A Wolbachia wMel transinfection in Aedes albopictus is not detrimental to host fitness and inhibits Chikungunya virus

Wolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and certain strains have also been shown to inhibit the replication or dissemination of viruses. Wolbachia strains also vary in their relative fitness effects on...

Full description

Saved in:
Bibliographic Details
Published inPLoS neglected tropical diseases Vol. 7; no. 3; p. e2152
Main Authors Blagrove, Marcus S C, Arias-Goeta, Camilo, Di Genua, Cristina, Failloux, Anna-Bella, Sinkins, Steven P
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 01.03.2013
Public Library of Science (PLoS)
Subjects
Aedes
Aedes - microbiology
Aedes - physiology
Aedes - virology
Animals
Chikungunya fever
Chikungunya virus
Chikungunya virus - growth & development
Dengue fever
Development and progression
Drosophila melanogaster
Drosophila melanogaster - microbiology
Experiments
Female
Health aspects
Host-parasite relationships
Insects
Life Sciences
Male
Males
Mathematical models
Medicine
Microbial Interactions
Microbiology and Parasitology
Mosquitoes
Mutation
Physiological aspects
Saliva
Saliva - virology
Virology
Viruses
Wolbachia
Wolbachia - growth & development
Wolbachia - isolation & purification
France
Mosquitoes
Wolbachia
Chikungunya virus
Chikungunya infection
Dengue virus
Fecundity
Saliva
Blood
Animals
Female
Male
Aedes
Drosophila melanogaster
Microbial Interactions
Online AccessGet full text

Cover

Abstract Wolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and certain strains have also been shown to inhibit the replication or dissemination of viruses. Wolbachia strains also vary in their relative fitness effects on their hosts and this is a particularly important consideration with respect to the potential of newly created transinfections for use in disease control. In Aedes albopictus mosquitoes transinfected with the wMel strain from Drosophila melanogaster, which we previously reported to be unable to transmit dengue in lab challenges, no significant detrimental effects were observed on egg hatch rate, fecundity, adult longevity or male mating competitiveness. All these parameters influence the population dynamics of Wolbachia, and the data presented are favourable with respect to the aim of taking wMel to high population frequency. Challenge with the chikungunya (CHIKV) virus, for which Ae. albopictus is an important vector, was conducted and the presence of wMel abolished CHIKV dissemination to the saliva. Taken together, these data suggest that introducing wMel into natural Ae. albopictus populations using bidirectional CI could be an efficient strategy for preventing or reducing the transmission of arboviruses by this species.
AbstractList The tiger mosquito Aedes albopictus is an invasive disease vector whose range has expanded throughout the tropics, and some temperate regions, in recent decades from its native South East Asia. It is an important vector of human viruses including dengue and chikungunya; in recent years a mutation has been detected in chikungunya virus that specifically increases transmission efficiency by Ae. albopictus , causing concern that epidemics of this disease will become more widespread and severe. Here we show that when transinfected with a strain of the symbiont Wolbachia called w Mel, originating in fruitflies, the ability of the mosquito to transmit the mutated chikungunya virus was abolished in lab experiments. Furthermore, the w Mel strain was shown to produce no detectable fitness costs in this new host, examining numbers of eggs produced, egg hatch, lifespan, and male mating competitiveness compared to wildtypes. This is encouraging with respect to developing the system for use in the control of dengue and chikungunya viruses.
BACKGROUNDWolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and certain strains have also been shown to inhibit the replication or dissemination of viruses. Wolbachia strains also vary in their relative fitness effects on their hosts and this is a particularly important consideration with respect to the potential of newly created transinfections for use in disease control. METHODOLOGY/PRINCIPAL FINDINGSIn Aedes albopictus mosquitoes transinfected with the wMel strain from Drosophila melanogaster, which we previously reported to be unable to transmit dengue in lab challenges, no significant detrimental effects were observed on egg hatch rate, fecundity, adult longevity or male mating competitiveness. All these parameters influence the population dynamics of Wolbachia, and the data presented are favourable with respect to the aim of taking wMel to high population frequency. Challenge with the chikungunya (CHIKV) virus, for which Ae. albopictus is an important vector, was conducted and the presence of wMel abolished CHIKV dissemination to the saliva. CONCLUSIONS/SIGNIFICANCETaken together, these data suggest that introducing wMel into natural Ae. albopictus populations using bidirectional CI could be an efficient strategy for preventing or reducing the transmission of arboviruses by this species.
  Background Wolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and certain strains have also been shown to inhibit the replication or dissemination of viruses. Wolbachia strains also vary in their relative fitness effects on their hosts and this is a particularly important consideration with respect to the potential of newly created transinfections for use in disease control. Methodology/Principal Findings In Aedes albopictus mosquitoes transinfected with the wMel strain from Drosophila melanogaster, which we previously reported to be unable to transmit dengue in lab challenges, no significant detrimental effects were observed on egg hatch rate, fecundity, adult longevity or male mating competitiveness. All these parameters influence the population dynamics of Wolbachia, and the data presented are favourable with respect to the aim of taking wMel to high population frequency. Challenge with the chikungunya (CHIKV) virus, for which Ae. albopictus is an important vector, was conducted and the presence of wMel abolished CHIKV dissemination to the saliva. Conclusions/significance Taken together, these data suggest that introducing wMel into natural Ae. albopictus populations using bidirectional CI could be an efficient strategy for preventing or reducing the transmission of arboviruses by this species.
Wolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and certain strains have also been shown to inhibit the replication or dissemination of viruses. Wolbachia strains also vary in their relative fitness effects on their hosts and this is a particularly important consideration with respect to the potential of newly created transinfections for use in disease control. In Aedes albopictus mosquitoes transinfected with the wMel strain from Drosophila melanogaster, which we previously reported to be unable to transmit dengue in lab challenges, no significant detrimental effects were observed on egg hatch rate, fecundity, adult longevity or male mating competitiveness. All these parameters influence the population dynamics of Wolbachia, and the data presented are favourable with respect to the aim of taking wMel to high population frequency. Challenge with the chikungunya (CHIKV) virus, for which Ae. albopictus is an important vector, was conducted and the presence of wMel abolished CHIKV dissemination to the saliva. Taken together, these data suggest that introducing wMel into natural Ae. albopictus populations using bidirectional CI could be an efficient strategy for preventing or reducing the transmission of arboviruses by this species.
