Improvement in endothelial cell adhesion and retention under physiological shear stress using a laminin–apatite composite layer on titanium

• Published in: Journal of the Royal Society interface Vol. 10; no. 81; p. 20130014
• Main Authors: He, Fupo, Wang, Xiupeng, Maruyama, Osamu, Kosaka, Ryo, Sogo, Yu, Ito, Atsuo, Ye, Jiandong
• Format: Journal Article
• Language: English
• Published: England The Royal Society 06.04.2013
• Subjects: Albumins - chemistry; Apatite; Apatites - chemistry; Biocompatible Materials; Biotechnology - methods; Cell Adhesion; Cell Adhesion - physiology; Cell Retention; Endothelialization; Human Umbilical Vein Endothelial Cells - physiology; Humans; Laminin; Laminin - chemistry; Shear Strength - physiology; Titanium; Titanium - chemistry

Apatite (Ap), laminin–apatite composite (L5Ap, L10Ap, L20Ap and L40Ap) and albumin–apatite (AlbAp) composite layers were prepared on titanium (Ti) using a supersaturated calcium phosphate solution supplemented with laminin (0, 5, 10, 20 and 40 μg ml−1) or albumin (800 μg ml−1). With an increase in t...