玉米细菌性枯萎病菌EGase内切葡聚糖酶蛋白纯化研究初探
目的研究玉米细菌性枯萎病菌EGase内切葡聚糖酶蛋白纯化的方法。方法以玉米细菌性枯萎病菌（Pantoea stewartii subsp.stewartii）菌株为材料,分离纯化其内切葡聚糖酶Egase。制备EGase浓缩液,浓缩液经SephradexTMG-75凝胶过滤层析和DEAE-Sephorose Fast Flow阴离子交换柱层析等提纯步骤,获得了凝胶电泳均一的内切葡聚糖酶。结果经变性聚丙烯酰胺凝胶电泳检测为一条电泳带,纯化后的EGase是单体蛋白,分子量约为72.3 k Da。Egase酶反应的最适温度是60℃,最适pH为5.0。结论本研究从玉米细菌性枯萎病菌中分离得到了一种新的内...
Saved in:
| Published in | 食品安全质量检测学报 Vol. 7; no. 4; pp. 1392 - 1396 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
山东出入境检验检疫局检验检疫技术中心,青岛,266002
2016
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
Cover
| Abstract | 目的研究玉米细菌性枯萎病菌EGase内切葡聚糖酶蛋白纯化的方法。方法以玉米细菌性枯萎病菌（Pantoea stewartii subsp.stewartii）菌株为材料,分离纯化其内切葡聚糖酶Egase。制备EGase浓缩液,浓缩液经SephradexTMG-75凝胶过滤层析和DEAE-Sephorose Fast Flow阴离子交换柱层析等提纯步骤,获得了凝胶电泳均一的内切葡聚糖酶。结果经变性聚丙烯酰胺凝胶电泳检测为一条电泳带,纯化后的EGase是单体蛋白,分子量约为72.3 k Da。Egase酶反应的最适温度是60℃,最适pH为5.0。结论本研究从玉米细菌性枯萎病菌中分离得到了一种新的内切葡聚合糖酶,对其部分性质进行了表述,为后续EGase基因的克隆及表达研究提供了研究基础。 |
|---|---|
| AbstractList | 目的 研究玉米细菌性枯萎病菌EGase内切葡聚糖酶蛋白纯化的方法.方法 以玉米细菌性枯萎病菌(Pantoea stewartii subsp.stewartii)菌株为材料,分离纯化其内切葡聚糖酶Egase.制备EGase浓缩液,浓缩液经SephradexTM G-75凝胶过滤层析和DEAE-Sephorose Fast Flow阴离子交换柱层析等提纯步骤,获得了凝胶电泳均一的内切葡聚糖酶.结果 经变性聚丙烯酰胺凝胶电泳检测为一条电泳带,纯化后的EGase是单体蛋白,分子量约为72.3 kDa.Egase酶反应的最适温度是60℃,最适pH为5.0.结论 本研究从玉米细菌性枯萎病菌中分离得到了一种新的内切葡聚合糖酶,对其部分性质进行了表述,为后续EGase基因的克隆及表达研究提供了研究基础. 目的研究玉米细菌性枯萎病菌EGase内切葡聚糖酶蛋白纯化的方法。方法以玉米细菌性枯萎病菌（Pantoea stewartii subsp.stewartii）菌株为材料,分离纯化其内切葡聚糖酶Egase。制备EGase浓缩液,浓缩液经SephradexTMG-75凝胶过滤层析和DEAE-Sephorose Fast Flow阴离子交换柱层析等提纯步骤,获得了凝胶电泳均一的内切葡聚糖酶。结果经变性聚丙烯酰胺凝胶电泳检测为一条电泳带,纯化后的EGase是单体蛋白,分子量约为72.3 k Da。Egase酶反应的最适温度是60℃,最适pH为5.0。结论本研究从玉米细菌性枯萎病菌中分离得到了一种新的内切葡聚合糖酶,对其部分性质进行了表述,为后续EGase基因的克隆及表达研究提供了研究基础。 |
| Author | 厉艳 邵秀玲 张京宣 王英超 封立平 王简 |
| AuthorAffiliation | 山东出入境检验检疫局检验检疫技术中心,青岛266002 |
| AuthorAffiliation_xml | – name: 山东出入境检验检疫局检验检疫技术中心,青岛,266002 |
| Author_FL | SHAO Xiu-Ling WANG Ying-Chao WANG Jian FENG Li-Ping LI Yan ZHANG Jing-Xuan |
| Author_FL_xml | – sequence: 1 fullname: LI Yan – sequence: 2 fullname: SHAO Xiu-Ling – sequence: 3 fullname: ZHANG Jing-Xuan – sequence: 4 fullname: WANG Ying-Chao – sequence: 5 fullname: FENG Li-Ping – sequence: 6 fullname: WANG Jian |
| Author_xml | – sequence: 1 fullname: 厉艳 邵秀玲 张京宣 王英超 封立平 王简 |
| BookMark | eNotzb9Lw0AcBfAbKlhr_wlXCXxzyV3uRim1KgWX7uGS3tWUmv4IIjrp0FppiQpSUVHURZyKFDoo_jnpxf_CQJ0eDz68t4ZyYTuUOZTHwIkBFjNXUTGKAg_AMTGnmObRno4v9edMfw_Sq_Hi7H3xPE2vY33Xz2q5IiKZDPrJ8CK9eU3PH_Rs8tufp48jff-jv6bJeKJfbvXHPBk-LeK3dbSiRCuSxf8soNp2uVbaMar7ld3SVtXwqUUNQoktfcBSOIxLT_lCMAlgcmnWFRDAxPOEIFyCksRjFLDNbFxXCktHmhZYBbS5nD0WoRJhw222j3phduhGHdE9bTX9ZoTBpGAD0ExvLLV_0A4b3SDznV5wKHonLqWMWxxzbP0BsAlvrQ |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W92 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DatabaseName | 维普_期刊 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-工程技术 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Diet & Clinical Nutrition |
