Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages

• Published in: Nature communications Vol. 11; no. 1; pp. 1711 - 14
• Main Authors: Rosenbluth, Jennifer M., Schackmann, Ron C. J., Gray, G. Kenneth, Selfors, Laura M., Li, Carman Man-Chung, Boedicker, Mackenzie, Kuiken, Hendrik J., Richardson, Andrea, Brock, Jane, Garber, Judy, Dillon, Deborah, Sachs, Norman, Clevers, Hans, Brugge, Joan S.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06.04.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/106; 13/31; 14; 14/19; 631/1647/767; 631/532/2436; 692/420/755; Adult; BRCA1 Protein - genetics; Breast cancer; Breast Neoplasms; Cell culture; Cell Culture Techniques - methods; Cell differentiation; Cell Differentiation - genetics; Cell Differentiation - physiology; Cell Lineage - genetics; Cytometry; Epidermal Growth Factor - pharmacology; Epithelial cells; ErbB Receptors - metabolism; Female; Health risks; Humanities and Social Sciences; Humans; Mammary gland; Mammary Glands, Human - chemistry; Mammary Glands, Human - cytology; Mammary Glands, Human - metabolism; Middle Aged; multidisciplinary; Organoids; Organoids - chemistry; Organoids - cytology; Organoids - metabolism; p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors; Progenitor cells; Propagation; Science; Science (multidisciplinary); Single-Cell Analysis; Stem cells; Stem Cells - chemistry; Stem Cells - cytology; Stem Cells - metabolism; Tissues; Transforming Growth Factor beta - antagonists & inhibitors; Young Adult

Recently, organoid technology has been used to generate a large repository of breast cancer organoids. Here we present an extensive evaluation of the ability of organoid culture technology to preserve complex stem/progenitor and differentiated cell types via long-term propagation of normal human mam...