半胱氨酰白三烯受体1拮抗剂普鲁司特调节SK-N-SH细胞分化

Q256%Q421; 目的:观察半胱氨酰白三烯受体激动剂LTD4以及受体1(CysLT1)拮抗剂普鲁司特对人神经母细胞瘤细胞株SK-N-SH细胞分化的影响.方法:以维A酸为阳性对照,观察LTD4、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞形态学变化;用免疫印迹法观察SK-N-SH细胞CysLT1和CysLT2受体的表达;用免疫荧光法观察神经元分化标记物微管相关蛋白(MAP-2)的表达.结果:免疫印迹显示,SK-N-SH表达CysLT1受体和CysLT2受体,CysLT2受体表达较多.形态学结果显示,维A酸、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞发生形态学改变,表现为胞体变小...

Full description

Saved in:
Bibliographic Details
Published in浙江大学学报（医学版） Vol. 36; no. 2; pp. 123 - 129
Main Authors 彭芳, 方三华, 郑晓亮, 张纬萍, 卢韵碧, 魏尔清
Format Journal Article
LanguageChinese
Published 浙江大学医学院,药理学教研室,浙江,杭州,310058 2007
Subjects
神经母细胞瘤/病理学
普鲁司特
白三烯D4
白三烯类/治疗应用
细胞分化
受体,白三烯
SK-N-SH细胞
半胱氨酰白三烯受体
Online AccessGet full text
ISSN1008-9292
DOI10.3785/j.issn.1008-9292.2007.02.004