| DocumentTitleAlternate | Primary studies of endoglucanase purified from Pantoea stewartii subsp.stewartii |
| DocumentTitle_FL | Primary studies of endoglucanase purified from Pantoea stewartii subsp.stewartii |
| EndPage | 1396 |
| ExternalDocumentID | spaqzljcjs201604006 668939292 |
| GrantInformation_xml | – fundername: 国家质检总局科技计划项目(2013IK293,2014IK021,2015IK213)Supported by the National Scientific and Technological Project of the General Administration of Quality Supervision funderid: (2013IK293,2014IK021,2015IK213) |
| GroupedDBID | -02 2B. 2C0 2RA 92H 92I 92L ALMA_UNASSIGNED_HOLDINGS CCEZO CDRFL CQIGP C~G DYU OK1 OZF TCJ TGT U1G U5L W92 ~WA 4A8 93N PSX |
| ID | FETCH-LOGICAL-c636-5654ec02ea789ebfcaa8e0019e1df05025bbaa59e0fe5b86024842dff2e7e1303 |
| ISSN | 2095-0381 |
| IngestDate | Thu May 29 04:00:39 EDT 2025 Wed Feb 14 10:19:00 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Keywords | endoglucanase protein purification 蛋白纯化 内切葡聚糖酶 玉米细菌性枯萎病菌 Pantoea stewartii subsp.stewartii |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c636-5654ec02ea789ebfcaa8e0019e1df05025bbaa59e0fe5b86024842dff2e7e1303 |
| Notes | 11-5956/TS Pantoea stewartii subsp.stewartii; endoglucanase; protein purification LI Yan;SHAO Xiu-Ling;ZHANG Jing-Xuan;WANG Ying-Chao;FENG Li-Ping;WANG Jian;Technical Center of Shandong Entry-Exit Inspection and Quarantine Bureau; Qingdao 266002, China Objective To investigate the purification method of endoglucanase（EGase） from Pantoea stewartii subsp. stewartii. Methods With the material of Pantoea stewartii subsp. stewartii. strain, EGase fermented liquid was prepared, and then eluted using Sephadex~（TM）G-75 and on DEAE-Sepharose Fast Flow anion exchange. SDS-PAGE was used to determine the EGase activity after purification. Results Endoglucanase was purified from Pantoea stewartii subsp. stewartii. It was monomer protein and its molecular size was 72.3 k Da. The optimum reaction temperature of endoglucanase was 60 ℃ and the optimum pH was 5.0. Conclusion Endoglucanase was purified firstly from Pantoea stewartii subsp. stewartii and its properties were described. This study will provide an important base for c |
| PageCount | 5 |
| ParticipantIDs | wanfang_journals_spaqzljcjs201604006 chongqing_primary_668939292 |
| PublicationCentury | 2000 |
| PublicationDate | 2016 |
| PublicationDateYYYYMMDD | 2016-01-01 |
| PublicationDate_xml | – year: 2016 text: 2016 |
| PublicationDecade | 2010 |
| PublicationTitle | 食品安全质量检测学报 |
| PublicationTitleAlternate | Journal of Food Safety and Quality |
| PublicationTitle_FL | Journal of Food Safety & Quality |
