/TS
Objective To investigate the effects of ultrahigh pressure（UHP） on protein characteristics of cultured Pseudosciaena crocea meat. Methods The protein content, thiol content and water binding capacity of cultured Pseudosciaena crocea protein were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE） and differential scanning calorimetry（DSC）, so as to investigate the effects of UHP on protein characteristics of cultured Pseudosciaena crocea meat. Results Pressure had a great influence on the protein content of different types of solubility. Water soluble protein content reached to 74.12 mg/g at 350 MPa for 10 min, and the highest acid soluble protein content was
ISSN:2095-0381