A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts

• Published in: Journal of medical virology Vol. 92; no. 8; pp. 1065 - 1074
• Main Authors: Hayes, A., Nguyen, D., Andersson, M., Antón, A., Bailly, J.‐L., Beard, S., Benschop, K. S. M., Berginc, N., Blomqvist, S., Cunningham, E., Davis, D., Dembinski, J. L., Diedrich, S., Dudman, S. G., Dyrdak, R., Eltringham, G. J. A., Gonzales‐Goggia, S., Gunson, R., Howson‐Wells, H. C., Jääskeläinen, A. J., López‐Labrador, F. X., Maier, M., Majumdar, M., Midgley, S., Mirand, A., Morley, U., Nordbø, S. A., Oikarinen, S., Osman, H., Papa, A., Pellegrinelli, L., Piralla, A., Rabella, N., Richter, J., Smith, M., Söderlund Strand, A., Templeton, K., Vipond, B., Vuorinen, T., Williams, C., Wollants, E., Zakikhany, K., Fischer, T. K., Harvala, H., Simmonds, P.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.08.2020; Wiley-Blackwell; John Wiley and Sons Inc
• Subjects: Assaying; Biodiversity and Ecology; Cerebrospinal fluid; Clinical Laboratory Medicine; Clinical Medicine; Diagnostic systems; DNA-directed RNA polymerase; enterovirus; Enterovirus - classification; enterovirus A71; Enterovirus Infections - diagnosis; Enterovirus Infections - virology; Enteroviruses; Environment and Society; Environmental Engineering; Environmental Sciences; Europe; Gene Dosage; Global Changes; Humans; Klinisk laboratoriemedicin; Klinisk medicin; Laboratories; Life Sciences; Medical and Health Sciences; Medicin och hälsovetenskap; Meningitis; Meningitis, Viral - diagnosis; Microbiology and Parasitology; Molecular Typing; parechovirus; Parechovirus - classification; PCR; Picornaviridae Infections - diagnosis; Picornaviridae Infections - virology; Polymerase chain reaction; Reagent Kits, Diagnostic; Reagents; Real-Time Polymerase Chain Reaction; Reproducibility of Results; Rhinovirus; Ribonucleic acid; RNA; RNA transcripts; RNA, Viral - genetics; Sensitivity analysis; Sensitivity and Specificity; Sepsis; Transcription; Typing; Virology; Viruses; Europe; enterovirus A71; enterovirus; parechovirus; PCR; RNA transcripts

Polymerase chain reaction (PCR) detection has become the gold standard for diagnosis and typing of enterovirus (EV) and human parechovirus (HPeV) infections. Its effectiveness depends critically on using the appropriate sample types and high assay sensitivity as viral loads in cerebrospinal fluid sa...