Background: Wolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and certain strains have also been shown to inhibit the replication or dissemination of viruses. Wolbachia strains also vary in their relative fitness effects on their hosts and this is a particularly important consideration with respect to the potential of newly created transinfections for use in disease control. Methodology/Principal Findings: In Aedes albopictus mosquitoes transinfected with the wMel strain from Drosophila melanogaster, which we previously reported to be unable to transmit dengue in lab challenges, no significant detrimental effects were observed on egg hatch rate, fecundity, adult longevity or male mating competitiveness. All these parameters influence the population dynamics of Wolbachia, and the data presented are favourable with respect to the aim of taking wMel to high population frequency. Challenge with the chikungunya (CHIKV) virus, for which Ae. albopictus is an important vector, was conducted and the presence of wMel abolished CHIKV dissemination to the saliva. Conclusions/significance: Taken together, these data suggest that introducing wMel into natural Ae. albopictus populations using bidirectional CI could be an efficient strategy for preventing or reducing the transmission of arboviruses by this species.
Background: Wolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and certain strains have also been shown to inhibit the replication or dissemination of viruses. Wolbachia strains also vary in their relative fitness effects on their hosts and this is a particularly important consideration with respect to the potential of newly created transinfections for use in disease control.Methodology/Principal FindingsIn Aedes albopictus mosquitoes transinfected with the wMel strain from Drosophila melanogaster, which we previously reported to be unable to transmit dengue in lab challenges, no significant detrimental effects were observed on egg hatch rate, fecundity, adult longevity or male mating competitiveness. All these parameters influence the population dynamics of Wolbachia, and the data presented are favourable with respect to the aim of taking wMel to high population frequency. Challenge with the chikungunya (CHIKV) virus, for which Ae. albopictus is an important vector, was conducted and the presence of wMel abolished CHIKV dissemination to the saliva.Conclusions/significanceTaken together, these data suggest that introducing wMel into natural Ae. albopictus populations using bidirectional CI could be an efficient strategy for preventing or reducing the transmission of arboviruses by this species.
Wolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and certain strains have also been shown to inhibit the replication or dissemination of viruses. Wolbachia strains also vary in their relative fitness effects on their hosts and this is a particularly important consideration with respect to the potential of newly created transinfections for use in disease control.In Aedes albopictus mosquitoes transinfected with the wMel strain from Drosophila melanogaster, which we previously reported to be unable to transmit dengue in lab challenges, no significant detrimental effects were observed on egg hatch rate, fecundity, adult longevity or male mating competitiveness. All these parameters influence the population dynamics of Wolbachia, and the data presented are favourable with respect to the aim of taking wMel to high population frequency. Challenge with the chikungunya (CHIKV) virus, for which Ae. albopictus is an important vector, was conducted and the presence of wMel abolished CHIKV dissemination to the saliva.Taken together, these data suggest that introducing wMel into natural Ae. albopictus populations using bidirectional CI could be an efficient strategy for preventing or reducing the transmission of arboviruses by this species.
Audience Academic
Author Failloux, Anna-Bella
Sinkins, Steven P
Blagrove, Marcus S C
Di Genua, Cristina
Arias-Goeta, Camilo
AuthorAffiliation 2 Institut Pasteur, Department of Virology, Paris, France
Monash University, Australia
1 University of Oxford, Peter Medawar Building for Pathogen Research and Departments of Medicine (NDM)/Zoology, Oxford, United Kingdom
AuthorAffiliation_xml – name: 1 University of Oxford, Peter Medawar Building for Pathogen Research and Departments of Medicine (NDM)/Zoology, Oxford, United Kingdom
– name: 2 Institut Pasteur, Department of Virology, Paris, France
– name: Monash University, Australia
Author_xml – sequence: 1
  givenname: Marcus S C
  surname: Blagrove
  fullname: Blagrove, Marcus S C
  organization: University of Oxford, Peter Medawar Building for Pathogen Research and Departments of Medicine (NDM)/Zoology, Oxford, United Kingdom
– sequence: 2
  givenname: Camilo
  surname: Arias-Goeta
  fullname: Arias-Goeta, Camilo
– sequence: 3
  givenname: Cristina
  surname: Di Genua
  fullname: Di Genua, Cristina
– sequence: 4
  givenname: Anna-Bella
  surname: Failloux
  fullname: Failloux, Anna-Bella
– sequence: 5
  givenname: Steven P
  surname: Sinkins
  fullname: Sinkins, Steven P
BackLink https://www.ncbi.nlm.nih.gov/pubmed/23556030$$D View this record in MEDLINE/PubMed
https://pasteur.hal.science/pasteur-01680359$$DView record in HAL
BookMark eNptkl2PEyEYhSdmjfuh_8AoiYnxphWGgRluNmkadTep8UbjJWH46FApVGDW7L-X2XY3rTFcQOA5B96Xc1md-eB1Vb1GcI5wiz5uwhi9cPOdz2oOIawRqZ9VF4hhMqtbTM6O1ufVZUobCAkjHXpRndeYEAoxvKjGBfgZXC_kYAX481U7kKPwyXqjZbbBA-vBQiudgHB92FmZxwRsAj5koHSOdqt9FkUVwBBSBsZmr1OhvSrSwfY2J7Ac7K_Rr0d_L8CdjWN6WT03wiX96jBfVT8-f_q-vJmtvn25XS5WM0lxk2eY1NBoSGWrW6oM1S1qEFREQoVMB1HHqFSQEWJMgwVGEhHRMUgIYxBj2OKr6u3ed-dC4oeOJY5w3dYNa9qJuN0TKogN35V6RLznQVj-sBHimouYrXSa657IjtaNqQ1rlCGsU6pHrZCS9Ew0sHhdH24b-61WsnQmCndienri7cDX4Y5jiiBt6mIw2xsM_8huFiu-EynrMXKIaAcxYXeo8B8OF8bwe9Qp861NUjsnvA7jQ50N7ihlXUHf7dG1KKWU7w3lBXLC-QLXpduMoclw_h-qDKW3Vpb8GVv2TwTvjwSDFi4PKbhxik46BZs9KGNIKWrzVB-CfMrz4-_wKc_8kOcie3Pc0ifRY4DxX4GU9GQ
