Structural definition of a neutralization epitope on the N-terminal domain of MERS-CoV spike glycoprotein

• Published in: Nature communications Vol. 10; no. 1; pp. 3068 - 13
• Main Authors: Zhou, Haixia, Chen, Yingzhu, Zhang, Shuyuan, Niu, Peihua, Qin, Kun, Jia, Wenxu, Huang, Baoying, Zhang, Senyan, Lan, Jun, Zhang, Linqi, Tan, Wenjie, Wang, Xinquan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11.07.2019; Nature Publishing Group; Nature Portfolio
• Subjects: 38/70; 59/5; 631/326/596; 631/535/1266; 64/110; 64/60; 82/1; 82/103; 82/16; 82/80; 82/83; Animals; Antibodies, Neutralizing - blood; Antibodies, Neutralizing - immunology; Antibodies, Neutralizing - metabolism; Antibodies, Neutralizing - ultrastructure; Antibodies, Viral - blood; Antibodies, Viral - immunology; Antibodies, Viral - metabolism; Antibodies, Viral - ultrastructure; Binding; Cell adhesion; Cell Line, Tumor; Chlorocebus aethiops; Coronaviridae; Coronavirus Infections - blood; Coronavirus Infections - immunology; Coronavirus Infections - virology; Coronaviruses; Crystallography, X-Ray; Dipeptidyl Peptidase 4 - metabolism; Dipeptidyl-peptidase IV; Disease Models, Animal; Epitope Mapping; Epitopes; Epitopes - immunology; Female; Glycoproteins; HEK293 Cells; Humanities and Social Sciences; Humans; Mice; Middle East Respiratory Syndrome Coronavirus - immunology; Middle East Respiratory Syndrome Coronavirus - metabolism; Monoclonal antibodies; multidisciplinary; Mutagenesis; Neutralization; Neutralization Tests; Peptidase; Protein Binding - immunology; Protein Domains - immunology; Recombinant Proteins - immunology; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Recombinant Proteins - ultrastructure; Respiratory diseases; Science; Science (multidisciplinary); Spike glycoprotein; Spike Glycoprotein, Coronavirus - immunology; Spike Glycoprotein, Coronavirus - isolation & purification; Spike Glycoprotein, Coronavirus - metabolism; Spike Glycoprotein, Coronavirus - ultrastructure; Synergistic effect; Vero Cells; Virus Internalization

Most neutralizing antibodies against Middle East respiratory syndrome coronavirus (MERS-CoV) target the receptor-binding domain (RBD) of the spike glycoprotein and block its binding to the cellular receptor dipeptidyl peptidase 4 (DPP4). The epitopes and mechanisms of mAbs targeting non-RBD regions...