Evaluation of Fluorescence Polarization Method for Binding Study in Carbohydrate-Lectin Interaction

• Published in: Biological & pharmaceutical bulletin Vol. 21; no. 11; pp. 1215 - 1217
• Main Authors: ODA, Yasuo, KINOSHITA, Mitsuhiro, NAKAYAMA, Katsuyoshi, KAKEHI, Kazuaki
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.11.1998; Maruzen; Japan Science and Technology Agency
• Subjects: Analysis; Binding Sites; Biological and medical sciences; Carbohydrate Metabolism; Carbohydrate Sequence; Evaluation Studies as Topic; fluorescence polarization; Fluorescence Polarization - methods; General pharmacology; Glycine max; Glycopeptides - metabolism; lectin; Lectins - metabolism; mannan; Medical sciences; Molecular Sequence Data; Pharmacology. Drug treatments; Plant Lectins; Polysaccharides - metabolism; Saccharomyces cerevisiae; soybean agglutinin; Triticum; yeast; Lectin; Fluorescence detector; Carbon compound; Yeast; Polarization analyzer; Molecular interaction; In vitro; Medicinal plant; Glycopeptide; Plant origin; Polysaccharide; Quantitative analysis; Hydrates
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.21.1215

The fluorescence polarization (FP) technique was evaluated to determine molecular interaction between plant lectins and polysaccharides, yeast cells and glycopeptide after labeling the lectins with fluorescein isothiocyanate. Use of Lycoris radiata agglutinin allowed determination of the molecular interactions with large biomolecules containing mannose oligomers and polymers. Another example using a fluorescein-labeled glycopeptide also indicated that use of the FP method would allow easy observation of the molecular interactions on the quantitative base. The present technique is highly sensitive and facile because it does not require any washing procedures before measurement.