Single-molecule protein identification by sub-nanopore sensors

• Published in: PLoS computational biology Vol. 13; no. 5; p. e1005356
• Main Authors: Kolmogorov, Mikhail, Kennedy, Eamonn, Dong, Zhuxin, Timp, Gregory, Pevzner, Pavel A.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.05.2017; Public Library of Science (PLoS)
• Subjects: Algorithms; Artificial intelligence; Bacteria; Biology and Life Sciences; Colleges & universities; Computer engineering; Computer science; Databases, Protein; Deoxyribonucleic acid; DNA; DNA sequencing; Electric currents; Electrical engineering; Fragmentation; Gene sequencing; Identification; Ions; Mass spectrometry; Mass spectroscopy; Matching; Methods; Nanopores; Nanostructure; Nanotechnology - methods; Observations; Physical Sciences; Porosity; Protein-protein interactions; Proteins; Proteins - chemistry; Proteins - classification; Proteomics; Research and Analysis Methods; Scientific imaging; Sensitivity; Sensors; Silicon nitride; Software; Technology; Translocation; La Jolla California; California; Indiana; United States > US

Recent advances in top-down mass spectrometry enabled identification of intact proteins, but this technology still faces challenges. For example, top-down mass spectrometry suffers from a lack of sensitivity since the ion counts for a single fragmentation event are often low. In contrast, nanopore t...