Hairpin-end conformation of adeno-associated virus genome determines interactions with DNA-repair pathways

• Published in: Gene therapy Vol. 20; no. 6; pp. 686 - 693
• Main Authors: Cataldi, M P, McCarty, D M
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.06.2013; Nature Publishing Group
• Subjects: 631/1647/2300; 631/326/596/2561; 631/337/1427/2190; Adeno-associated virus; Ataxia telangiectasia; Ataxia telangiectasia mutated protein; Biomedical and Life Sciences; Biomedicine; Cell Biology; Conformation; Dependovirus - genetics; Dependoviruses; DNA repair; DNA Repair - genetics; DNA Replication - genetics; DNA viruses; DNA, Viral - chemistry; DNA, Viral - genetics; Gene Expression; Gene silencing; Gene Therapy; Genetic aspects; Genetic Vectors; Genome, Viral; Genomes; Health aspects; Human Genetics; Humans; Inverted Repeat Sequences - genetics; Metabolic Networks and Pathways - genetics; Nanotechnology; Nucleotide sequence; original-article; Recombination; Risk factors; Single-stranded DNA; Terminal Repeat Sequences - genetics; Virion - genetics; Virion - growth & development; Virions; Virus diseases; Viruses; United States; hairpin; adeno-associated virus (AAV); terminal repeat (TR); ataxia telangiectasia mutate (ATM); silencing

The palindromic terminal repeats (TRs) of adeno-associated virus (AAV) form DNA hairpins (HPs) are essential for replication and for priming the conversion of single-stranded virion DNA to double strand. In recombinant AAV (rAAV) gene-delivery vectors, they are targets for the DNA-repair pathways le...