| PublicationYear | 2016 |
| Publisher | 山东出入境检验检疫局检验检疫技术中心,青岛,266002 |
| Publisher_xml | – name: 山东出入境检验检疫局检验检疫技术中心,青岛,266002 |
| SSID | ssib007129626 ssib017479571 ssib051376490 ssib007891667 ssj0001257730 |
| Score | 2.0199866 |
| Snippet | 目的研究玉米细菌性枯萎病菌EGase内切葡聚糖酶蛋白纯化的方法。方法以玉米细菌性枯萎病菌（Pantoea stewartii subsp.stewartii）菌株为材料,分离纯化其内切葡聚糖酶Egase。制备EGase浓缩液,浓缩液经SephradexTMG-75凝胶过滤层析和DEAE-Sephorose Fast... 目的 研究玉米细菌性枯萎病菌EGase内切葡聚糖酶蛋白纯化的方法.方法 以玉米细菌性枯萎病菌(Pantoea stewartii subsp.stewartii)菌株为材料,分离纯化其内切葡聚糖酶Egase.制备EGase浓缩液,浓缩液经SephradexTM G-75凝胶过滤层析和DEAE-Sephorose... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 1392 |
| SubjectTerms | 内切葡聚糖酶 玉米细菌性枯萎病菌 蛋白纯化 |
| Title | 玉米细菌性枯萎病菌EGase内切葡聚糖酶蛋白纯化研究初探 |
| URI | http://lib.cqvip.com/qk/71822X/201604/668939292.html https://d.wanfangdata.com.cn/periodical/spaqzljcjs201604006 |
| Volume | 7 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Na9RAFA-1J0HET6xV2UPHS1nJ12Rmjpnd1FKwpxbqaUmyk9ZStq3dXnrSQ2vFUhWkoqKoF_FUpNCD4p-zzfpf-N5Lug0iol7C2-S933szbzLzm2wmY1ljAVAOkULjlbGACUoKHE4Zk9Qd13htGafQBeLa4TvTweSsPzXH54ZOnam8tbTeTW6lG79dV_I_WYVzkFdcJfsPmR2AwgmQIb9whAzD8a9yzCLBZMSkQkE7THskaCYDFkkmJ5hssCjAtxlCgYKKWDiBl5SNhqCsAIEPlKPbMKixiCMAngYBrgiycFjokKLDVEh-PKbAj0JNTQ4VeNaEqphukk5IDjkGgsqChTZTPgmKrMiFalKYEJ1bZcsIHoIXQlAeugYhLErM0W8o0a_2SVAYKpQDoMAKCg2C5hQSaDZZGJAXCIkftzOCKfAkHrU3TjguGmKQgnComrU7juq6gWWIfCpbowwoRLsiGZqgNOYDQ8OKLOxsih8wdRmSLv0N7ASVza4-iSmWiBZdtWvjOnev2HymvGP8yrAANNutUAz4-cvnv4lQBAGQR6CryCiAN-ILis27s4PeUQA1CypkWUi4z07II0wtheIn5J47MHb45X-gxRNHLgRtvjMIFz8osrDcmV8FmkSr1jpZ3JmvEKyZc9bZcmZUC4tmft4a2li4YI0075lu7Wat_HztUm36ePeIi9ZUvvs4_3KQf9vqP905evDp6N1-_9lu_nITflJD7m1t9rYf9Z9_6D98nR_s_dg87L95kr_6nn_d7-3s5e9f5J8Pe9tvj3Y_XrJmJqKZxmS93BqkngZeUIdZiG9S2zUxVIJJsjSOpcHZinHamc2BxydJHHNl7MzwBLdZ86XvtrPMNcIga7tsDXeWO-aKVXOhMjwnlVy2lZ8FqTRtmcXGi2UbhieXj1ijgypqrRRfgGkNMjVijZWV1ir7hbUWpHx1Y2kxXVzDRoKDZHD1jyCj1mnULB7sXbOGu_fXzXWgut3kBrWAn8pmgZ0 |
| linkProvider | CAB International |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E7%8E%89%E7%B1%B3%E7%BB%86%E8%8F%8C%E6%80%A7%E6%9E%AF%E8%90%8E%E7%97%85%E8%8F%8CEGase%E5%86%85%E5%88%87%E8%91%A1%E8%81%9A%E7%B3%96%E9%85%B6%E8%9B%8B%E7%99%BD%E7%BA%AF%E5%8C%96%E7%A0%94%E7%A9%B6%E5%88%9D%E6%8E%A2&rft.jtitle=%E9%A3%9F%E5%93%81%E5%AE%89%E5%85%A8%E8%B4%A8%E9%87%8F%E6%A3%80%E6%B5%8B%E5%AD%A6%E6%8A%A5&rft.au=%E5%8E%89%E8%89%B3+%E9%82%B5%E7%A7%80%E7%8E%B2+%E5%BC%A0%E4%BA%AC%E5%AE%A3+%E7%8E%8B%E8%8B%B1%E8%B6%85+%E5%B0%81%E7%AB%8B%E5%B9%B3+%E7%8E%8B%E7%AE%80&rft.date=2016&rft.issn=2095-0381&rft.issue=4&rft.spage=1392&rft.epage=1396&rft.externalDocID=668939292 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F71822X%2F71822X.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fspaqzljcjs%2Fspaqzljcjs.jpg |