Sirolimus and post transplant Cy synergistically maintain mixed chimerism in a mismatched murine model

• Published in: Bone marrow transplantation (Basingstoke) Vol. 48; no. 10; pp. 1335 - 1341
• Main Authors: Fitzhugh, C D, Weitzel, R P, Hsieh, M M, Phang, O A, Madison, C, Luznik, L, Powell, J D, Tisdale, J F
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.10.2013; Nature Publishing Group
• Subjects: 631/250/1904; 692/699/1541; 692/700/565/251/1574; Analysis; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animal models; Animals; Biocompatibility; Biological and medical sciences; Blood diseases; Bone marrow; Bone marrow, stem cells transplantation. Graft versus host reaction; Care and treatment; Cell Biology; Chimeras (Organisms); Chimerism; Conditioning; Cyclophosphamide - pharmacology; Disease Models, Animal; Dosage and administration; Drug Synergism; Female; Hematological diseases; Hematology; Histocompatibility antigen HLA; Immunosuppressive Agents - pharmacology; Internal Medicine; Medical sciences; Medicine; Medicine & Public Health; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred NOD; original-article; Patients; Phenotypes; Physiological aspects; Public Health; Rapamycin; Sickle cell disease; Sirolimus - pharmacology; Skin Transplantation - methods; Stem cell transplantation; Stem Cells; Toxicity; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transplantation Chimera; CSA; murine; mixed chimerism; Cy; sirolimus; Antineoplastic agent; Incompatibility; Animal model; Hematology; Sirolimus; Rodentia; Lactone; Macrolide; Macrocycle; Vertebrata; Antibiotic; Mammalia; Mouse; Mixed; Animal; Protein synthesis inhibitor; Chimerism; Immunosuppressive agent

Because of the toxicity associated with myeloablative conditioning, nonmyeloablative regimens are increasingly being used in vulnerable patient populations. For patients with sickle cell disease, stable mixed chimerism has proven sufficient to reverse the phenotype. Because the vast majority of pati...