CitedBy_id crossref_primary_10_1371_journal_pone_0094094
crossref_primary_10_1128_mBio_03472_20
crossref_primary_10_1038_srep03932
crossref_primary_10_1128_mBio_00738_18
crossref_primary_10_1186_s13071_020_04466_8
crossref_primary_10_7717_peerj_4905
crossref_primary_10_1099_vir_0_057422_0
crossref_primary_10_1002_14651858_CD015636_pub2
crossref_primary_10_1186_1756_3305_7_336
crossref_primary_10_1016_j_antiviral_2015_10_015
crossref_primary_10_1016_j_onehlt_2016_02_001
crossref_primary_10_3389_fmicb_2020_01638
crossref_primary_10_1186_1471_2164_15_928
crossref_primary_10_1016_j_actatropica_2013_11_004
crossref_primary_10_1111_1748_5967_12430
crossref_primary_10_3390_v6114628
crossref_primary_10_1371_journal_pntd_0005158
crossref_primary_10_1371_journal_pntd_0003576
crossref_primary_10_1111_brv_12098
crossref_primary_10_3390_v10040141
crossref_primary_10_7883_yoken_JJID_2021_376
crossref_primary_10_1128_AEM_02841_15
crossref_primary_10_1371_journal_ppat_1007445
crossref_primary_10_1093_procel_pwad021
crossref_primary_10_1016_j_virol_2018_12_006
crossref_primary_10_1002_14651858_CD015636
crossref_primary_10_1002_ece3_3676
crossref_primary_10_1038_s41579_018_0025_0
crossref_primary_10_1007_s13199_022_00836_1
crossref_primary_10_1371_journal_pntd_0007926
crossref_primary_10_3389_fmicb_2019_00022
crossref_primary_10_1089_vbz_2014_1745
crossref_primary_10_1016_j_pt_2015_10_011
crossref_primary_10_1146_annurev_virology_031413_085513
crossref_primary_10_3390_v7082836
crossref_primary_10_1038_s42003_023_05180_8
crossref_primary_10_1111_eva_12286
crossref_primary_10_1016_j_pt_2013_07_003
crossref_primary_10_1016_j_cub_2019_11_007
crossref_primary_10_1093_infdis_jiw397
crossref_primary_10_1128_JVI_00339_17
crossref_primary_10_2217_fmb_13_95
crossref_primary_10_12688_wellcomeopenres_12468_1
crossref_primary_10_1371_journal_pone_0121813
crossref_primary_10_3389_fmicb_2021_624170
crossref_primary_10_1186_s13071_018_2870_0
crossref_primary_10_1111_aen_12068
crossref_primary_10_1371_journal_pone_0151864
crossref_primary_10_1371_journal_ppat_1005536
crossref_primary_10_1016_j_pt_2013_06_005
crossref_primary_10_1038_s41598_018_34640_z
crossref_primary_10_1038_s41598_019_48414_8
crossref_primary_10_3390_v15020469
crossref_primary_10_1089_dna_2017_4025
crossref_primary_10_1007_s40475_016_0066_2
crossref_primary_10_1186_s13071_021_04742_1
crossref_primary_10_3390_pathogens9050404
crossref_primary_10_1038_s41467_017_00610_8
crossref_primary_10_1371_journal_pone_0125066
crossref_primary_10_1128_AEM_00275_16
crossref_primary_10_1371_journal_pntd_0003115
crossref_primary_10_3390_insects6010236
crossref_primary_10_1371_journal_pntd_0006666
crossref_primary_10_1186_s13071_021_04937_6
crossref_primary_10_1093_jme_tjw096
crossref_primary_10_3389_fcimb_2014_00029
crossref_primary_10_1134_S2079086422040065
crossref_primary_10_1016_j_actatropica_2022_106539
crossref_primary_10_1016_j_actatropica_2016_01_009
crossref_primary_10_1038_srep39200
crossref_primary_10_1186_s13071_015_1174_x
crossref_primary_10_1242_jeb_140699
crossref_primary_10_1371_journal_ppat_1006815
crossref_primary_10_1016_j_coviro_2016_09_014
crossref_primary_10_1016_j_actatropica_2021_106045
crossref_primary_10_1016_j_cois_2019_02_005
crossref_primary_10_1080_20477724_2022_2117939
crossref_primary_10_3390_v7112903
crossref_primary_10_1038_s41598_023_38127_4
crossref_primary_10_1002_rmv_1941
crossref_primary_10_1371_journal_pntd_0004677
crossref_primary_10_3389_fmicb_2023_1138476
Cites_doi 10.1371/journal.ppat.1002412
10.1111/j.0269-283X.2004.00513.x
10.1051/parasite/2008151003
10.1371/journal.ppat.0030201
10.1038/nrmicro1326
10.1098/rspb.1995.0154
10.1093/genetics/160.3.1087
10.1073/pnas.1112021108
10.1126/science.1165326
10.1371/journal.pone.0005895
10.1126/science.8511587
10.1038/nrmicro2368
10.1016/j.cell.2009.11.042
10.1038/sj.hdy.6800458
10.1128/JB.185.24.7077-7084.2003
10.1128/AEM.01331-09
10.1371/journal.pntd.0001892
10.3201/eid1705.101873
10.1126/science.1201618
10.1038/nature10355
10.1126/science.1177531
10.1073/pnas.052466499
10.1016/S0140-6736(11)60281-X
10.1089/vbz.2011.0736
10.1371/journal.pone.0001168
10.1038/nature10356
10.1038/nbt.2019
10.1590/S0074-02762011000800003
10.1086/658121
10.1371/journal.pmed.0030263
10.4269/ajtmh.2002.66.108
10.1073/pnas.1116932108
10.1016/j.jviromet.2006.01.003
10.1111/j.1365-294X.2010.04606.x
10.1073/pnas.94.20.10792
10.1371/journal.pntd.0001024
10.1016/S0140-6736(07)61779-6
10.1126/science.1117607
10.1016/j.coviro.2011.07.004
10.1073/pnas.1018344108
ContentType Journal Article
Copyright COPYRIGHT 2013 Public Library of Science
Attribution
2013 Blagrove et al 2013 Blagrove et al
2013 Blagrove et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Blagrove MSC, Arias-Goeta C, Di Genua C, Failloux A-B, Sinkins SP (2013) A Wolbachia wMel Transinfection in Aedes albopictus Is Not Detrimental to Host Fitness and Inhibits Chikungunya Virus. PLoS Negl Trop Dis 7(3): e2152. doi:10.1371/journal.pntd.0002152
Copyright_xml – notice: COPYRIGHT 2013 Public Library of Science
– notice: Attribution
– notice: 2013 Blagrove et al 2013 Blagrove et al
– notice: 2013 Blagrove et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Blagrove MSC, Arias-Goeta C, Di Genua C, Failloux A-B, Sinkins SP (2013) A Wolbachia wMel Transinfection in Aedes albopictus Is Not Detrimental to Host Fitness and Inhibits Chikungunya Virus. PLoS Negl Trop Dis 7(3): e2152. doi:10.1371/journal.pntd.0002152
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
1XC
VOOES
5PM
DOA
DOI 10.1371/journal.pntd.0002152
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
Hyper Article en Ligne (HAL)
Hyper Article en Ligne (HAL) (Open Access)
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList
MEDLINE - Academic

MEDLINE
Database_xml – sequence: 1
  dbid: DOA
  name: Open Access: DOAJ - Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
DocumentTitleAlternate Wolbachia wMel in Aedes albopictus: Fitness & CHIKV
EISSN 1935-2735
Editor O'Neill, Scott L.