Cover

Abstract Q256%Q421; 目的:观察半胱氨酰白三烯受体激动剂LTD4以及受体1(CysLT1)拮抗剂普鲁司特对人神经母细胞瘤细胞株SK-N-SH细胞分化的影响.方法:以维A酸为阳性对照,观察LTD4、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞形态学变化;用免疫印迹法观察SK-N-SH细胞CysLT1和CysLT2受体的表达;用免疫荧光法观察神经元分化标记物微管相关蛋白(MAP-2)的表达.结果:免疫印迹显示,SK-N-SH表达CysLT1受体和CysLT2受体,CysLT2受体表达较多.形态学结果显示,维A酸、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞发生形态学改变,表现为胞体变小,有明显的突起生长;而LTD4无明显作用.免疫荧光结果显示,在对照组和普鲁司特组MAP-2主要分布于胞体;在维甲酸组MAP-2除了分布于胞体外,还分布于突起.普鲁司特增加MAP-2阳性细胞数.结论:CysLT1受体拮抗剂普鲁司特参与SK-N-SH细胞分化的调节.
AbstractList Q256%Q421; 目的:观察半胱氨酰白三烯受体激动剂LTD4以及受体1(CysLT1)拮抗剂普鲁司特对人神经母细胞瘤细胞株SK-N-SH细胞分化的影响.方法:以维A酸为阳性对照,观察LTD4、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞形态学变化;用免疫印迹法观察SK-N-SH细胞CysLT1和CysLT2受体的表达;用免疫荧光法观察神经元分化标记物微管相关蛋白(MAP-2)的表达.结果:免疫印迹显示,SK-N-SH表达CysLT1受体和CysLT2受体,CysLT2受体表达较多.形态学结果显示,维A酸、普鲁司特、LTD4+普鲁司特诱导SK-N-SH细胞发生形态学改变,表现为胞体变小,有明显的突起生长;而LTD4无明显作用.免疫荧光结果显示,在对照组和普鲁司特组MAP-2主要分布于胞体;在维甲酸组MAP-2除了分布于胞体外,还分布于突起.普鲁司特增加MAP-2阳性细胞数.结论:CysLT1受体拮抗剂普鲁司特参与SK-N-SH细胞分化的调节.
Author 郑晓亮
方三华
魏尔清
彭芳
张纬萍
卢韵碧
AuthorAffiliation 浙江大学医学院,药理学教研室,浙江,杭州,310058
AuthorAffiliation_xml – name: 浙江大学医学院,药理学教研室,浙江,杭州,310058
Author_xml – sequence: 1
  fullname: 彭芳
– sequence: 2
  fullname: 方三华
– sequence: 3
  fullname: 郑晓亮
– sequence: 4
  fullname: 张纬萍
– sequence: 5
  fullname: 卢韵碧
– sequence: 6
  fullname: 魏尔清
BookMark eNo9T0tLw0AY3EMFa-2_8CaJ3-7m0RylqFWLHtp72E13pUVSMIjRmw2WJio9CJZePHuq0VNF-m-aR_-FEUUGZmAO89hAJbfvCoS2MKjUrOk7PbXrea6KAWqKRSyiEgBTBaICaCVU_vfXUdXzuhx0TM0CUEZHyWOUB-9p_Lq6i7PpYjkPs-AtGU-Wiyec3s_SaJKEg3Q6W33cJuN5Fn7mcZBHg9axcqK0GtnXMA9ektEweXjeRGuSnXui-qcV1N7fa9cbSvP04LC-21Qcg2qKEFxqEnROOtxxhDR1CcUeZmjUENSwOAC2uCDcAew4WBJpGZxQzgwTCGeCVtD2b-wVcyVzz-xe__LCLQrtm17H97l97fOf-1CQRr8BvCJrTA
ClassificationCodes Q256%Q421
ContentType Journal Article
Copyright Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
Copyright_xml – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
DBID 2B.
4A8
92I
93N
PSX
TCJ
DOI 10.3785/j.issn.1008-9292.2007.02.004
DatabaseName Wanfang Data Journals - Hong Kong
WANFANG Data Centre
Wanfang Data Journals
万方数据期刊 - 香港版
China Online Journals (COJ)
China Online Journals (COJ)
DatabaseTitleList
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
DocumentTitle_FL Cysteinyl leukotriene receptor 1 antagonist pranlukast modulates differentiation of SK-N-SH cells
EndPage 129
ExternalDocumentID zjdxxb_yxb200702004
GrantInformation_xml – fundername: 国家自然科学基金
  funderid: (30672449)
GroupedDBID 2B.
4A8
92I
93N
ALMA_UNASSIGNED_HOLDINGS
PSX
RPM
TCJ
ID FETCH-LOGICAL-c634-eebf4f05b2dbccef75f0051a6436e369b0019be2bc01cc1f2f96b23ba6702bae3
ISSN 1008-9292
IngestDate Thu May 29 04:07:20 EDT 2025
IsPeerReviewed false
IsScholarly true
Issue 2
Keywords 神经母细胞瘤/病理学
普鲁司特
白三烯D4
白三烯类/治疗应用
细胞分化
受体,白三烯
SK-N-SH细胞
半胱氨酰白三烯受体
Language Chinese
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c634-eebf4f05b2dbccef75f0051a6436e369b0019be2bc01cc1f2f96b23ba6702bae3
PageCount 7
ParticipantIDs wanfang_journals_zjdxxb_yxb200702004
