肽核酸荧光原位杂交技术快速检测食品中的李斯特菌属及单增李斯特菌
目的建立利用肽核酸荧光原位杂交技术（PNA-FISH）快速检测食品中李斯特菌属及单增李斯特菌的方法。方法针对李斯特菌属、单增李斯特菌分别设计合成2份PNA探针lis-16S-1、lm-16S-2,并建立荧光原位杂交技术,优化杂交条件,对选取的13株李斯特菌和其他9株非李斯特菌进行检测,验证探针的特异性和灵敏度,并对118份食品样品用LB肉汤2次增菌培养后进行PNA-FISH检测。结果探针灵敏度和特异性均为100%,从118份食品中检出14株李斯特菌和8株单增李斯特菌,结果与API方法和VITEK方法鉴定结果一致。结论 PNA-FISH方法可靠易行,对从食品中检测致病性单增李斯特菌有较高的实用性...
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| Published in | 食品安全质量检测学报 Vol. 7; no. 4; pp. 1387 - 1391 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
烟台出入境检验检疫局,烟台,264000
2016
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
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| Abstract | 目的建立利用肽核酸荧光原位杂交技术（PNA-FISH）快速检测食品中李斯特菌属及单增李斯特菌的方法。方法针对李斯特菌属、单增李斯特菌分别设计合成2份PNA探针lis-16S-1、lm-16S-2,并建立荧光原位杂交技术,优化杂交条件,对选取的13株李斯特菌和其他9株非李斯特菌进行检测,验证探针的特异性和灵敏度,并对118份食品样品用LB肉汤2次增菌培养后进行PNA-FISH检测。结果探针灵敏度和特异性均为100%,从118份食品中检出14株李斯特菌和8株单增李斯特菌,结果与API方法和VITEK方法鉴定结果一致。结论 PNA-FISH方法可靠易行,对从食品中检测致病性单增李斯特菌有较高的实用性。 |
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| AbstractList | 目的建立利用肽核酸荧光原位杂交技术（PNA-FISH）快速检测食品中李斯特菌属及单增李斯特菌的方法。方法针对李斯特菌属、单增李斯特菌分别设计合成2份PNA探针lis-16S-1、lm-16S-2,并建立荧光原位杂交技术,优化杂交条件,对选取的13株李斯特菌和其他9株非李斯特菌进行检测,验证探针的特异性和灵敏度,并对118份食品样品用LB肉汤2次增菌培养后进行PNA-FISH检测。结果探针灵敏度和特异性均为100%,从118份食品中检出14株李斯特菌和8株单增李斯特菌,结果与API方法和VITEK方法鉴定结果一致。结论 PNA-FISH方法可靠易行,对从食品中检测致病性单增李斯特菌有较高的实用性。 目的 建立利用肽核酸荧光原位杂交技术(PNA-FISH)快速检测食品中李斯特菌属及单增李斯特菌的方法.方法 针对李斯特菌属、单增李斯特菌分别设计合成2份PNA探针lis-16S-1、1m-16S-2,并建立荧光原位杂交技术,优化杂交条件,对选取的1 3株李斯特菌和其他9株非李斯特菌进行检测,验证探针的特异性和灵敏度,并对118份食品样品用LB肉汤2次增菌培养后进行PNA-FISH检测.结果 探针灵敏度和特异性均为100％,从118份食品中检出14株李斯特菌和8株单增李斯特菌,结果与API方法和VITEK方法鉴定结果一致.结论 PNA-FISH方法可靠易行,对从食品中检测致病性单增李斯特菌有较高的实用性. |
| Author | 王云霞 崔艳梅 刘宁 牟妍 段效辉 |
| AuthorAffiliation | 烟台出入境检验检疫局,烟台264000 |
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| Author_FL | LIU Ning CUI Yan-Mei MOU Yan DUAN Xiao-Hui WANG Yun-Xia |
| Author_FL_xml | – sequence: 1 fullname: WANG Yun-Xia – sequence: 2 fullname: CUI Yan-Mei – sequence: 3 fullname: LIU Ning – sequence: 4 fullname: MOU Yan – sequence: 5 fullname: DUAN Xiao-Hui |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Determination of Listeria genus and Listeria monocytogenes in foods by peptide nucleic acid-fluorescence in situ hybridization |
| DocumentTitle_FL | Determination of Listeria genus and Listeria monocytogenes in foods by peptide nucleic acid-fluorescence in situ hybridization |
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| GrantInformation_xml | – fundername: 质检总局科技计划项目; 山东检验检疫局科技计划项目(SK201419)Supported by the Scientific and Technological Project of the General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China; the Scientific and Technological Project of Shandong Exit-Entry Inspection and Quarantine Bureau funderid: (2015IK195,2015IK200); (2015IK195,2015IK200); (SK201419) |
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| Keywords | fluorescence in situ hybridization Listeria genus,Listeria monocytogenes 荧光原位杂交技术 李斯特菌 肽核酸 单增李斯特菌 peptide nucleic acid |
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| Notes | 11-5956/TS WANG Yun-Xia;CUI Yan-Mei;LIU Ning;MOU Yan;DUAN Xiao-Hui;Yantai Entry-Exit Inspection and Quarantine Bureau; Yantai 264000, China peptide nucleic acid; fluorescence in situ hybridization; Listeria genus; Listeria monocytogenes Objective To develop a method of fluorescence in situ hybridization（FISH） using peptide nucleic acid（PNA） probes for the rapid detection of Listeria genus and Listeria monocytogenes in foodstuffs. Method Two PNA probes of lis-16S-1 and lm-16S-2 were synthesized for specific identification of Listeria genus and Listeria monocytogenes respectively, the PNA-FISH method was developed, and then the optimal reaction conditions were definitely explored. Thirteen strains of Listeria and 9 other bacterial species were detected to evaluate the sensitivity and specificity of the method, and 118 foodstuffs were detected using PNA-FISH after cultured by LB broth for 2 times. Results The sensitivity and specificity of PNA probes were both 100%. Fourteen strains of Listeria and 8 strains of Lis |
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| PublicationTitle | 食品安全质量检测学报 |
| PublicationTitleAlternate | Journal of Food Safety and Quality |
| PublicationTitle_FL | Journal of Food Safety & Quality |
| PublicationYear | 2016 |
| Publisher | 烟台出入境检验检疫局,烟台,264000 |
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| Snippet | ... 目的 建立利用肽核酸荧光原位杂交技术(PNA-FISH)快速检测食品中李斯特菌属及单增李斯特菌的方法.方法 针对李斯特菌属、单增李斯特菌分别设计合成2份PNA探针lis-16S-1、1m-16S-2,并建立荧光原位杂交技术,优化杂交条件,对选取的1... |
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| StartPage | 1387 |
| SubjectTerms | 单增李斯特菌 李斯特菌 肽核酸 荧光原位杂交技术 |
| Title | 肽核酸荧光原位杂交技术快速检测食品中的李斯特菌属及单增李斯特菌 |
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