Editor_xml – sequence: 1
  givenname: Scott L.
  surname: O'Neill
  fullname: O'Neill, Scott L.
EndPage e2152
ExternalDocumentID 1327249477
oai_doaj_org_article_eb5c8624f2f94df598ddb17acc5b9a40
oai_HAL_pasteur_01680359v1
A326349911
10_1371_journal_pntd_0002152
23556030
Genre Research Support, Non-U.S. Gov't
Journal Article
GeographicLocations France
GeographicLocations_xml – name: France
GrantInformation_xml – fundername: Wellcome Trust
  grantid: 095121
– fundername: Wellcome Trust
– fundername: Biotechnology and Biological Sciences Research Council
  grantid: BB/G016976/1
GroupedDBID ---
123
29O
2WC
3V.
53G
5VS
7X7
88E
8C1
8FI
8FJ
AAFWJ
ABDBF
ABUWG
ACGFO
ACIHN
ACPRK
ADBBV
ADRAZ
AEAQA
AENEX
AFKRA
AFRAH
AHMBA
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BCNDV
BENPR
BPHCQ
BVXVI
BWKFM
CCPQU
CGR
CS3
CUY
CVF
DIK
DU5
E3Z
EAP
EAS
EBD
ECGQY
ECM
EIF
EMOBN
ESX
F5P
FPL
FYUFA
GROUPED_DOAJ
GX1
H13
HMCUK
HYE
IAO
IHR
IHW
IPNFZ
ITC
KQ8
M1P
M48
M~E
NPM
O5R
O5S
OK1
P2P
PGMZT
PIMPY
PQQKQ
PROAC
PSQYO
PV9
RIG
RNS
RPM
RZL
SV3
TR2
TUS
UKHRP
WOQ
AAYXX
AFPKN
CITATION
7X8
1XC
VOOES
5PM
AAPBV
ABPTK
N95
PQEST
PQUKI
ID FETCH-LOGICAL-c634t-3520fe06c7e76df6e71410d5c0d1f801896cd0955ff43a31c15a8905599033073
IEDL.DBID RPM
ISSN 1935-2735
1935-2727
IngestDate Sun May 07 16:29:03 EDT 2023
Tue Oct 22 15:15:09 EDT 2024
Tue Sep 17 21:26:07 EDT 2024
Tue Oct 15 15:39:18 EDT 2024
Fri Oct 25 06:30:20 EDT 2024
Thu Feb 22 23:31:24 EST 2024
Fri Feb 02 04:21:34 EST 2024
Tue Aug 20 22:14:55 EDT 2024
Thu Sep 12 20:01:52 EDT 2024
Wed Oct 16 00:45:44 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 3
Keywords Mosquitoes
Wolbachia
Chikungunya virus
Chikungunya infection
Dengue virus
Fecundity
Saliva
Blood
Animals
Female
Male
Aedes
Drosophila melanogaster
Microbial Interactions
Language English
License Attribution: http://creativecommons.org/licenses/by
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
Creative Commons Attribution License
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c634t-3520fe06c7e76df6e71410d5c0d1f801896cd0955ff43a31c15a8905599033073
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMCID: PMC3610642
Conceived and designed the experiments: MSCB SPS ABF. Performed the experiments: MSCB CAG CDG. Analyzed the data: MSCB ABF SPS. Wrote the paper: MSCB SPS.
The authors have declared that no competing interests exist.
ORCID 0000-0001-6890-0820
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610642/
PMID 23556030
PQID 1324386698
PQPubID 23479
ParticipantIDs plos_journals_1327249477
doaj_primary_oai_doaj_org_article_eb5c8624f2f94df598ddb17acc5b9a40
pubmedcentral_primary_oai_pubmedcentral_nih_gov_3610642
hal_primary_oai_HAL_pasteur_01680359v1
proquest_miscellaneous_1324386698
gale_infotracmisc_A326349911
gale_infotracacademiconefile_A326349911
gale_healthsolutions_A326349911
crossref_primary_10_1371_journal_pntd_0002152
pubmed_primary_23556030
PublicationCentury 2000
PublicationDate 2013-03-01
PublicationDateYYYYMMDD 2013-03-01
PublicationDate_xml – month: 03
  year: 2013
  text: 2013-03-01
  day: 01
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: San Francisco, USA
PublicationTitle PLoS neglected tropical diseases
PublicationTitleAlternate PLoS Negl Trop Dis
PublicationYear 2013
Publisher Public Library of Science
Public Library of Science (PLoS)
Publisher_xml – name: Public Library of Science
– name: Public Library of Science (PLoS)
References Z Xi (ref33) 2005; 310
SL Dobson (ref10) 2004; 93
SP Sinkins (ref2) 1995; 261
H Delatte (ref18) 2008; 15
FJ Burt (ref16) 2012; 379
M Dubrulle (ref30) 2009; 4
EA McGraw (ref32) 2002; 99
NG Gratz (ref1) 2004; 18
M Vazeille (ref23) 2007; 2
AA Hoffmann (ref41) 2011; 476
MSC Blagrove (ref4) 2012; 109
SL Dobson (ref9) 2002; 160
M Calvitti (ref35) 2010; 47
AF van den Hurk (ref37) 2012; 6
G Rezza (ref19) 2007; 370
KA Tsetsarkin (ref26) 2011; 7
KA Tsetsarkin (ref25) 2007; 3
LA Moreira (ref7) 2009; 139
KT Min (ref11) 1997; 94
PA Hancock (ref14) 2011; 177
PA Hancock (ref15) 2011; 5
M Kielian (ref29) 2006; 4
Z Kambris (ref8) 2009; 326
E Suh (ref13) 2009; 75
I Schuffenecker (ref24) 2006; 3
KA Tsetsarkin (ref28) 2011; 108
L Boyle (ref34) 1993; 260
M Grandadam (ref20) 2011; 17
CM Cirimotich (ref39) 2011; 322
AF Payne (ref31) 2006; 134
L Mousson (ref36) 2010; 19
T Walker (ref6) 2011; 476
X Pan (ref38) 2012; 109
NP Kumar (ref21) 2011; 106
LV Sun (ref5) 2003; 185
C Paupy (ref22) 2012; 12
KA Tsetsarkin (ref27) 2011; 1
P Kittayapong (ref3) 2002; 66
AF Harris (ref40) 2011; 29
O Schwartz (ref17) 2010; 8
CJ McMeniman (ref12) 2009; 323
References_xml – volume: 7
  start-page: e1002412
  year: 2011
  ident: ref26
  article-title: Sequential adaptive mutations enhance efficient vector switching by Chikungunya virus and its epidemic emergence
  publication-title: PLoS Pathog
  doi: 10.1371/journal.ppat.1002412
  contributor:
    fullname: KA Tsetsarkin
– volume: 18
  start-page: 215
  year: 2004
  ident: ref1
  article-title: Critical review of the vector status of Aedes albopictus
  publication-title: Med Vet Entomol
  doi: 10.1111/j.0269-283X.2004.00513.x
  contributor:
    fullname: NG Gratz
– volume: 15
  start-page: 3
  year: 2008
  ident: ref18