PublicationCentury 2000
PublicationDate 2007
PublicationDateYYYYMMDD 2007-01-01
PublicationDate_xml – year: 2007
  text: 2007
PublicationDecade 2000
PublicationTitle 浙江大学学报（医学版）
PublicationTitle_FL JOURNAL OF ZHEJIANG UNIVERSITY(MEDICAL SCIENCES)
PublicationYear 2007
Publisher 浙江大学医学院,药理学教研室,浙江,杭州,310058
Publisher_xml – name: 浙江大学医学院,药理学教研室,浙江,杭州,310058
SSID ssib051373730
ssib002263662
ssib009993744
ssib056826211
ssib058720545
ssib006576290
ssib006704889
ssj0002507821
ssib006562844
ssib002039758
ssib008143987
ssib008679799
ssib001051301
ssib001104240
Score 1.7086949
Snippet Q256%Q421;...
SourceID wanfang
SourceType Aggregation Database
StartPage 123
Title 半胱氨酰白三烯受体1拮抗剂普鲁司特调节SK-N-SH细胞分化
URI https://d.wanfangdata.com.cn/periodical/zjdxxb-yxb200702004
Volume 36
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnR1NT9VAsEFMiBfjZ_wOB_ZYbLftfhzb90oIBi9iwo10263GwyNRSJCDibxIBDUcTCRcPHtC9IQx_BveB7_AqzPb0teHxKB5SbNvd3Z2PtrOzH5MLWvCy5KEqTSxpWCZjQtnNqiZ27nOpaMzPDeAB4VnH7Lpx_7MfDA_cu5XbdfS8pKaTFdPPVfyP1qFOtArnpL9B81WSKECyqBfuIKG4XomHZM4IKJJREhiQYRHIpfEjEQOCQWJJREBlmNOpCRRk8Q-iQBMYg0Ah1Om-xSR3DQ1ifRMfxGRMDaF0LQF2EdQrAFE2AToKLigZX9AihihUiIdMCRgR4JC6PXogQ3v7GmEiCIiWEmpjE1nYWqg0CDFF2eP_WTDR4DjYcEl0hAb-iQ0BIVNErJawdAaBiQGahqItsAJIw5gDInYVMBUEyAIAswDVEFz5A1agGNm2KpEVwg8HoBIw45bSkd6BhaIiYfwN0hoNIEtDRxIgpSaQ1MuvHo8zsr8SQ6l0Q-Fp8aw0jQq5maoYWHJAjlHoqRvmmLEjx3_GLqolIWEgBWOyqMNXLIJ6jYNd7iAFzxk9IqsM-XDTWsWzC2Of5fOkFtMR520sx4XgbGzOMBkNUCZERRT4PoD_6La9bn6LFtZUQsvVxTCOdTk8T1POXdxL-7sq7g2TxGAb1XPS4gr9DU_1QE3uracDzGEx2p5LyFIAbfLr_0Hm19bDmYcDdfAjxcQNchanIJZKOvL3RK9-AE-II7Dr8IXMAjS6cBQBoJTCIWCarIX4gzwxM3kz7GsxqyJUpD3_yZGc5awlSetJzW3d-6SdbGMV8fD4uVz2RpZfXrFGpstd-RctWY6Hzb77W_dvS9Hb_Z6OweH-xu99tfO1vbhwUe3-263u7nd2Vjr7uwefX_d2drvbfzo77X7m2vle6H3c73f_tx5u955_-maNTcVzzWm7fL7LHbKPN_WWuV-7gSKZipNdc6DHE18AjEO0x7DZKuuVJqq1HHT1M1pLpminkpA-lQl2rtujbYWW_qGNa59nSmmuUoS7jMlhXIyXzsqSanIXKFvWhOlFBbK1--LhVPupltnA7ttXShWaHAi9Y41uvR8Wd-F0GJJ3TO34W8gl9hQ
linkProvider ISSN International Centre
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%8D%8A%E8%83%B1%E6%B0%A8%E9%85%B0%E7%99%BD%E4%B8%89%E7%83%AF%E5%8F%97%E4%BD%931%E6%8B%AE%E6%8A%97%E5%89%82%E6%99%AE%E9%B2%81%E5%8F%B8%E7%89%B9%E8%B0%83%E8%8A%82SK-N-SH%E7%BB%86%E8%83%9E%E5%88%86%E5%8C%96&rft.jtitle=%E6%B5%99%E6%B1%9F%E5%A4%A7%E5%AD%A6%E5%AD%A6%E6%8A%A5%EF%BC%88%E5%8C%BB%E5%AD%A6%E7%89%88%EF%BC%89&rft.au=%E5%BD%AD%E8%8A%B3&rft.au=%E6%96%B9%E4%B8%89%E5%8D%8E&rft.au=%E9%83%91%E6%99%93%E4%BA%AE&rft.au=%E5%BC%A0%E7%BA%AC%E8%90%8D&rft.date=2007&rft.pub=%E6%B5%99%E6%B1%9F%E5%A4%A7%E5%AD%A6%E5%8C%BB%E5%AD%A6%E9%99%A2%2C%E8%8D%AF%E7%90%86%E5%AD%A6%E6%95%99%E7%A0%94%E5%AE%A4%2C%E6%B5%99%E6%B1%9F%2C%E6%9D%AD%E5%B7%9E%2C310058&rft.issn=1008-9292&rft.volume=36&rft.issue=2&rft.spage=123&rft.epage=129&rft_id=info:doi/10.3785%2Fj.issn.1008-9292.2007.02.004&rft.externalDocID=zjdxxb_yxb200702004
thumbnail_s http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fzjdxxb-yxb%2Fzjdxxb-yxb.jpg