Microarray Analysis and Barcoded Pyrosequencing Provide Consistent Microbial Profiles Depending on the Source of Human Intestinal Samples

• Published in: Applied and Environmental Microbiology Vol. 77; no. 6; pp. 2071 - 2080
• Main Authors: van den Bogert, Bartholomeus, de Vos, Willem M, Zoetendal, Erwin G, Kleerebezem, Michiel
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.03.2011; American Society for Microbiology (ASM)
• Subjects: Actinobacteria; Bacteria; bacterial community; Biological and medical sciences; Cloning; Comparative analysis; DNA; DNA Barcoding, Taxonomic - methods; DNA sequencing; effluents; extensive set; fecal samples; feces composition; Fundamental and applied biological sciences. Psychology; gastrointestinal-tract microbiota; genes; gut microbiome; Human subjects; Humans; intestinal microorganisms; Intestines - microbiology; Metagenome - genetics; Methods; microarray technology; Microbial activity; microbial communities; Microbiology; Molecular Sequence Data; nucleotide sequences; Oligonucleotide Array Sequence Analysis - methods; phylogenetic microarray; Phylogenetics; phylogeny; Polymerase chain reaction; quantitative-analysis; real-time pcr; ribosomal RNA; ribosomal-rna genes; RNA, Ribosomal, 16S - genetics; Sample preparation; sequence data; Small intestine; Species diversity; Species richness; Studies; Veillonella; Human; Microorganism; Microarray; Sample; Gut

Large-scale and in-depth characterization of the intestinal microbiota necessitates application of high-throughput 16S rRNA gene-based technologies, such as barcoded pyrosequencing and phylogenetic microarray analysis. In this study, the two techniques were compared and contrasted for analysis of th...