  article-title: [Aedes albopictus, vector of chikungunya and dengue viruses in Reunion Island: biology and control]
  publication-title: Parasite
  doi: 10.1051/parasite/2008151003
  contributor:
    fullname: H Delatte
– volume: 3
  start-page: e201
  year: 2007
  ident: ref25
  article-title: A single mutation in chikungunya virus affects vector specificity and epidemic potential
  publication-title: PLoS Pathog
  doi: 10.1371/journal.ppat.0030201
  contributor:
    fullname: KA Tsetsarkin
– volume: 4
  start-page: 67
  year: 2006
  ident: ref29
  article-title: Virus membrane-fusion proteins: more than one way to make a hairpin
  publication-title: Nat Rev Microbiol
  doi: 10.1038/nrmicro1326
  contributor:
    fullname: M Kielian
– volume: 261
  start-page: 325
  year: 1995
  ident: ref2
  article-title: Wolbachia superinfections and the expression of cytoplasmic incompatibility
  publication-title: Proc Biol Sci
  doi: 10.1098/rspb.1995.0154
  contributor:
    fullname: SP Sinkins
– volume: 160
  start-page: 1087
  year: 2002
  ident: ref9
  article-title: Mutualistic Wolbachia infection in Aedes albopictus: accelerating cytoplasmic drive
  publication-title: Genetics
  doi: 10.1093/genetics/160.3.1087
  contributor:
    fullname: SL Dobson
– volume: 109
  start-page: 255
  year: 2012
  ident: ref4
  article-title: The Wolbachia strain wMel induces cytoplasmic incompatibility in and blocks dengue transmission by Aedes albopictus
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.1112021108
  contributor:
    fullname: MSC Blagrove
– volume: 323
  start-page: 141
  year: 2009
  ident: ref12
  article-title: Stable introduction of a life-shortening Wolbachia infection into the mosquito Aedes aegypti
  publication-title: Science
  doi: 10.1126/science.1165326
  contributor:
    fullname: CJ McMeniman
– volume: 4
  start-page: e5895
  year: 2009
  ident: ref30
  article-title: Chikungunya virus and Aedes mosquitoes: saliva is infectious as soon as two days after oral infection
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0005895
  contributor:
    fullname: M Dubrulle
– volume: 260
  start-page: 1796
  year: 1993
  ident: ref34
  article-title: Interspecific and intraspecific horizontal transfer of Wolbachia in Drosophila
  publication-title: Science
  doi: 10.1126/science.8511587
  contributor:
    fullname: L Boyle
– volume: 8
  start-page: 491
  year: 2010
  ident: ref17
  article-title: Biology and pathogenesis of chikungunya virus
  publication-title: Nat Rev Microbiol
  doi: 10.1038/nrmicro2368
  contributor:
    fullname: O Schwartz
– volume: 139
  start-page: 1268
  year: 2009
  ident: ref7
  article-title: A Wolbachia symbiont in Aedes aegypti limits infection with dengue, Chikungunya, and Plasmodium
  publication-title: Cell
  doi: 10.1016/j.cell.2009.11.042
  contributor:
    fullname: LA Moreira
– volume: 93
  start-page: 135
  year: 2004
  ident: ref10
  article-title: Fitness advantage and cytoplasmic incompatibility in Wolbachia single- and superinfected Aedes albopictus
  publication-title: Heredity
  doi: 10.1038/sj.hdy.6800458
  contributor:
    fullname: SL Dobson
– volume: 185
  start-page: 7077
  year: 2003
  ident: ref5
  article-title: Development of a physical and genetic map of the virulent Wolbachia strain wMelPop
  publication-title: J Bacteriol
  doi: 10.1128/JB.185.24.7077-7084.2003
  contributor:
    fullname: LV Sun
– volume: 75
  start-page: 7783
  year: 2009
  ident: ref13
  article-title: Pathogenicity of life-shortening Wolbachia in Aedes albopictus after transfer from Drosophila melanogaster
  publication-title: Appl Environ Microbiol
  doi: 10.1128/AEM.01331-09
  contributor:
    fullname: E Suh
– volume: 6
  start-page: e1892
  year: 2012
  ident: ref37
  article-title: Impact of Wolbachia on infection with chikungunya and yellow fever viruses in the mosquito vector Aedes aegypti
  publication-title: PLoS Negl Trop Dis
  doi: 10.1371/journal.pntd.0001892
  contributor:
    fullname: AF van den Hurk
– volume: 17
  start-page: 910
  year: 2011
  ident: ref20
  article-title: Chikungunya virus, southeastern france
  publication-title: Emerg Infect Dis
  doi: 10.3201/eid1705.101873
  contributor:
    fullname: M Grandadam
– volume: 322
  start-page: 855
  year: 2011
  ident: ref39
  article-title: Natural Microbe-Mediated Refractoriness to Plasmodium Infection in Anopheles gambiae
  publication-title: Science
  doi: 10.1126/science.1201618
  contributor:
    fullname: CM Cirimotich
– volume: 47
  start-page: 179
  year: 2010
  ident: ref35
  article-title: Characterization of a new Aedes albopictus (Diptera: Culicidae)-Wolbachia pipientis (Rickettsiales: Rickettsiaceae) symbiotic association generated by artificial transfer of the wPip strain from Culex pipiens (Diptera: Culicidae)
  publication-title: J Med Entomol
  contributor:
    fullname: M Calvitti
– volume: 476
  start-page: 450
  year: 2011
  ident: ref6
  article-title: The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations
  publication-title: Nature
  doi: 10.1038/nature10355
  contributor:
    fullname: T Walker
– volume: 326
  start-page: 134
  year: 2009
  ident: ref8
  article-title: Immune activation by life- shortening Wolbachia and reduced filarial competence in mosquitoes
