半胱氨酰白三烯受体1拮抗剂普鲁司特调节SK-N-SH细胞分化

Q256%Q421; 目的:观察半胱氨酰白三烯受体激动剂LTD4以及受体1(CysLT1)拮抗剂普鲁司特对人神经母细胞瘤细胞株SK-N-SH细胞分化的影响.方法:以维A酸为阳性对照,观察LTD4、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞形态学变化;用免疫印迹法观察SK-N-SH细胞CysLT1和CysLT2受体的表达;用免疫荧光法观察神经元分化标记物微管相关蛋白(MAP-2)的表达.结果:免疫印迹显示,SK-N-SH表达CysLT1受体和CysLT2受体,CysLT2受体表达较多.形态学结果显示,维A酸、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞发生形态学改变,表现为胞体变小...

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Published in浙江大学学报（医学版） Vol. 36; no. 2; pp. 123 - 129
Main Authors 彭芳, 方三华, 郑晓亮, 张纬萍, 卢韵碧, 魏尔清
Format Journal Article
LanguageChinese
Published 浙江大学医学院,药理学教研室,浙江,杭州,310058 2007
Subjects
神经母细胞瘤/病理学
普鲁司特
白三烯D4
白三烯类/治疗应用
细胞分化
受体,白三烯
SK-N-SH细胞
半胱氨酰白三烯受体
Online AccessGet full text
ISSN1008-9292
DOI10.3785/j.issn.1008-9292.2007.02.004

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Abstract Q256%Q421; 目的:观察半胱氨酰白三烯受体激动剂LTD4以及受体1(CysLT1)拮抗剂普鲁司特对人神经母细胞瘤细胞株SK-N-SH细胞分化的影响.方法:以维A酸为阳性对照,观察LTD4、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞形态学变化;用免疫印迹法观察SK-N-SH细胞CysLT1和CysLT2受体的表达;用免疫荧光法观察神经元分化标记物微管相关蛋白(MAP-2)的表达.结果:免疫印迹显示,SK-N-SH表达CysLT1受体和CysLT2受体,CysLT2受体表达较多.形态学结果显示,维A酸、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞发生形态学改变,表现为胞体变小,有明显的突起生长;而LTD4无明显作用.免疫荧光结果显示,在对照组和普鲁司特组MAP-2主要分布于胞体;在维甲酸组MAP-2除了分布于胞体外,还分布于突起.普鲁司特增加MAP-2阳性细胞数.结论:CysLT1受体拮抗剂普鲁司特参与SK-N-SH细胞分化的调节.
AbstractList Q256%Q421; 目的:观察半胱氨酰白三烯受体激动剂LTD4以及受体1(CysLT1)拮抗剂普鲁司特对人神经母细胞瘤细胞株SK-N-SH细胞分化的影响.方法:以维A酸为阳性对照,观察LTD4、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞形态学变化;用免疫印迹法观察SK-N-SH细胞CysLT1和CysLT2受体的表达;用免疫荧光法观察神经元分化标记物微管相关蛋白(MAP-2)的表达.结果:免疫印迹显示,SK-N-SH表达CysLT1受体和CysLT2受体,CysLT2受体表达较多.形态学结果显示,维A酸、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞发生形态学改变,表现为胞体变小,有明显的突起生长;而LTD4无明显作用.免疫荧光结果显示,在对照组和普鲁司特组MAP-2主要分布于胞体;在维甲酸组MAP-2除了分布于胞体外,还分布于突起.普鲁司特增加MAP-2阳性细胞数.结论:CysLT1受体拮抗剂普鲁司特参与SK-N-SH细胞分化的调节.
Author 郑晓亮
方三华
魏尔清
彭芳
张纬萍
卢韵碧
AuthorAffiliation 浙江大学医学院,药理学教研室,浙江,杭州,310058
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Keywords 神经母细胞瘤/病理学
普鲁司特
白三烯D4
白三烯类/治疗应用
细胞分化
受体,白三烯
SK-N-SH细胞
半胱氨酰白三烯受体
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Title 半胱氨酰白三烯受体1拮抗剂普鲁司特调节SK-N-SH细胞分化
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