Genome-scale single-cell mechanical phenotyping reveals disease-related genes involved in mitotic rounding

• Published in: Nature communications Vol. 8; no. 1; pp. 1266 - 11
• Main Authors: Toyoda, Yusuke, Cattin, Cedric J., Stewart, Martin P., Poser, Ina, Theis, Mirko, Kurzchalia, Teymuras V., Buchholz, Frank, Hyman, Anthony A., Müller, Daniel J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02.11.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647/2163; 631/57; 631/80/641/1655; 639/766/747; 639/925/930; Actomyosin; Cell division; Cell size; Confinement; Contractility; Endoplasmic reticulum; Genes; Genomes; Humanities and Social Sciences; Intracellular; Metaphase; Mitochondria; Mitosis; multidisciplinary; Myosin; PARK7 protein; Phenotypes; Phenotyping; Pressure; Proteins; RNA-mediated interference; Rounding; Science; Science (multidisciplinary); Yeast

To divide, most animal cells drastically change shape and round up against extracellular confinement. Mitotic cells facilitate this process by generating intracellular pressure, which the contractile actomyosin cortex directs into shape. Here, we introduce a genome-scale microcantilever- and RNAi-ba...