  publication-title: Science
  doi: 10.1126/science.1177531
  contributor:
    fullname: Z Kambris
– volume: 99
  start-page: 2918
  year: 2002
  ident: ref32
  article-title: Wolbachia density and virulence attenuation after transfer into a novel host
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.052466499
  contributor:
    fullname: EA McGraw
– volume: 379
  start-page: 662
  year: 2012
  ident: ref16
  article-title: Chikungunya: a re-emerging virus
  publication-title: Lancet
  doi: 10.1016/S0140-6736(11)60281-X
  contributor:
    fullname: FJ Burt
– volume: 12
  start-page: 167
  year: 2012
  ident: ref22
  article-title: A chikungunya outbreak associated with the vector Aedes albopictus in remote villages of Gabon
  publication-title: Vector Borne Zoonotic Dis
  doi: 10.1089/vbz.2011.0736
  contributor:
    fullname: C Paupy
– volume: 2
  start-page: e1168
  year: 2007
  ident: ref23
  article-title: Two Chikungunya Isolates from the Outbreak of La Reunion (Indian Ocean) Exhibit Different Patterns of Infection in the Mosquito, Aedes albopictus
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0001168
  contributor:
    fullname: M Vazeille
– volume: 476
  start-page: 454
  year: 2011
  ident: ref41
  article-title: Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission
  publication-title: Nature
  doi: 10.1038/nature10356
  contributor:
    fullname: AA Hoffmann
– volume: 29
  start-page: 1034
  year: 2011
  ident: ref40
  article-title: Field performance of engineered male mosquitoes
  publication-title: Nat Biotechnol
  doi: 10.1038/nbt.2019
  contributor:
    fullname: AF Harris
– volume: 106
  start-page: 912
  year: 2011
  ident: ref21
  article-title: Chikungunya virus outbreak in Kerala, India, 2007: a seroprevalence study
  publication-title: Mem Inst Oswaldo Cruz
  doi: 10.1590/S0074-02762011000800003
  contributor:
    fullname: NP Kumar
– volume: 177
  start-page: 323
  year: 2011
  ident: ref14
  article-title: Population dynamic models of the spread of Wolbachia
  publication-title: Am Nat
  doi: 10.1086/658121
  contributor:
    fullname: PA Hancock
– volume: 3
  start-page: e263
  year: 2006
  ident: ref24
  article-title: Genome microevolution of chikungunya viruses causing the Indian Ocean outbreak
  publication-title: PLoS Med
  doi: 10.1371/journal.pmed.0030263
  contributor:
    fullname: I Schuffenecker
– volume: 66
  start-page: 108
  year: 2002
  ident: ref3
  article-title: Field prevalence of Wolbachia in the mosquito vector Aedes albopictus
  publication-title: Am J Trop Med Hyg
  doi: 10.4269/ajtmh.2002.66.108
  contributor:
    fullname: P Kittayapong
– volume: 109
  start-page: E23
  year: 2012
  ident: ref38
  article-title: Wolbachia induces reactive oxygen species (ROS)-dependent activation of the Toll pathway to control dengue virus in the mosquito Aedes aegypti
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.1116932108
  contributor:
    fullname: X Pan
– volume: 134
  start-page: 183
  year: 2006
  ident: ref31
  article-title: Quantitation of flaviviruses by fluorescent focus assay
  publication-title: J Virol Methods
  doi: 10.1016/j.jviromet.2006.01.003
  contributor:
    fullname: AF Payne
– volume: 19
  start-page: 1953
  year: 2010
  ident: ref36
  article-title: Wolbachia modulates Chikungunya replication in Aedes albopictus
  publication-title: Mol Ecol
  doi: 10.1111/j.1365-294X.2010.04606.x
  contributor:
    fullname: L Mousson
– volume: 94
  start-page: 10792
  year: 1997
  ident: ref11
  article-title: Wolbachia, normally a symbiont of Drosophila, can be virulent, causing degeneration and early death
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.94.20.10792
  contributor:
    fullname: KT Min
– volume: 5
  start-page: e1024
  year: 2011
  ident: ref15
  article-title: Strategies for introducing Wolbachia to reduce transmission of mosquito-borne diseases
  publication-title: PLoS Negl Trop Dis
  doi: 10.1371/journal.pntd.0001024
  contributor:
    fullname: PA Hancock
– volume: 370
  start-page: 1840
  year: 2007
  ident: ref19
  article-title: Infection with chikungunya virus in Italy: an outbreak in a temperate region
  publication-title: Lancet
  doi: 10.1016/S0140-6736(07)61779-6
  contributor:
    fullname: G Rezza
– volume: 310
  start-page: 326
  year: 2005
  ident: ref33
  article-title: Wolbachia establishment and invasion in an Aedes aegypti laboratory population
  publication-title: Science
  doi: 10.1126/science.1117607
  contributor:
    fullname: Z Xi
– volume: 1
  start-page: 310
  year: 2011
  ident: ref27
  article-title: Chikungunya Virus: Evolution and Genetic Determinants of Emergence
  publication-title: Curr Opin Virol
  doi: 10.1016/j.coviro.2011.07.004
  contributor:
    fullname: KA Tsetsarkin
– volume: 108
  start-page: 7872
  year: 2011
  ident: ref28
  article-title: Chikungunya virus emergence is constrained in Asia by lineage-specific adaptive landscapes
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.1018344108
  contributor:
    fullname: KA Tsetsarkin
SSID ssj0059581
Score 2.4160798
Snippet Wolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and certain strains...
Background: Wolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and...
BACKGROUNDWolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and...
The tiger mosquito Aedes albopictus is an invasive disease vector whose range has expanded throughout the tropics, and some temperate regions, in recent...
  Background Wolbachia inherited intracellular bacteria can manipulate the reproduction of their insect hosts through cytoplasmic incompatibility (CI), and...
SourceID plos
doaj
pubmedcentral
hal
proquest
gale
crossref
pubmed
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
StartPage e2152
SubjectTerms Aedes
Aedes - microbiology
Aedes - physiology
Aedes - virology
Animals
Chikungunya fever
Chikungunya virus
Chikungunya virus - growth & development
Dengue fever
Development and progression
Drosophila melanogaster
Drosophila melanogaster - microbiology
Experiments
Female
Health aspects
Host-parasite relationships
Insects
Life Sciences
Male
Males
Mathematical models
Medicine
Microbial Interactions
Microbiology and Parasitology
Mosquitoes
Mutation
Physiological aspects
Saliva
Saliva - virology
Virology
Viruses
Wolbachia
Wolbachia - growth & development
Wolbachia - isolation & purification
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9NAEF5BDogL4l3TAouE4GRqx_uwj6GiihDhREVvq_U-FKvROortIv59Z2wnihESF672JPbOe-SZbwh5z7BmyDmP51ZDgVKkLC65tzEULLlJNNdC4IDz6rtYXrGv1_z6aNUX9oQN8MAD485dyQ0OMfi5L5j1vMitLVOpjeFlodlQrSfFvpgafDAveL-eFLITnLiay3FoLpPp-SijT9vQWkQvxMWuk6DUY_cfPPT9NTZIzrabuvlbEvpnL-VRcLp8TB6NWSVdDKd5Qu658JQ8WI3fzZ-RbkF_1psSGyc1_bVyG9pijNp3YgVaBbpw1jVUb8p6W5m2a2jV0FC31OLOrWEFAG1rilMh1Fctukiqg4Wfrquyaht6sa5uwHV04bemt9Wua56Tq8svPy6W8bhwITYiY20MyVjiXSKMdFJYL5zELlDLTWJTD6EsL4SxiFnnPct0lpqU67xIELQsydBZvCCzUAd3QijwQ3rnEQ4M4iTIyvrS88wwmfsUiCMS7zmutgOuhuo_rkmoRwamKZSQGiUUkc8olgMtomL3F0BX1Kgr6l-6EpG3KFQ1jJgebFstIIfNoPRL04h87CnQukEQRo9DCnAoxMmaUJ5NKMEqzeT2hzUe4eh1l4tvaqvBdrudgkQ7R_DEWyA8Qd3aH7oBFoDCsoJJGZF3e31T-PfYDhdc3fU0LMuFKPKIvBz07_CoOWSQAlx3ROREMyfvMr0TqnUPLJ5BLg316Kv_wetT8nDebw7Bdr0zMmt3nXsN-VtbvulN9Q5WdUMj
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: Scholars Portal Open Access Journals
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELbaRUJcEO-mFDASglOqPOw4OSC0VFQrxHJiRW-R4wcbsXKWTdLSf89MHqsGlRvXZPLwvDwjz3xDyBuGOUPKuR9pCQlKFjK_4Fb7kLCkKpBcJgk2OC-_JosV-3zBLw7IOLN1YGB9a2qH86RWu83p71_XH8Dg33dTG0Q4PnS6dY1GPEIc1XpI7kQIzYXFfGx_rsAz3o0thagFO7EiMTTT_estk82qw_Tfe-7DNRZOzrabqr4tOP27xvLGpnX-gNwfok0679XjITkw7hG5uxzO0x-Tdk6_V5sCCyolvVqaDW1w7xortBwtHZ0bbWoqN0W1LVXT1rSsqasaqnEWVz8agDYVxW4RassGXSeVTsOj67Iom5qercuf4FJady3pZblr6ydkdf7p29nCHwYx-CqJWeNDkBZYEyRKGJFomxiB1aGaq0CHFra4NEuURiw7a1ks41CFXKZZgGBmQYxO5CmZucqZI0KBH8IaizBhsH8qXmhbWB4rJlIbArFH_JHj-bbH28i7QzcBeUrPtBwllA8S8shHFMueFtGyuwvV7kc-GF9uCq6wEcZGNmPa8izVugiFVPD9TLLAI69QqHnferq3-XwOsW0MKWEYeuRdR4F6CIJQcmhegEUhftaE8mRCCdaqJrffrnEJN353Mf-SbyXYdLvLIQBPEVTxEgiPULfGRdfAAlBYljEhPPJ61LccX49lcs5UbUfD4jRJstQjz3r9238qgsgyAZfuETHRzMm_TO-4ct0BjscQY0Oeevw_eP2c3Iu6iSJYxndCZs2uNS8grmuKl52p_gFh_0zG
  priority: 102
  providerName: Scholars Portal
Title A Wolbachia wMel transinfection in Aedes albopictus is not detrimental to host fitness and inhibits Chikungunya virus
URI https://www.ncbi.nlm.nih.gov/pubmed/23556030
https://search.proquest.com/docview/1324386698
https://pasteur.hal.science/pasteur-01680359
https://pubmed.ncbi.nlm.nih.gov/PMC3610642
https://doaj.org/article/eb5c8624f2f94df598ddb17acc5b9a40
http://dx.doi.org/10.1371/journal.pntd.0002152
Volume 7
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Nj5NAFJ_s1sR4MX4vutYxMXqihcIwcOzWbbrGNhvjam9kmGG2xC6QAhr_e98boGmNJy8c4BWYed_l994j5J2POUPImD1RAhKUyPXthGllQ8ISSkcwEQRY4LxcBYsb_9OarU8I62thDGhfJtko396N8mxjsJXlnRz3OLHx9XLmgc-HuHl8Sk5BQPsUvTW_LGJmMikEJlhsNeFdvZzH3XHHnlGZ1wobF-JMV-wGDD43MDjoA9dkOvjv7fTpBmGSg3JbVP8KRf9GVB64qPkj8rCLLem0XcNjcpLmT8j9Zff1_ClppvR7sU0QPinor2W6pcZT9XisnGY5naYqrajYJkWZybqp6FVFV0VNP-LkrXYQAK0Luiiqms6zGg0lFbmiV_kmS7K6orNN9gMMSJP_FvRbtmuqZ-Rmfvl1trC7sQu2DDy_tiEkc3TqBJKnPFA6SDliQRWTjnI1OLQwCqTCznVa-57wXOkyEUYOti5zPDQZz8kgL_L0jFDYD65TjU3BwFtKliidaOZJn4faBWKL2P2Ox2XbXSM2n9g4ZCXtpsXIrLhjlkUukC17WuyNbU4Uu9u4k5A4TZjEshc90ZGvNItCpRKXCwnPj4TvWOQNMjVuC033Gh5PIZL1IAF0XYt8MBSo48BOKbpSBVgUdss6ojw_ogTdlEeX329wCQevu5h-jksBGtzsYgi3Q2yh-BMIz1C2-kVXsAUgu37kc26Rt728xXh7BMXladEYGt8LgyAKLfKilb_9o3qZtgg_ksyjdzm-Atpm2ot32vXyv3_5ijyYmKEhiNQ7J4N616SvIXSrkyEo7JrDMZy5Q3Lv4nJ1_WVo_gaB49IPh0aV_wAUM0ZJ
link.rule.ids 230,315,730,783,787,867,888,2109,2228,24332,27938,27939,31734,33281,33759,53806,53808
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEF61QQIuvEsNhS4SgpMTO_b6cQyBKoEk4tCW3qz1rpdYTW0rtkHw65lZ21FccYGrd_zY3dl5yN98Q8hbF3OGgDFzLDkkKKHtmjFT0oSEJRAWZ9zzsMB5ufJmF-7nK3Z1QFhXC6NB-yJOh9nmZpila42tLG7EqMOJjb4upw74fIibR4fkDpxXy-uS9MYAs5Dp3qQQmmC51dhvK-Yc3x61GzQsskoidSF2dUU-YPC6nkZC7zknzeG_s9SHawRKDopNXv4tGL2NqdxzUmcPyWU3vQabcj2sq3goft9ifvzn-T8iD9qwlU6a4cfkIMmekLvL9sf8U1JP6Ld8EyMyk9Ofy2RDtRPsoF4ZTTM6SWRSUr6J8yIVVV3SeUlXeUU_YlOvpscArXI6y8uKnqUV2mDKM0nn2TqN06qk03V6Dbapzn5xeplu6_IZuTj7dD6dmW1HB1N4jluZEO1ZKrE84Se-J5WX-AgzlUxY0lbgK4PQExJJ8ZRyHe7YwmY8CC1kRbMctEZHZJDlWXJMKCy0rxKFfGPgiAWLpYoVc4TrB8oGYYOY3VZGRUPcEem_dz4kPM2iRagFUasFBvmA-72TRdptfSHffo_axY-SmAmsqFFjFbpSsTCQMrZ9LuD9IXctg5yitkRNDevOeEQTCJIdyC1t2yDvtQSaD9ATwdsqCJgUEnH1JE96knDsRW_43RqnsPe5s8kiKjgYh3obQSQfIDvjDxA8RqXtJl3CEsChcEPX9w3yplPkCB-PeLssyWst4zqB54WBQZ43ir17VXdYDOL3VL73Lf0RUGTNXN4q7ov_vvOU3JudLxfRYr768pLcH-veJAgIPCGDalsnryBCrOLX2h78AX4WYxo
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEF7RIFVceNMaCl0kBCcnduz14xhSogSaqAcKFRdrveslVlPbim0Q_Hpm1nYUV5x6tceP3Z2dh_abbwh552LOEDBmjiWHBCW0XTNmSpqQsATC4ox7HhY4L1fe_NL9fMWu9lp9adC-iNNhtrkZZulaYyuLGzHqcGKji-XUAZ8PcfOokGp0QO7DnrWCLlFvjDALme5PCuEJllyN_bZqzvHtUbtIwyKrJNIXYmdX5AQGz-tpNPSeg9I8_jtrfbBGsOSg2OTl_wLS27jKPUc1e0R-dENs8CnXw7qKh-LvLfbHO83BY_KwDV_ppBF5Qu4l2VNyuGwP6J-RekK_55sYEZqc_l4mG6qdYQf5ymia0Ukik5LyTZwXqajqki5KusoreobNvZpeA7TK6TwvKzpLK7TFlGeSLrJ1GqdVSafr9BpsVJ394fRbuq3L5-Ry9unrdG62nR1M4TluZULUZ6nE8oSf-J5UXuIj3FQyYUlbgc8MQk9IJMdTynW4Ywub8SC0kB3NctAqvSCDLM-SY0Jhsn2VKOQdA4csWCxVrJgjXD9QNggbxOyWMyoaAo9In-L5kPg0kxahJkStJhjkI675Thbpt_WFfPszahcgSmImsLJGjVXoSsXCQMrY9rmA74fctQxyihoTNbWsOyMSTSBYdiDHtG2DfNASaEZAVwRvqyFgUEjI1ZM86UnC9he92-_XOIS9351PzqOCg5GotxFE9AGyNP4CwWNU3G7QJUwBbAw3dH3fIG87ZY7w9Yi7y5K81jKuE3heGBjkqFHu3ae6DWMQv6f2vX_p3wFl1gzmrfK-vPOTp-Tw4mwWnS9WX16RB2PdogRxgSdkUG3r5DUEilX8RpuEf4ImZZo
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+Wolbachia+wMel+transinfection+in+Aedes+albopictus+is+not+detrimental+to+host+fitness+and+inhibits+Chikungunya+virus&rft.jtitle=PLoS+neglected+tropical+diseases&rft.au=Marcus+S+C+Blagrove&rft.au=Camilo+Arias-Goeta&rft.au=Cristina+Di+Genua&rft.au=Anna-Bella+Failloux&rft.date=2013-03-01&rft.pub=Public+Library+of+Science+%28PLoS%29&rft.issn=1935-2727&rft.eissn=1935-2735&rft.volume=7&rft.issue=3&rft.spage=e2152&rft_id=info:doi/10.1371%2Fjournal.pntd.0002152&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_eb5c8624f2f94df598ddb17acc5b9a40
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1935-2735&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1935-2735&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1935-2735&client=summon